Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,798,659 (T)8→7 coding (654/1074 nt) waaU ← lipopolysaccharide core biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,798,6520T.98.2% 743.5 / 6.7 167coding (661/1074 nt)waaUlipopolysaccharide core biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base . (85/79);  total (87/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 176 total aligned reads displayed.

CTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTAT  >  NC_000913/3798506‑3798796
                                                                                                                                                  |                                                                                                                                                
cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAttt                                                                                                                                                >  7:402890/1‑149 (MQ=255)
cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAttt                                                                                                                                                >  5:134879/1‑149 (MQ=255)
   atcaAATGGTAGTGTTTCTATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACACTGTAAATATAATCCGATAATATTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttt                                                                                                                                             <  1:260479/149‑1 (MQ=255)
   atcaAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttt                                                                                                                                             >  4:231828/1‑149 (MQ=255)
   atcaAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttt                                                                                                                                             <  2:127586/149‑1 (MQ=255)
    tcaAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAttttttt                                                                                                                                            >  7:143959/1‑149 (MQ=255)
    tcaAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAttttttt                                                                                                                                            <  1:277576/149‑1 (MQ=255)
            tAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTaa                                                                                                                                   <  3:122642/149‑1 (MQ=255)
              gtgtTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTATTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATg                                                                                                                                 >  1:412380/1‑149 (MQ=255)
               tgtTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCTGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGAACCTAATg                                                                                                                                 >  8:119334/1‑148 (MQ=255)
                gtTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTATTGGa                                                                                                                               >  7:241637/1‑149 (MQ=255)
                gtTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATgg                                                                                                                                >  3:377629/1‑148 (MQ=255)
                gtTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATgg                                                                                                                                >  3:371634/1‑148 (MQ=255)
                gtTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATgg                                                                                                                                <  4:371634/148‑1 (MQ=255)
                gtTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGa                                                                                                                               >  6:141996/1‑149 (MQ=255)
                 tttCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGa                                                                                                                               <  3:78533/148‑1 (MQ=255)
                 tttCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGAt                                                                                                                              <  4:71119/149‑1 (MQ=255)
                 tttCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGAt                                                                                                                              >  3:174466/1‑149 (MQ=255)
                 tttCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACACGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATgg                                                                                                                                <  8:139246/147‑1 (MQ=255)
                      aTTTCAAGTATTGGTATTGTCAATAAATCTGGCGGTAACCCGGTAAATATAATCCGATATTTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCGCATACGTAAGACGGCATATTTTTTTGGAGCTAATGGATtatt                                                                                                                          >  2:179789/1‑146 (MQ=255)
                       tttCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATtaataa                                                                                                                        >  3:112265/1‑149 (MQ=255)
                           aaGTATTGGTATTGTCAATAAATCTTGCGGAAACCCGGTAAATATAATCAGAAAATTTTCAAAGTGTGTTTTCACTTCTTGATAAATAACTTTTATTTTCTCAAACGAAAGACGGCATATTTTTTTGCACCTaa                                                                                                                                   <  5:177842/134‑1 (MQ=255)
                                  ggtattgTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATg                                                                                                                      >  6:299998/1‑140 (MQ=255)
                                  ggtattgTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATg                                                                                                                      <  5:299998/140‑1 (MQ=255)
                                  ggtattgTCAATAAATCTTGCGGTAACCCGGTAAATATAATCAGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCt                                                                                                              <  4:109666/148‑1 (MQ=255)
                                   gtattgTCAATAAATGTTGCGGTAACACGGTAAATATAATCCGATAATTTTGAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCtt                                                                                                             <  5:50141/148‑1 (MQ=255)
                                   gtattgTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATg                                                                                                                                 >  8:195320/1‑128 (MQ=255)
                                   gtattgTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATg                                                                                                                                 <  7:195320/128‑1 (MQ=255)
                                    tattgTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTg                                                                                                            >  7:360897/1‑148 (MQ=255)
                                    tattgTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTg                                                                                                            >  7:251911/1‑148 (MQ=255)
                                    tattgTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTg                                                                                                            >  8:439796/1‑148 (MQ=255)
                                     attgTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAACGTGTGGTTTCCCTGCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTATTATGACCCTAAGGGATTAATAATGACTAGACTTGTa                                                                                                          >  2:352567/1‑149 (MQ=255)
                                        gTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACATAATGGATTAATAATGACTATTCTTGTAtct                                                                                                       >  1:323952/1‑149 (MQ=255)
                                        gTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATATTCAAAGTGTCTTTTCACTTCTTGATATATAACTTTAAGTTGCTCAAACGTAATACGGCATATTTTTTTTCACCTAATCGATTAATAATGACTATTCTTGAAtct                                                                                                       >  4:152590/1‑149 (MQ=255)
                                           aaTAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCtt                                                                                                             <  7:320572/140‑1 (MQ=255)
                                           aaTAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCtt                                                                                                             >  8:320572/1‑140 (MQ=255)
                                            aTAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCaa                                                                                                   >  4:371007/1‑149 (MQ=255)
                                            aTAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCaa                                                                                                   >  1:139357/1‑149 (MQ=255)
                                               aaTCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATa                                                                                                 >  2:175306/1‑148 (MQ=255)
                                                aTCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACTGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATaaa                                                                                               >  1:396171/1‑149 (MQ=255)
                                                aTCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATaaa                                                                                               >  5:249995/1‑149 (MQ=255)
                                                  cTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGAc                                                                                                                    >  4:167486/1‑126 (MQ=255)
                                                     gCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAAtt                                                                                             <  5:378238/146‑1 (MQ=255)
                                                      cGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttt                                                                                         >  2:294268/1‑149 (MQ=255)
                                                       ggTAACCCGGTAAATATAATCAGATAATTTTCAAAGTGTGTGTTCACTTCTTGATATATAACTTTTATTTTCTCAAACGTACGACTGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCtttt                                                                                        <  5:90185/149‑1 (MQ=255)
                                                        gTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCtttta                                                                                       <  6:331285/149‑1 (MQ=255)
                                                         tAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATAGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttat                                                                                      <  7:55721/149‑1 (MQ=255)
                                                            cccTGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttatttt                                                                                   <  5:283564/149‑1 (MQ=255)
                                                            cccGGTAAATATAATCCGATAATTTCCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttatttt                                                                                   <  4:131189/149‑1 (MQ=255)
                                                              cGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGt                                                                                                           >  7:446326/1‑123 (MQ=255)
                                                              cGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTtttattttat                                                                                 <  8:256373/149‑1 (MQ=255)
                                                               ggTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTtttattttat                                                                                 >  7:65129/1‑149 (MQ=255)
                                                               ggTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCaa                                                                                                   <  6:191928/130‑1 (MQ=255)
                                                               ggTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATc                                                                                <  6:134879/149‑1 (MQ=255)
                                                               ggTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATc                                                                                <  7:25900/149‑1 (MQ=255)
                                                                    atatAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCaa                                                                           <  1:191757/149‑1 (MQ=255)
                                                                       taATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACAt                                                                        <  5:156043/149‑1 (MQ=255)
                                                                          tCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTa                                                                     <  7:39578/149‑1 (MQ=255)
                                                                            cGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACt                                                                   >  3:364153/1‑149 (MQ=255)
                                                                            cGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACt                                                                   <  8:402890/149‑1 (MQ=255)
                                                                             gctAATTTTCAAAGTGGGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAACCGTAAGACGGCATATTTTTTTGCACCTCATGGATTAATAATGACTCTTCTTGTATCGCCAATAAATTCTTTTATTTTATCTTCAACATCTACTg                                                                  <  1:352567/147‑1 (MQ=255)
                                                                              aTAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTgg                                                                 >  5:165159/1‑149 (MQ=255)
                                                                              aTAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTgg                                                                 >  2:214864/1‑149 (MQ=255)
                                                                                  ttttCAAAGTGTGTTTTCACTTCTTGATATATATCTTTTATTTGCTCAAACGTAAGACTGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTGTTTTATTTTATCTTCAACATCTACTGGTaaa                                                             <  3:152590/149‑1 (MQ=255)
                                                                                  ttttCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAGGACGGCATATTTTTTTGCACCTAATGGATTAATAATGAGTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTaaa                                                             >  1:105636/1‑149 (MQ=255)
                                                                                     tCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATg                                                           <  7:421277/148‑1 (MQ=255)
                                                                                             tgtTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTtttattttat                                                                                 <  2:242886/118‑1 (MQ=255)
                                                                                               ttttCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGCCGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGt                                                >  1:286478/1‑149 (MQ=255)
                                                                                               ttttCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGAATATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTAAACATCTACTGGTAAATGCAAGTCGTAGt                                                >  3:414785/1‑149 (MQ=255)
                                                                                                   cACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATg                                                                                                                                 >  6:302082/1‑64 (MQ=255)
                                                                                                        cttGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGaa                                        >  3:98240/1‑148 (MQ=255)
                                                                                                           gATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCtttt                                                                                        >  8:6214/1‑97 (MQ=255)
                                                                                                           gATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCtttt                                                                                        <  7:6214/97‑1 (MQ=255)
                                                                                                              atataACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttattt                                                                                    <  8:86349/98‑1 (MQ=255)
                                                                                                              atataACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttattt                                                                                    >  7:86349/1‑98 (MQ=255)
                                                                                                                 taACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCt                              >  1:219629/1‑149 (MQ=255)
                                                                                                                   aCTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCa                            <  2:139357/149‑1 (MQ=255)
                                                                                                                   aCTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCa                            >  4:384096/1‑149 (MQ=255)
                                                                                                                     ttttATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTAc                                                                    >  3:97410/1‑107 (MQ=255)
                                                                                                                     ttttATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTAc                                                                    >  6:25685/1‑107 (MQ=255)
                                                                                                                     ttttATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCAc                           >  5:33026/1‑148 (MQ=255)
                                                                                                                     ttttATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCAc                           >  6:355913/1‑148 (MQ=255)
                                                                                                                       ttATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCa                         <  8:371558/148‑1 (MQ=255)
                                                                                                                        tATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCAt                        <  8:406331/148‑1 (MQ=255)
                                                                                                                            tGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAat                    <  2:326765/148‑1 (MQ=255)
                                                                                                                              cTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATg                 >  3:201174/1‑149 (MQ=255)
                                                                                                                               tCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGc                >  6:165011/1‑149 (MQ=255)
                                                                                                                                 aaaCGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCtt              <  7:439796/149‑1 (MQ=255)
                                                                                                                                 aaaCGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCtt              <  3:231828/149‑1 (MQ=255)
                                                                                                                                  aaCGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCtt              >  3:324656/1‑148 (MQ=255)
                                                                                                                                  aaCGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCtt              <  4:324656/148‑1 (MQ=255)
                                                                                                                                    cGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAATATTTACCCTCACCATAAATATGCTTCAg           >  1:167547/1‑149 (MQ=255)
                                                                                                                                    cGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAg           >  7:408180/1‑149 (MQ=255)
                                                                                                                                        aGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTAtt       <  3:104963/149‑1 (MQ=255)
                                                                                                                                        aGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTAtt       <  1:229221/149‑1 (MQ=255)
                                                                                                                                         gACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTAttt      <  5:355913/149‑1 (MQ=255)
                                                                                                                                         gACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTAttt      <  2:412380/149‑1 (MQ=255)
                                                                                                                                            ggCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTa   <  8:251911/149‑1 (MQ=255)
                                                                                                                                            ggCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTa   <  8:245648/149‑1 (MQ=255)
                                                                                                                                             gCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCCAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTAt  <  1:151517/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTAT  >  NC_000913/3798506‑3798796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: