Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,798,659	(T)8→7	coding (654/1074 nt)	waaU ←	lipopolysaccharide core biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,798,652	0	T	.	100.0%	123.1 / NA	28	coding (661/1074 nt)	waaU	lipopolysaccharide core biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/14);  total (14/14)

ATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCA  >  NC_000913/3798509‑3798786                                                                                                                                                |                                                                                                                                       atcaAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttt                                                                                                                                   >  1:7951/1‑149 (MQ=255)              gtTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttt                                                                                                                                   >  1:124361/1‑136 (MQ=255)              gtTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttt                                                                                                                                   <  2:124361/136‑1 (MQ=255)               tttCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttttgc                                                                                                                                <  1:64458/138‑3 (MQ=255)               tttCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttttgc                                                                                                                                >  2:64458/1‑136 (MQ=255)                              ttggtattgTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTc                                                                                                     <  6:83560/149‑1 (MQ=255)                                  tattgTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGt                                                                                                 <  6:17704/149‑1 (MQ=255)                                         aaTAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTcc                                                                                           <  2:7951/148‑1 (MQ=255)                                         aaTAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTCCACCTAATGGATAAATAATGCATATACTTGTagcacc                                                                                           >  6:64963/1‑143 (MQ=255)                                          aTAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCaa                                                                                         >  7:42840/1‑149 (MQ=255)                                                 ttGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTc                                                                                  >  6:87385/1‑149 (MQ=255)                                                      gTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCtttta                                                                             >  6:44818/1‑149 (MQ=255)                                                                 aaTATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTc                                                                   <  5:87385/148‑1 (MQ=255)                                                                        tCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttatttt                                                                         >  5:117881/1‑135 (MQ=255)                                                                        tCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttatttt                                                                         <  6:117881/135‑1 (MQ=255)                                                                        tCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAAc                                                                >  3:75867/1‑144 (MQ=255)                                                                        tCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAAc                                                                <  4:75867/144‑1 (MQ=255)                                                                                    cAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGc                                                <  8:42840/148‑1 (MQ=255)                                                                                            gtTTTCACTTCTTGATATATAACTTTTATTTGCTCAACCGTAAGACGGCATA‑TTTTTTTGCACATAATGGATTAATAATGACTATTCTTGTATCTCCAATAACTTCTTATATTTTATCTTCAACATCTAATGGTAAATGCAAGTCGTAg                                       >  4:79164/1‑149 (MQ=255)                                                                                             ttttCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttatttt                                                                         >  2:124741/1‑114 (MQ=255)                                                                                             ttttCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttatttt                                                                         <  1:124741/114‑1 (MQ=255)                                                                                             ttttCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCa                                                                  <  2:54721/121‑1 (MQ=255)                                                                                             ttttCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCa                                                                  >  1:54721/1‑121 (MQ=255)                                                                                                 cACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACAt                                                              <  5:28084/121‑1 (MQ=255)                                                                                                 cACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACAt                                                              >  6:28084/1‑121 (MQ=255)                                                                                                      cttGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGt                                      >  7:42364/1‑140 (MQ=255)                                                                                                      cttGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGt                                      <  8:42364/140‑1 (MQ=255)                                                                                                                      tATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCAt              <  5:64963/148‑1 (MQ=255)                                                                                                                               aaaCGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCtt    >  8:48800/1‑149 (MQ=255)                                                                                                                               aaaCGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATGTa                        >  8:100075/1‑129 (MQ=255)                                                                                                                                 aCGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCa  <  5:44818/149‑1 (MQ=255)                                                                                                                                        ccGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCtt    >  8:77251/2‑140 (MQ=255)                                                                                                                                        aCGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCtt    <  7:77251/140‑1 (MQ=255)                                                                                                                                                |                                                                                                                                       ATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCA  >  NC_000913/3798509‑3798786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑