Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,287,423 G→C intergenic (‑52/‑341) acs ← / → nrfA acetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,287,4230GC99.0% 362.3 / NA 101intergenic (‑52/‑341)acs/nrfAacetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (54/45);  minor base A (1/0);  total (55/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

ATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTT  >  NC_000913/4287277‑4287567
                                                                                                                                                  |                                                                                                                                                
aTAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGATTTTGGCTCATGCTTTTTTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTa                                                                                                                                                >  5:302000/1‑149 (MQ=255)
   gcttgttgATAGAGCGCCTCGTACTGCTGACGGTTTATCAGGCCAAGGTGTGCGATGTTGGCAGGAATGGTGTGTGTGTTAATTTGGCTCATGCTTTTTTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAgg                                                                                                                                              <  8:286141/146‑1 (MQ=255)
   gATTGTTGATACATCGCCTAGTACTGCTGATGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAgg                                                                                                                                              <  2:137310/148‑1 (MQ=255)
    aTTGTTGATACATCGGCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAggg                                                                                                                                             <  2:224573/148‑1 (MQ=255)
    aTTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAgggg                                                                                                                                            >  3:21018/1‑149 (MQ=255)
     ttgttgATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAgggg                                                                                                                                            >  1:296181/1‑148 (MQ=255)
        ttgATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTAtt                                                                                                                                        >  4:151951/1‑149 (MQ=255)
          gATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTgg                                                                                                                                      >  7:294304/1‑149 (MQ=255)
              cATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGt                                                                                                                                  <  1:355671/149‑1 (MQ=255)
               aTCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGtt                                                                                                                                 >  2:235136/1‑149 (MQ=255)
               aTCGCCTCGGACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTATCCTTGGAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGtt                                                                                                                                 >  8:358135/1‑149 (MQ=255)
                   ccTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGTGTATTGGCAGTTTTGt                                                                                                                             >  1:296390/1‑149 (MQ=255)
                    cTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGtt                                                                                                                            <  4:21018/149‑1 (MQ=255)
                       gTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTa                                                                                                                          <  1:85500/148‑1 (MQ=255)
                       gTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTa                                                                                                                          <  7:354157/148‑1 (MQ=255)
                           tgctgAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTa                                                                                                                          >  5:263462/1‑144 (MQ=255)
                           tgctgAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTa                                                                                                                          <  6:263462/144‑1 (MQ=255)
                             ctgAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTAttt                                                                                                                    <  3:155335/148‑1 (MQ=255)
                             ctgAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTg                                                                                                                   >  5:151364/1‑149 (MQ=255)
                              tgAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTgg                                                                                                                  >  3:43117/1‑149 (MQ=255)
                               gAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTggg                                                                                                                 >  2:139572/1‑149 (MQ=255)
                                aGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTa                                                                                                                          <  2:322254/139‑1 (MQ=255)
                                aGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTa                                                                                                                          >  1:322254/1‑139 (MQ=255)
                                aGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGc                                                                                                                >  5:331791/1‑149 (MQ=255)
                                  ggTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGa                                                                                                              >  7:305045/1‑149 (MQ=255)
                                  ggTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGa                                                                                                              >  2:146537/1‑149 (MQ=255)
                                  ggTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGa                                                                                                              >  8:47711/1‑149 (MQ=255)
                                   gTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTTTTTGTGAATTTGGCTGATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGAc                                                                                                             <  2:234404/149‑1 (MQ=255)
                                   gTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGAc                                                                                                             <  2:296181/149‑1 (MQ=255)
                                    tttATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTTAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGtt                                                                                                                            >  5:254247/1‑133 (MQ=255)
                                    tttATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGtt                                                                                                                            <  6:254247/133‑1 (MQ=255)
                                    tttATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGa                                                                                                              >  6:13723/1‑147 (MQ=255)
                                    tttATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACa                                                                                                            <  3:235821/149‑1 (MQ=255)
                                       aTCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGa                                                                                                              >  6:137599/1‑144 (MQ=255)
                                        tCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAt                                                                                                         <  1:235136/148‑1 (MQ=255)
                                          aGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCAc                                                                                                      >  4:232566/1‑149 (MQ=255)
                                          aGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACg                                                                                                     >  3:97218/1‑149 (MQ=255)
                                           ggCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACg                                                                                                     >  7:253265/1‑149 (MQ=255)
                                              aaCGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCa                                                                                                   >  7:377760/1‑148 (MQ=255)
                                                 ggTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAg                                                                                               >  2:17316/1‑149 (MQ=255)
                                                 ggTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAg                                                                                               <  5:303064/149‑1 (MQ=255)
                                                   tCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTa                                                                                             <  3:151951/149‑1 (MQ=255)
                                                     tGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAg                                                                                            >  5:270807/1‑148 (MQ=255)
                                                      gCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAgg                                                                                                                                              >  5:199622/1‑97 (MQ=255)
                                                      gCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAgg                                                                                                                                              <  6:199622/97‑1 (MQ=255)
                                                       cGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGaa                                                                                          <  5:13723/148‑1 (MQ=255)
                                                         aTGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATtg                                                                                       >  6:110765/1‑149 (MQ=255)
                                                            ttGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGc                                                                                    <  4:97218/148‑1 (MQ=255)
                                                             tGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAATTGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGc                                                                                    <  7:97280/148‑1 (MQ=255)
                                                                  ggAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATa                                                                               >  5:50239/1‑148 (MQ=255)
                                                                  ggAATGGTGTGTTTGTGAATTTCGCTCATGCTTTTGATCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATa                                                                               >  4:330318/1‑148 (MQ=255)
                                                                        gtgtgtTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCa                                                                        <  3:232566/149‑1 (MQ=255)
                                                                           tgtTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAggg                                                                     >  6:176358/1‑149 (MQ=255)
                                                                                gtgAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAtt                                                                >  3:343685/1‑149 (MQ=255)
                                                                                gtgAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAtt                                                                >  3:231455/1‑149 (MQ=255)
                                                                                  gAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGGTAAATCTAGGGGTATTGGCAGTTATGTTTAGTATTTGGGCGACAGATCACGCAAAAGTATAATTGTGCAAATAAACTGCAGGGTAAtttt                                                              >  8:303801/1‑149 (MQ=255)
                                                                                   aaTTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAttttt                                                             <  4:231455/149‑1 (MQ=255)
                                                                                     tttGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTg                                                            >  8:296181/1‑148 (MQ=255)
                                                                                       tGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGaa                                                          <  6:331791/148‑1 (MQ=255)
                                                                                        ggcgcctGCTTTTGTTCTCCTTGTAGGCTTGTAATAATATGTGGCATACGCGTTAACTCTAGGGGGATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAg                                                         <  5:374988/142‑1 (MQ=255)
                                                                                         gCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGt                                                       <  8:294304/149‑1 (MQ=255)
                                                                                             aTGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATATAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAg                                                    >  3:116699/1‑148 (MQ=255)
                                                                                                         tcCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGGTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGa                                       >  2:221277/1‑149 (MQ=255)
                                                                                                           cTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTg                                        <  8:253265/146‑1 (MQ=255)
                                                                                                           cTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGAtt                                     <  7:47711/149‑1 (MQ=255)
                                                                                                           cTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGAt                                      <  6:151364/148‑1 (MQ=255)
                                                                                                            ttGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGAtt                                     >  5:119846/1‑148 (MQ=255)
                                                                                                              gTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAAt                                  >  6:12560/1‑149 (MQ=255)
                                                                                                              gTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAAt                                  >  1:391978/1‑149 (MQ=255)
                                                                                                               tAGGATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAAtt                                 <  7:8746/149‑1 (MQ=255)
                                                                                                                 ggATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCt                               <  4:343685/149‑1 (MQ=255)
                                                                                                                  gATGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCtt                              >  5:71602/1‑149 (MQ=255)
                                                                                                                   aTGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                             <  3:330318/149‑1 (MQ=255)
                                                                                                                   aTGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                             <  7:358135/149‑1 (MQ=255)
                                                                                                                    tGTTAATTATATGTGGTATACGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTTGAATTGTTCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAACGTTGATTAATTCTTTg                            <  6:302000/149‑1 (MQ=255)
                                                                                                                    tGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTg                            <  4:3100/149‑1 (MQ=255)
                                                                                                                    tGTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTg                            <  7:65678/149‑1 (MQ=255)
                                                                                                                     gTTAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                             >  1:205636/1‑147 (MQ=255)
                                                                                                                      ttAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGAt                                      <  3:53726/137‑1 (MQ=255)
                                                                                                                      ttAATAATATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGAt                                      >  4:53726/1‑137 (MQ=255)
                                                                                                                        aataatATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGt                                          <  6:268872/131‑1 (MQ=255)
                                                                                                                        aataatATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGt                                          >  5:268872/1‑131 (MQ=255)
                                                                                                                        aataatATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGa                           <  6:212736/146‑1 (MQ=255)
                                                                                                                        aataatATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGa                           >  5:212736/1‑146 (MQ=255)
                                                                                                                        aataatATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAgg                         >  6:41497/1‑148 (MQ=255)
                                                                                                                        aataatATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAgg                         >  8:60810/1‑148 (MQ=255)
                                                                                                                        aataatATGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAgg                         <  5:75240/148‑1 (MQ=255)
                                                                                                                         ataataTGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGa                        <  1:17316/148‑1 (MQ=255)
                                                                                                                            atatGTGGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATACTTTg                            <  7:95803/141‑1 (MQ=255)
                                                                                                                               tgtgGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATg                   >  2:56298/1‑147 (MQ=255)
                                                                                                                                gtgGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAg                >  2:261882/1‑149 (MQ=255)
                                                                                                                                 tgGCATTAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAATATTAGAATTGTGCAATTAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGTAACATGCAg                <  7:6657/148‑1 (MQ=255)
                                                                                                                                 tgGCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGt               >  6:150748/1‑149 (MQ=255)
                                                                                                                                   gCATAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGtt              <  5:12560/148‑1 (MQ=255)
                                                                                                                                     aTAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAtttt                                                              <  8:194107/98‑1 (MQ=255)
                                                                                                                                     aTAAGCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAtttt                                                              >  7:194107/1‑98 (MQ=255)
                                                                                                                                         gCGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTatgcatg       >  6:194975/1‑149 (MQ=255)
                                                                                                                                          cGTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTatgcatgc      <  7:296181/149‑1 (MQ=255)
                                                                                                                                           gTTAAATCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCt     >  3:231447/1‑149 (MQ=255)
                                                                                                                                               aaTCTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGtt  >  2:285977/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTT  >  NC_000913/4287277‑4287567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: