breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 123,061 C→T R15R (CGC→CGT aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
JC JC 168,640 IS2 (+) +5 bp coding (1157‑1161/2244 nt) fhuA → ferrichrome outer membrane transporter
MC JC 257,908 Δ776 bp [crl] [crl]
RA 453,291 G→A L85L (CTC→CTT ampG ← muropeptide transporter
RA 909,353 G→A A566V (GCG→GTG)  poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
JC JC 911,086 IS2 (–) +5 bp intergenic (‑37/+92) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
RA 1,158,881 C→T S338L (TCG→TTG)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
JC JC 1,290,476 IS3 (–) +3 bp coding (235‑237/1014 nt) rssB → PcnB‑degradosome interaction factor; response regulator
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,413,461 G→A intergenic (‑329/‑9) yfbV ← / → ackA UPF0208 family inner membrane protein/acetate kinase A and propionate kinase 2
JC 2,722,123 Δ7 bp coding (2171‑2177/2661 nt) pka → protein lysine acetyltransferase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC JC 3,771,708 IS3 (–) +4 bp :: +TC coding (34‑37/363 nt) yibI ← DUF3302 family inner membrane protein
RA 3,798,659 (T)8→7 coding (654/1074 nt) waaU ← lipopolysaccharide core biosynthesis
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
MC JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 123064 127536 4473 6 [4] [5] 7 [aceE]–[aceF] [aceE],[aceF]
* * ÷ NC_000913 1209603 1209618 16 29 [0] [0] 27 stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* * ÷ NC_000913 2066289–2066464 2066606–2066468 5–318 7 [5] [5] 8 [insH1] [insH1]
* * ÷ NC_000913 3423753–3424234 3424519–3424238 5–767 8 [5] [5] 8 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1097245 =NA (NA)5 (0.180) 5/248 3.0 29.8% intergenic (+416/+320) ycdU/serX putative inner membrane protein/tRNA‑Ser
?NC_000913 1097274 = 12 (0.420)intergenic (+445/+291) ycdU/serX putative inner membrane protein/tRNA‑Ser
* ? NC_000913 1207790 =1 (0.030)20 (0.790) 19/220 0.4 97.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 0 (0.000)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078051 (0.030)27 (1.070) 25/220 0.2 98.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 0 (0.000)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 404068319 (0.660)16 (0.560) 9/248 1.9 44.6% intergenic (+47/+223) rrfA/mobB 5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B
?NC_000913 = 4213162 21 (0.740)intergenic (+3/‑72) rrfE/yjaA 5S ribosomal RNA of rrnE operon/stress‑induced protein