Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,383,685	C→A	E93* (GAA→TAA)	ycjW ←	LacI family putative transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,383,685	0	C	A	100.0%	117.0 / NA	34	E93* (GAA→TAA)	ycjW	LacI family putative transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/17);  total (17/17)

TCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTT  >  NC_000913/1383540‑1383818                                                                                                                                                  |                                                                                                                                      tCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACcttcttatt                                                                                                                                     <  5:622/148‑1 (MQ=255)            aaaaaaTGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCgg                                                                                                                         >  6:26141/1‑149 (MQ=255)                aaTGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGTGGTGAt                                                                                                                     <  6:128194/149‑1 (MQ=255)                      tCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATg                                                                                                               >  1:93868/1‑149 (MQ=255)                      tCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATg                                                                                                               >  8:17240/1‑149 (MQ=255)                        aTCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGc                                                                                                              <  8:7464/148‑1 (MQ=255)                                gCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCa                                                                                                     <  5:7419/149‑1 (MQ=255)                                    aCTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCaaaa                                                                                                          >  5:111457/1‑140 (MQ=255)                                    aCTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCaaaa                                                                                                          <  6:111457/140‑1 (MQ=255)                                        aGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCa                                                                                                                 >  6:63239/1‑129 (MQ=255)                                        aGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCa                                                                                                                 <  5:63239/129‑1 (MQ=255)                                           ataataATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACg                                                                                           >  4:118018/1‑148 (MQ=255)                                             aataatGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTc                                                                                                      <  1:11513/135‑1 (MQ=255)                                             aataatGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTc                                                                                                      >  2:11513/1‑135 (MQ=255)                                              ataatGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGAt                                                                                        <  7:17240/148‑1 (MQ=255)                                                          aTCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACg                                                                                           <  3:72239/133‑1 (MQ=255)                                                          aTCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACg                                                                                           >  4:72239/1‑133 (MQ=255)                                                                                tttCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAAt                                                      >  7:72229/1‑148 (MQ=255)                                                                                   cGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAc                                                   <  8:72229/148‑1 (MQ=255)                                                                                    gCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTa                                                           <  4:110177/139‑1 (MQ=255)                                                                                    gCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTa                                                           >  3:110177/1‑139 (MQ=255)                                                                                         tttGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAAt                                             <  2:93868/148‑1 (MQ=255)                                                                                             cAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATg                                            <  8:47633/145‑1 (MQ=255)                                                                                             cAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATg                                            >  7:47633/1‑145 (MQ=255)                                                                                              aGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCAt                                        >  7:66179/1‑148 (MQ=255)                                                                                                aTCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTc                                                                    <  4:67698/118‑1 (MQ=255)                                                                                                aTCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTc                                                                    >  3:67698/1‑118 (MQ=255)                                                                                                aTCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACggg                                                             <  1:21501/125‑1 (MQ=255)                                                                                                aTCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACggg                                                             >  2:21501/1‑125 (MQ=255)                                                                                                aTCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCa                                      >  7:35028/1‑148 (MQ=255)                                                                                                     ctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAccc                                >  2:9376/1‑149 (MQ=255)                                                                                                             ttGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGt                        <  3:118018/149‑1 (MQ=255)                                                                                                                                   cGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGttt  <  8:66179/149‑1 (MQ=255)                                                                                                                                         aaCCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGGTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGt    <  3:117673/141‑1 (MQ=255)                                                                                                                                         aaCCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGGTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGt    >  4:117673/1‑141 (MQ=255)                                                                                                                                                  |                                                                                                                                      TCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTT  >  NC_000913/1383540‑1383818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑