BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,413,461	G→A	intergenic (‑329/‑9)	yfbV ← / → ackA	UPF0208 family inner membrane protein/acetate kinase A and propionate kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,413,461	0	G	A	100.0%	472.9 / NA	131	intergenic (‑329/‑9)	yfbV/ackA	UPF0208 family inner membrane protein/acetate kinase A and propionate kinase 2
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (66/65); total (66/65)

Only 100 of 131 total aligned reads displayed.

CGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAAT  >  NC_000913/2413320‑2413605

cGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACtt                                                                                                                                            <  6:395493/148‑1 (MQ=255)

 gCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                          <  4:316708/149‑1 (MQ=255)

 gCAAAATGGCATAGACTAAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                          <  7:47106/149‑1 (MQ=255)

  cAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCa                                                                                                                                         >  1:42668/1‑149 (MQ=255)

   aaaaTGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCAt                                                                                                                                        <  2:33509/149‑1 (MQ=255)

   aaaaTGGCATAGACTCAAGATATTTCTTCCATCATGC‑AAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATg                                                                                                                                       >  2:357513/1‑149 (MQ=255)

      aTGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCTTAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTc                                                                                                                                     <  3:180890/149‑1 (MQ=255)

         gCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAg                                                                                                                                  <  6:433757/149‑1 (MQ=255)

            tAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTaa                                                                                                                               <  5:337547/149‑1 (MQ=255)

               aCTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGt                                                                                                                             >  1:283634/1‑148 (MQ=255)

               aCTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGt                                                                                                                             <  1:110126/148‑1 (MQ=255)

                cTCAAGATATTTCTTCCATCCTGCAAAAAAAATTTGCAGTGCATGAAGTTAATCATAAATGTCGGGGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTATCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTa                                                                                                                           <  6:59458/149‑1 (MQ=255)

                 tCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAg                                                                                                                          >  4:266343/1‑149 (MQ=255)

                 tCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAA‑TATAAGTACTTCCATGTCGAGTAAGTTAg                                                                                                                          >  6:202377/1‑148 (MQ=255)

                   aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACt                                                                                                                                             <  4:148102/128‑1 (MQ=255)

                   aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGt                                                                                                                         >  2:416880/1‑148 (MQ=255)

                   aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTa                                                                                                                        >  4:311406/1‑149 (MQ=255)

                   aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTa                                                                                                                        <  3:378082/149‑1 (MQ=255)

                   aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTa                                                                                                                        >  3:368662/1‑149 (MQ=255)

                   aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATAATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTc                                                                                                                                           <  7:327175/130‑1 (MQ=255)

                   aaGATAAATCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTAGGTGTCCTCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGACACGTATCAATTAGAAGTACTGCCATGACGACTAAGATAgaa                                                                                                                        >  8:401270/1‑147 (MQ=255)

                    aGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTAc                                                                                                                       >  1:117961/1‑149 (MQ=255)

                         tttCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGt                                                                                                                   >  1:213260/1‑148 (MQ=255)

                           tcttcCATCATGC‑AAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCt                                                                                                                <  2:421521/148‑1 (MQ=255)

                            cttcCCTCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCCTCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCt                                                                                                                <  8:320940/148‑1 (MQ=255)

                               ccATCATGCAAAAAAAAATTGCAGTGCATGATGTTAATCAAAAATGTCGGTGTCATCATGCGCTAAGCTCTATGGATCCCTGACGTTGTTTTAGCCACGTATCAATTATAAGTACTTGACTGTGTAGTAAGATAGTACTTCTTCTGAAc                                                                                                            >  2:61756/1‑149 (MQ=255)

                                 atcatGCATAAAATATTTGCAGTGCATGATGTGAATCATAAATGTCGGTGTCATCATGCGCTACGCTGTATGGCTCCGTGACGGTTTTATAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGATCTgcagtg                                                                                                          >  1:227436/1‑144 (MQ=255)

                                  tcatGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGc                                                                                                         >  7:241968/1‑149 (MQ=255)

                                   catGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCg                                                                                                        >  6:149844/1‑149 (MQ=255)

                                     tGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGt                                                                                                      <  2:283634/149‑1 (MQ=255)

                                        aaaaaaaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGt                                                                                                   >  8:329279/1‑149 (MQ=255)

                                        aaaaaaaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGt                                                                                                   >  3:147153/1‑149 (MQ=255)

                                        aaaaaaaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTAGAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGCACTGCGGTAGt                                                                                                   >  6:387825/1‑149 (MQ=255)

                                         aaaaaaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGtt                                                                                                  >  2:375146/1‑149 (MQ=255)

                                         aaaaaaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGtt                                                                                                  <  3:311406/149‑1 (MQ=255)

                                         aaaaaaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGAGGTAGtt                                                                                                  >  7:312219/1‑149 (MQ=255)

                                            aaaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCgg                                                                                                       <  5:67413/141‑1 (MQ=255)

                                            aaaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCgg                                                                                                       >  6:67413/1‑141 (MQ=255)

                                            aaaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGttctt                                                                                               <  2:117961/149‑1 (MQ=255)

                                            aaaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGttctt                                                                                               <  3:229467/149‑1 (MQ=255)

                                             aaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTGCTGAACTGCGGTAGttcttc                                                                                              <  2:230612/149‑1 (MQ=255)

                                                  tGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTg                                                                                          <  5:295709/148‑1 (MQ=255)

                                                     aGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCCGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTa                                                                                                                        >  3:245085/1‑115 (MQ=255)

                                                     aGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCCGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTa                                                                                                                        <  4:245085/115‑1 (MQ=255)

                                                      gTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAtt                                                                                     >  2:321130/1‑149 (MQ=255)

                                                         cATGATGTTAATCATAAATGTCGGTGTCATCAAGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGc                                                                                  <  4:381619/149‑1 (MQ=255)

                                                          atgatgTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGc                                                                                  >  5:107124/1‑148 (MQ=255)

                                                            gatgTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCcat                                                                               >  1:321779/1‑149 (MQ=255)

                                                                   aTCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAttt                                                                                    <  6:364662/137‑1 (MQ=255)

                                                                   aTCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAttt                                                                                    >  5:364662/1‑137 (MQ=255)

                                                                   aTCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGa                                                                         <  3:113441/148‑1 (MQ=255)

                                                                   aTCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGAt                                                                        >  2:420387/1‑149 (MQ=255)

                                                                      aTAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCa                                                                     <  1:250630/149‑1 (MQ=255)

                                                                       tAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCgg                                                                                                       <  4:321039/114‑1 (MQ=255)

                                                                       tAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCgg                                                                                                       >  3:321039/1‑114 (MQ=255)

                                                                        aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAttt                                                                                    <  1:208780/132‑1 (MQ=255)

                                                                        aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAg                                                                    >  4:265006/1‑148 (MQ=255)

                                                                          aTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTa                                                                  >  1:18629/1‑148 (MQ=255)

                                                                             tCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAAt                                                               <  8:39387/148‑1 (MQ=255)

                                                                             tCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATg                                                              <  1:423288/149‑1 (MQ=255)

                                                                               ggTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGt                                                            <  7:67294/149‑1 (MQ=255)

                                                                               ggTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGt                                                            <  1:357513/149‑1 (MQ=255)

                                                                                gtgtCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGt                                                            >  4:259244/1‑148 (MQ=255)

                                                                                 tgtCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTg                                                           >  6:382536/1‑148 (MQ=255)

                                                                                 tgtCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAA‑TATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGaa                                                         <  5:202377/149‑1 (MQ=255)

                                                                                     atcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAagag                                                      >  5:106477/1‑149 (MQ=255)

                                                                                     atcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAAATGCGGTAGTTCTTAACTGAAAATTGCCATCATCGATGCAGTAAATGGTGAagat                                                      >  6:112630/1‑148 (MQ=255)

                                                                                       catGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTa                                                    >  3:36876/1‑149 (MQ=255)

                                                                                        atGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTa                                                    <  5:149844/148‑1 (MQ=255)

                                                                                        atGCGATTCGCTCTTTGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAATAGTAc                                                   <  8:312219/149‑1 (MQ=255)

                                                                                          gcgcTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCt                                                 >  7:4268/1‑149 (MQ=255)

                                                                                              tACGCTCTATGGCTACCTGACGTTTTTTTAGCCACGTATAAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAAATGCGTTAGTTCTTCACTGAAATTTGCAATCATCTATGAAGTAAATGGTGAAGAGTAACTTTCt                                             >  5:96032/1‑149 (MQ=255)

                                                                                                cGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCt                                             <  1:416880/147‑1 (MQ=255)

                                                                                                cGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTg                                            <  2:42668/148‑1 (MQ=255)

                                                                                                cGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATAAATTATAAGTACTTCCATTTCGAGTAATTTAGTTCTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCtt                                            <  2:292574/148‑2 (MQ=255)

                                                                                                 gCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGt                                          <  8:241968/149‑1 (MQ=255)

                                                                                                   tctATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGtt                                         >  7:9148/1‑148 (MQ=255)

                                                                                                   tctATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGtt                                         >  7:240075/1‑148 (MQ=255)

                                                                                                     tATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAg                                      >  4:146618/1‑149 (MQ=255)

                                                                                                       tGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTa                                       <  6:107124/146‑1 (MQ=255)

                                                                                                       tGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTa                                       <  1:375146/146‑1 (MQ=255)

                                                                                                       tGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGc                                     <  5:425503/148‑1 (MQ=255)

                                                                                                         gCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCCTTCTGGTTTAGCCg                                   >  4:143036/1‑148 (MQ=255)

                                                                                                             ccTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTa                                       >  7:180525/1‑140 (MQ=255)

                                                                                                             ccTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTa                                       <  8:180525/140‑1 (MQ=255)

                                                                                                                gACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGttctt                                                                                               <  7:152688/81‑1 (MQ=255)

                                                                                                                 aCGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTc                           <  1:47969/148‑1 (MQ=255)

                                                                                                                    tttttttAGCCACGTATCAATTATAAGTAATTCCATGTAGAGTAAGTTAGTACTGGTTCTGAACTGAGGTAGTTCTTCACTGAAATTTGACATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCTAATGTTTCCAcc                       >  6:182856/1‑149 (MQ=255)

                                                                                                                        tttAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGcc                   >  2:210020/1‑149 (MQ=255)

                                                                                                                        tttAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGcc                   >  4:397094/1‑149 (MQ=255)

                                                                                                                        tttAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGc                    <  3:326554/148‑1 (MQ=255)

                                                                                                                        tttAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGc                    <  8:240075/148‑1 (MQ=255)

                                                                                                                           aGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTg                                            <  8:230966/121‑1 (MQ=255)

                                                                                                                           aGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTg                                            >  7:230966/1‑121 (MQ=255)

                                                                                                                             acACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGAGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAg              <  5:387825/148‑1 (MQ=255)

                                                                                                                                cGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCAc           >  7:161303/1‑149 (MQ=255)

                                                                                                                                cGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCAc           <  1:321130/149‑1 (MQ=255)

                                                                                                                                    tCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGACGCACGTAt       >  4:3905/1‑149 (MQ=255)

                                                                                                                                      aaTTATAAGTACTTCCATGTCGAGTAAGTTAGTAATGGTTCTGAACTGCTGTAGTTCTTCACTGAAATTTGCCATCATGGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCAGGTATc      <  2:227436/148‑1 (MQ=255)

                                                                                                                                         tataAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCaaat  >  8:276829/1‑149 (MQ=255)

CGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAAT  >  NC_000913/2413320‑2413605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`