Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,158,881	C→T	S338L (TCG→TTG)	ptsG →	fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,158,881	0	C	T	100.0%	70.6 / NA	21	S338L (TCG→TTG)	ptsG	fused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/12);  total (9/12)

ACCTCGTTCCTGACCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCG  >  NC_000913/1158736‑1159020                                                                                                                                                  |                                                                                                                                            aCCTCGTTCCTGACCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCa                                                                                                                                          <  1:64473/149‑1 (MQ=255)  ccTCGTTCCTGACCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCAc                                                                                                                                         <  1:77007/149‑1 (MQ=255)           tGACCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATc                                                                                                                                >  4:64165/1‑149 (MQ=255)            gACCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGc                                                                                                                                           <  8:68615/137‑1 (MQ=255)            gACCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGc                                                                                                                                           >  7:68615/1‑137 (MQ=255)                         gAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTtct                                                                                                                  <  8:36000/149‑1 (MQ=255)                               aTCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTgg                                                                                                            >  1:121920/1‑149 (MQ=255)                               aTCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTgg                                                                                                            <  3:64165/149‑1 (MQ=255)                                          cTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTACTTGCACGGTCTCATCGACTTCATCGTTCTGTCTGGTAACagcagc                                                                                                  >  5:90881/1‑148 (MQ=255)                                           ttCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGt                                                                                                           <  2:33090/138‑1 (MQ=255)                                           ttCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGt                                                                                                           >  1:33090/1‑138 (MQ=255)                                                gTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACtgt                                                                                           >  7:54594/1‑149 (MQ=255)                                                   cGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTgg                                                                                         <  8:54594/148‑1 (MQ=255)                                                                                                      cccAATCTGTATTCTTCTGGGGATGCGGGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCGGAGCGTCGGTATCGGTTATGCGATTGTTTTCTACACCATCTTc                                      <  6:57240/148‑1 (MQ=255)                                                                                                         aaTCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTtac                                                  >  7:20861/1‑133 (MQ=255)                                                                                                         aaTCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTtac                                                  <  8:20861/133‑1 (MQ=255)                                                                                                                aTTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGAtt                                                        <  7:33770/120‑1 (MQ=255)                                                                                                                aTTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGAtt                                                        >  8:33770/1‑120 (MQ=255)                                                                                                                                gTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCt            >  4:106285/1‑148 (MQ=255)                                                                                                                                 tGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGa          <  2:121920/149‑1 (MQ=255)                                                                                                                                         cgtcgtTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCg  <  3:106285/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                            ACCTCGTTCCTGACCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCG  >  NC_000913/1158736‑1159020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑