Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,290,476 IS3 (–) +3 bp coding (235‑237/1014 nt) rssB → PcnB‑degradosome interaction factor; response regulator

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1094245 =NA (NA)13 (0.450) 12/252 1.4 100% noncoding (1258/1258 nt) IS3 repeat region
?NC_000913 = 1290478 0 (0.000)coding (237/1014 nt) rssB PcnB‑degradosome interaction factor; response regulator
* ? NC_000913 = 1095502NA (NA)44 (1.520) 34/252 0.1 100% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 1290476 = 0 (0.000)coding (235/1014 nt) rssB PcnB‑degradosome interaction factor; response regulator

TGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCT  >  NC_000913/1290330‑1290612
                                                                                                                                                  |                                                                                                                                        
agcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaca                                                                                                                                         <  2:33352‑M2/2‑1 (MQ=255)
            ggcttcacttcgaaattcaggctaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgaggcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTg                                                                                                                            <  8:75175‑M2/15‑1 (MQ=255)
                    tgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATc                                                                                                                    >  1:149766‑M2/127‑149 (MQ=255)
                      cgaaattcaggcgaatgctgtttacgggggtttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCt                                                                                                                   <  3:81358‑M2/24‑1 (MQ=255)
                       gaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGc                                                                                                                 >  6:132207‑M2/124‑149 (MQ=255)
                            tGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGtgatcctacccacgtaatatggacacag                                                                                                            >  2:85586‑M2/1‑121 (MQ=255)
                                gcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGaaaa                                                                                                        >  6:55886‑M2/115‑149 (MQ=255)
                                    atgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATg                                                                                                    <  5:55886‑M2/39‑1 (MQ=255)
                                             acggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCGCTGAAAATATGGCAGATAtt                                                                                           >  7:30445‑M2/102‑149 (MQ=255)
                                               ttGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGtgatcc                                                                                                                                  >  6:103189‑M2/1‑102 (MQ=255)
                                               ttGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGtgatcc                                                                                                                                  <  5:103189‑M2/108‑7 (MQ=255)
                                                   tGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAg                                                                                                                                        <  8:47837/98‑1 (MQ=255)
                                                   tGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAg                                                                                                                                        >  7:47837/1‑98 (MQ=255)
                                                   tGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGtgatcctacccacgtaatat                                                                                                                    >  8:94749‑M2/1‑98 (MQ=255)
                                                   tGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGtgatcctacccacgtaatat                                                                                                                    <  7:94749‑M2/118‑21 (MQ=255)
                                                         actggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCGCTGAAAATATGGCAGATATTGCCAAAGCGtt                                                                                <  8:30445‑M2/59‑1 (MQ=255)
                                                             gttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTg                                                                                          >  4:93822‑M2/86‑134 (MQ=255)
                                                             gttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTg                                                                                          <  3:93822‑M2/49‑1 (MQ=255)
                                                             gttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTc                                                                           >  2:125497‑M2/86‑149 (MQ=255)
                                                             cACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGtgatcctacccacgtaatatggacacaggcctaa                                                                                                      <  2:58817‑M2/122‑35 (MQ=255)
                                                             cACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGtgatcctacccacgtaatatggacacaggcctaa                                                                                                      >  1:58817‑M2/1‑88 (MQ=255)
                                                                     tgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtatgatcac                                                                                                                                          >  1:19350‑M2/78‑78 (MQ=255)
                                                                     tgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtatgatcac                                                                                                                                          <  2:19350‑M2/1‑1 (MQ=255)
                                                                     tgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTgg                                                                        >  6:41354‑M2/78‑144 (MQ=255)
                                                                     tgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTgg                                                                        <  5:41354‑M2/67‑1 (MQ=255)
                                                                       tttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATgg                                                                                                   >  8:33754‑M2/76‑115 (MQ=255)
                                                                             tcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGt                                                           >  4:38607‑M2/70‑149 (MQ=255)
                                                                                tgtgATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGtgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgag                                                                   >  2:43644‑M2/1‑69 (MQ=255)
                                                                                tgtgATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGtgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgag                                                                   <  1:43644‑M2/138‑70 (MQ=255)
                                                                                  tgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTg                                                       <  5:132207‑M2/84‑1 (MQ=255)
                                                                                     gtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTg                                                    >  2:101998‑M2/62‑148 (MQ=255)
                                                                                     gtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTg                                                    >  2:99799‑M2/62‑148 (MQ=255)
                                                                                             tgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGt                                                           >  2:148739‑M2/54‑133 (MQ=255)
                                                                                             tgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGt                                                           <  1:148739‑M2/80‑1 (MQ=255)
                                                                                                 tgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTa                                          >  8:6430‑M2/50‑146 (MQ=255)
                                                                                                    aaTGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGtgatcctacccacgtaatatggacacaggcctaagcgaggttctt                                                                                           >  5:21307‑M2/1‑49 (MQ=255)
                                                                                                    aaTGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGtgatcctacccacgtaatatggacacaggcctaagcgaggttctt                                                                                           <  6:21307‑M2/94‑46 (MQ=255)
                                                                                                      gtttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCt                                   >  7:9610‑M2/45‑148 (MQ=255)
                                                                                                       tttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGa                                 <  1:101998‑M2/106‑1 (MQ=255)
                                                                                                       tttactcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGa                                 <  1:99799‑M2/106‑1 (MQ=255)
                                                                                                          actcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATgg                                                                                                   >  2:152127‑M2/41‑80 (MQ=255)
                                                                                                          actcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATgg                                                                                                   <  1:154322‑M2/40‑1 (MQ=255)
                                                                                                          actcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATgg                                                                                                   <  1:152127‑M2/40‑1 (MQ=255)
                                                                                                          actcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATgg                                                                                                   >  2:154322‑M2/41‑80 (MQ=255)
                                                                                                            tcacttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGc                            >  1:59808‑M2/39‑149 (MQ=255)
                                                                                                              acttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGc                            <  1:125497‑M2/111‑1 (MQ=255)
                                                                                                                ttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGa                                 >  5:79590‑M2/35‑140 (MQ=255)
                                                                                                                ttagccgcgtgtccactattgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGa                                 <  6:79590‑M2/106‑1 (MQ=255)
                                                                                                                                  ttgctgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGc        <  2:59808‑M2/131‑1 (MQ=255)
                                                                                                                                      tgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTAc                                                                              <  1:50831‑M2/61‑1 (MQ=255)
                                                                                                                                      tgggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTAc                                                                              >  2:50831‑M2/13‑73 (MQ=255)
                                                                                                                                        ggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGtct  <  2:149766‑M2/137‑1 (MQ=255)
                                                                                                                                        ggtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGtct  <  3:38607‑M2/137‑1 (MQ=255)
                                                                                                                                         gtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGc                            >  6:26028‑M2/10‑120 (MQ=255)
                                                                                                                                         gtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGc                            <  5:26028‑M2/111‑1 (MQ=255)
                                                                                                                                         gtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGttt            >  7:5958‑M2/10‑136 (MQ=255)
                                                                                                                                         gtaagatcaCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGttt            <  8:5958‑M2/127‑1 (MQ=255)
                                                                                                                                         agagGCGACCAGtgatcctacccacgtaatatggacacaggcctaagcgaggttctt                                                                                           >  2:10238‑M2/1‑12 (MQ=255)
                                                                                                                                         agagGCGACCAGtgatcctacccacgtaatatggacacaggcctaagcgaggttctt                                                                                           <  1:10238‑M2/57‑46 (MQ=255)
                                                                                                                                                  |                                                                                                                                        
TGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAAAGCGTTACGTCTGGGCGTTGAAGATGTTTTGCTGAAACCAGTTAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCT  >  NC_000913/1290330‑1290612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.