Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,413,461	G→A	intergenic (‑329/‑9)	yfbV ← / → ackA	UPF0208 family inner membrane protein/acetate kinase A and propionate kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,413,461	0	G	A	100.0%	83.9 / NA	25	intergenic (‑329/‑9)	yfbV/ackA	UPF0208 family inner membrane protein/acetate kinase A and propionate kinase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/11);  total (14/11)

ATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCT  >  NC_000913/2413344‑2413585                                                                                                                      |                                                                                                                             atTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGt                                                                                               >  1:80544/1‑149 (MQ=255) atTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGt                                                                                               >  1:82869/1‑149 (MQ=255)    tcttcCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCt                                                                                            >  6:85730/1‑149 (MQ=255)                       aaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCa                                                                         <  2:80544/149‑1 (MQ=255)                       aaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCa                                                                         <  2:82869/149‑1 (MQ=255)                                  cATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGc                                                              <  5:85730/149‑1 (MQ=255)                                   atgatgTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCa                                                                         <  8:35442/137‑1 (MQ=255)                                   atgatgTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCa                                                                         >  7:35442/1‑137 (MQ=255)                                               aTAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATg                                                   >  7:47920/1‑147 (MQ=255)                                                        ggTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCg                                                                                    <  4:89378/105‑1 (MQ=255)                                                        ggTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCg                                                                                    >  3:89378/1‑105 (MQ=255)                                                            tcatcaTGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTg                                       >  3:54186/1‑146 (MQ=255)                                                              atcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCca                                                            <  4:5076/123‑1 (MQ=255)                                                              atcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCca                                                            >  3:5076/1‑123 (MQ=255)                                                               tcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCg                                                      >  3:36836/1‑128 (MQ=255)                                                               tcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCg                                                      <  4:36836/128‑1 (MQ=255)                                                               tcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATg                                                   >  1:119751/1‑131 (MQ=255)                                                               tcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATg                                                   <  2:119751/131‑1 (MQ=255)                                                                       tACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTg                                                               <  2:144279/111‑1 (MQ=255)                                                                       tACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTg                                                               >  1:144279/1‑111 (MQ=255)                                                                          gCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTgg                                                                                                >  2:74971/1‑75 (MQ=255)                                                                          gCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTgg                                                                                                <  1:74971/75‑1 (MQ=255)                                                                                       cTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGa                                                     <  2:135099/105‑1 (MQ=255)                                                                                       cTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGa                                                     >  1:135099/1‑105 (MQ=255)                                                                                              ttttttAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCt  >  5:119122/1‑149 (MQ=255)                                                                                                                      |                                                                                                                             ATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCT  >  NC_000913/2413344‑2413585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑