Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,159,109 A→G N414S (AAC→AGC)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,159,1090AG99.1% 394.0 / ‑4.6 109N414S (AAC→AGC) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (58/50);  total (59/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 111 total aligned reads displayed.

GATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACT  >  NC_000913/1158963‑1159251
                                                                                                                                                  |                                                                                                                                              
gATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACTCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCa                                                                                                                                              >  2:48080/1‑149 (MQ=255)
gATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCa                                                                                                                                              >  5:198754/1‑149 (MQ=255)
gATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCa                                                                                                                                              >  4:7843/1‑149 (MQ=255)
 aTTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCAt                                                                                                                                             <  1:384932/149‑1 (MQ=255)
    gTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTa                                                                                                                                           <  7:110727/148‑1 (MQ=255)
     tttACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTAc                                                                                                                                          >  3:74119/1‑148 (MQ=255)
       tactacACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTaa                                                                                                                                       >  2:222666/1‑149 (MQ=255)
        actacACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAAc                                                                                                                                      >  7:21904/1‑149 (MQ=255)
         ctacACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGCCGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGGATTTGGTGGTAAAGAAAGCATTACTAAcc                                                                                                                                     >  5:296447/1‑149 (MQ=255)
          tacACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCt                                                                                                                                    >  6:221681/1‑149 (MQ=255)
            cacCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACCCCGCGACGTGAAGACGCGACTCAAGATGCAAAAACGACAGGTACCACCGAAATGACACCGGCTCAGGTTGCTGCATTTGTTGGTAAAGAAAGCATTACTAAccag                                                                                                                                   >  4:16746/1‑146 (MQ=255)
             acCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGa                                                                                                                                 >  1:90152/1‑149 (MQ=255)
                  cTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCAt                                                                                                                            >  1:196168/1‑149 (MQ=255)
                     ccGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGt                                                                                                                          <  8:11824/148‑1 (MQ=255)
                      cgcgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTa                                                                                                                         <  3:148772/148‑1 (MQ=255)
                      cgcgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTa                                                                                                                         <  8:333670/148‑1 (MQ=255)
                      agctttgtGATTAAAGCACTGGATCTGACAACGCCGTGTCGTGAAGACGCGACTGAAGATGCAAAATCGACAGGTACCAGCGAAATGGCACCGTCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTAt                                                                                                                        <  5:244044/142‑1 (MQ=255)
                       gcgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTAtt                                                                                                                       <  5:221681/149‑1 (MQ=255)
                         gTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTAc                                                                                                                     <  4:254037/149‑1 (MQ=255)
                            cTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTAccc                                                                                                                   <  7:277613/148‑1 (MQ=255)
                              gATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCATCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTc                                                                                                                >  5:352079/1‑149 (MQ=255)
                              gATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTc                                                                                                                >  6:353444/1‑149 (MQ=255)
                                   aaGCACTGGATCTGAAAACGCCGGGTCGTGAAGACACGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTgc                                                                                                             <  2:44675/147‑1 (MQ=255)
                                   aaGCACTGGATCTGAAAACGCCGGGTCGTGAAGACACGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTgc                                                                                                             >  1:44675/1‑147 (MQ=255)
                                    aGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTa                                                                                                                                           <  4:345285/116‑1 (MQ=255)
                                    aGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTa                                                                                                                                           >  3:345285/1‑116 (MQ=255)
                                    aGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGcgcg                                                                                                          >  6:257318/1‑149 (MQ=255)
                                    aGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGcgcg                                                                                                          <  2:42591/149‑1 (MQ=255)
                                    aGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGcgcg                                                                                                          >  2:232416/1‑149 (MQ=255)
                                        cTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAg                                                                                                      >  4:173337/1‑149 (MQ=255)
                                         tGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTAccc                                                                                                                   >  7:10689/1‑135 (MQ=255)
                                         tGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTAccc                                                                                                                   <  8:10689/135‑1 (MQ=255)
                                           gATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGt                                                                                                   >  2:13040/1‑149 (MQ=255)
                                               tGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCt                                                                                               >  6:108133/1‑149 (MQ=255)
                                                 aaaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGa                                                                                             <  7:374522/149‑1 (MQ=255)
                                                 aaaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGa                                                                                             <  6:316000/149‑1 (MQ=255)
                                                  aaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCATCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                                                                            <  6:352079/149‑1 (MQ=255)
                                                  aaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                                                                            >  3:338852/1‑149 (MQ=255)
                                                    aCGCCGGGTCGTGAAGACGCGTCTGAAGATGCAAAAGCGACAGGTACCAGCGAAATAGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAtgt                                                                                          <  6:364774/149‑1 (MQ=255)
                                                    aCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAtgt                                                                                          <  6:178474/149‑1 (MQ=255)
                                                       ccGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTc                                                                                       <  6:341097/149‑1 (MQ=255)
                                                       ccGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTc                                                                                       >  6:236334/1‑149 (MQ=255)
                                                         gggTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                                                                            <  5:330283/142‑1 (MQ=255)
                                                         gggTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                                                                            >  6:330283/1‑142 (MQ=255)
                                                              gTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGt                                                                                 <  1:13040/148‑1 (MQ=255)
                                                               tGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTgg                                                                               <  7:134306/149‑1 (MQ=255)
                                                                 aaGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATgtgt                                                                                        >  2:356747/1‑138 (MQ=255)
                                                                 aaGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATgtgt                                                                                        <  1:356747/138‑1 (MQ=255)
                                                                 aaGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAt                                                                             <  6:198754/149‑1 (MQ=255)
                                                                  ataCGCGTCTGAAGTTGCAAAAGCGACAGGTAACAGCGAAATGGCACCGGCTCTTGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGAGTCAGCGTTGCTGATGTGTCTAAAGTGGATc                                                                            <  2:90152/147‑1 (MQ=255)
                                                                   gACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCAGTTGGTGGTAAAGAAAGCATTACTAACATCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAGGTGTCTAAAGTGGAt                                                                             >  6:253929/1‑147 (MQ=255)
                                                                    aCGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAg                                                                          <  3:173337/149‑1 (MQ=255)
                                                                        gACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccg                                                                      >  8:15413/1‑149 (MQ=255)
                                                                        gACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccg                                                                      >  4:17781/1‑149 (MQ=255)
                                                                           tGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccggcc                                                                   >  5:337425/1‑149 (MQ=255)
                                                                            gAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCt                                                                  >  5:168253/1‑149 (MQ=255)
                                                                             aaGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTACCCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCt                                                                  >  4:130564/1‑148 (MQ=255)
                                                                             aaGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCt                                                                  >  3:64703/1‑148 (MQ=255)
                                                                                 tGCAAAAGCGACATGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTAGACGCATGTATTACCCGTCTGCGCGTCAGCGTAGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTgaag                                                              >  5:306759/1‑148 (MQ=255)
                                                                                     aaaGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACt                                                         >  2:123113/1‑149 (MQ=255)
                                                                                       aGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccggc                                                                    >  5:224913/1‑136 (MQ=255)
                                                                                       aGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccggc                                                                    <  6:224913/136‑1 (MQ=255)
                                                                                        gCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTggg                                                      <  2:280214/149‑1 (MQ=255)
                                                                                        gCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTggg                                                      <  4:74119/149‑1 (MQ=255)
                                                                                            cAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGgcg                                                    >  2:123252/1‑147 (MQ=255)
                                                                                            cAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCa                                                  <  7:15413/149‑1 (MQ=255)
                                                                                             aGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGTGCTCAg                                                 >  2:169520/1‑149 (MQ=255)
                                                                                             aGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAg                                                 >  6:338109/1‑149 (MQ=255)
                                                                                                   cAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAATAAAGCATTACTAACCTCGAATCATGTATTACCCGTATTCGAGTCAGCGTTGCTGATGTGTCTAAATTTGATCAGGCAGGCCTGAAGAAAATGTGCGAATCGGGCg                                           >  7:209970/1‑149 (MQ=255)
                                                                                                   cAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCg                                           >  4:256560/1‑149 (MQ=255)
                                                                                                    aGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCg                                           >  7:204515/1‑148 (MQ=255)
                                                                                                     gCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTa                                         <  1:224768/149‑1 (MQ=255)
                                                                                                     gCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCGGAAGAAACTGGGCGCAGCGGGCGTa                                         <  5:353444/149‑1 (MQ=255)
                                                                                                     gCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAAACTAGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCATGCCGGCCTGAAGAAACTGGGCGCACCGGGCg                                           >  1:94587/1‑147 (MQ=255)
                                                                                                       gAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAg                                                                          <  8:290848/114‑1 (MQ=255)
                                                                                                        aaaTGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTg                                      <  3:7843/149‑1 (MQ=255)
                                                                                                             gCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGc                                 <  3:17781/149‑1 (MQ=255)
                                                                                                              cACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCt                                <  8:21904/149‑1 (MQ=255)
                                                                                                              cACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCt                                <  2:196168/149‑1 (MQ=255)
                                                                                                               aCCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTg                               >  3:135280/1‑149 (MQ=255)
                                                                                                                ccGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTACCCTCGACGCATGTATTACCCGTCTGCGCGGCAGCGGTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTgg                              <  5:257318/149‑1 (MQ=255)
                                                                                                                 cGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTa                                                                                                                         <  5:100587/57‑1 (MQ=255)
                                                                                                                 cGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTa                                                                                                                         >  6:100587/1‑57 (MQ=255)
                                                                                                                   gCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTc                           >  1:182134/1‑149 (MQ=255)
                                                                                                                       tGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTgaa                                                               >  4:117585/1‑109 (MQ=255)
                                                                                                                       tGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGgt                       >  3:65838/1‑149 (MQ=255)
                                                                                                                         gTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGgtgt                     >  3:98507/1‑149 (MQ=255)
                                                                                                                          ttGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGtt                    >  4:65885/1‑149 (MQ=255)
                                                                                                                            gctgcATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTc                   <  1:222666/148‑1 (MQ=255)
                                                                                                                             ctgcATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAg                 <  7:71337/149‑1 (MQ=255)
                                                                                                                              tgcATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCa                  >  5:29444/1‑147 (MQ=255)
                                                                                                                              tgcATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCa                  <  6:29444/147‑1 (MQ=255)
                                                                                                                              tgcATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAgg                >  1:362435/1‑149 (MQ=255)
                                                                                                                                     ggtggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGAtttt         >  2:145039/1‑149 (MQ=255)
                                                                                                                                     ggtggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGAttt          <  7:86991/148‑1 (MQ=255)
                                                                                                                                      gtggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGAtttt         <  6:337425/148‑1 (MQ=255)
                                                                                                                                         gtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGCAGAAACTGGGCGCAGCGGGCGTAGTGGTGGCTGGTTCTGGTGTTCAGGCGATTTTCgg      >  6:297904/1‑148 (MQ=255)
                                                                                                                                            aaGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACt  <  1:326181/149‑1 (MQ=255)
                                                                                                                                               aaagCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTg                         >  4:251436/1‑123 (MQ=255)
                                                                                                                                               aaagCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTg                         <  3:251436/123‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACT  >  NC_000913/1158963‑1159251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: