Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,383,685	C→A	E93* (GAA→TAA)	ycjW ←	LacI family putative transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,383,685	0	C	A	100.0%	221.4 / NA	64	E93* (GAA→TAA)	ycjW	LacI family putative transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/38);  total (26/38)

TCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACT  >  NC_000913/1383540‑1383828                                                                                                                                                  |                                                                                                                                                tCGAGTTGGGCAAAAAATGCCTCATACGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACATTTGTAATATCACGTCGAAACCttcttattc                                                                                                                                              <  4:18115/149‑1 (MQ=255) tCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACcttcttatt                                                                                                                                               <  2:165973/148‑1 (MQ=255) tCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACcttcttatt                                                                                                                                               <  8:122053/148‑1 (MQ=255)   gAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCa                                                                                                                                             <  4:285545/148‑1 (MQ=255)     gTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACcttcttatt                                                                                                                                               >  4:269284/1‑144 (MQ=255)     gTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACcttcttatt                                                                                                                                               <  3:269284/144‑1 (MQ=255)        gggCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTg                                                                                                                                       >  7:300754/1‑149 (MQ=255)         ggCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTg                                                                                                                                       <  5:61364/148‑1 (MQ=255)          gCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGc                                                                                                                                     <  3:164876/149‑1 (MQ=255)            aaaaaaTGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCgg                                                                                                                                   <  7:298283/149‑1 (MQ=255)                 aTGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATg                                                                                                                              <  7:267199/149‑1 (MQ=255)                           cGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCaaaa                                                                                                                    >  1:3084/1‑149 (MQ=255)                               tgCGAACTGAGCATAATAATGCCTTTAATCATTTTCTTCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTc                                                                                                                <  1:114793/148‑1 (MQ=255)                                gCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCa                                                                                                               <  3:304592/149‑1 (MQ=255)                                gCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCa                                                                                                               <  2:153870/149‑1 (MQ=255)                                gCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCa                                                                                                               <  6:205956/149‑1 (MQ=255)                                 cGAACTGAGCATAATAATTCCTTTTATCATTTTCTGATTAATTTTGCTTTCGCATTTTTGCAGATCTTCTTCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCTGTGATGGCATGCAAAACTTCag                                                                                                              <  7:118240/149‑1 (MQ=255)                                  gAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCaga                                                                                                             <  3:298911/149‑1 (MQ=255)                                   aaCTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCa                                                                                                               >  2:166961/1‑146 (MQ=255)                                    aCTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAgaga                                                                                                           <  2:3084/149‑1 (MQ=255)                                     cTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGc                                                                                                                            <  7:23239/131‑1 (MQ=255)                                     cTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACATTCTTATTCAGCTTTGGCGGTGATGGc                                                                                                                            >  8:23239/1‑131 (MQ=255)                                      tGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCAGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATTCAAAACTTAAGAGaa                                                                                                          >  3:107534/1‑148 (MQ=255)                                        aGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGaaaaa                                                                                                       >  8:139828/1‑149 (MQ=255)                                         gCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTGATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAAc                                                                                                      >  8:371386/1‑149 (MQ=255)                                         gCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAAc                                                                                                      <  6:258720/149‑1 (MQ=255)                                            taataaTGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGa                                                                                                   <  3:165090/149‑1 (MQ=255)                                            taataaTGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGa                                                                                                   <  3:299835/149‑1 (MQ=255)                                            taataaTGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGa                                                                                                   >  3:315237/1‑149 (MQ=255)                                            taataaTGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGa                                                                                                   >  5:324354/1‑149 (MQ=255)                                               taatGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTa                                                                                                >  1:253150/1‑149 (MQ=255)                                                 atGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTAcc                                                                                              <  5:295008/149‑1 (MQ=255)                                                     cTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACccgcc                                                                                          <  5:271366/149‑1 (MQ=255)                                                           tCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGtt                                                                                    >  1:241680/1‑149 (MQ=255)                                                             aTTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGtttt                                                                                  >  1:59092/1‑149 (MQ=255)                                                              ttttCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTg                                                                                 <  6:60546/149‑1 (MQ=255)                                                               tttCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCaaaa                                                                                                                    >  6:203245/1‑113 (MQ=255)                                                               tttCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCaaaa                                                                                                                    <  5:203245/113‑1 (MQ=255)                                                                 tCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGt                                                                               <  8:202138/148‑1 (MQ=255)                                                                      ttAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACATTCTTATTCAGCTTTGGCGGTGATGGCATGAAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACg                                                                         >  5:290986/1‑149 (MQ=255)                                                                          ttttTCATTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTa                                                                     <  1:24484/149‑1 (MQ=255)                                                                          ttttGCTTTCGCATTTTTGAAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGAGTACCCGCCGTAGTTTTGGTCGAACggg                                                                       >  2:21808/1‑147 (MQ=255)                                                                           tttGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGTTGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGGTTTGGTCGAACggg                                                                       >  4:206623/1‑146 (MQ=255)                                                                               cTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGaaa                                                                 <  4:152155/148‑1 (MQ=255)                                                                                  tCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAc                                                             >  4:356722/1‑149 (MQ=255)                                                                                         ttttCAGATCATCTGCCGGATTGTGCGACGTTAGTAATATCACGTCGAAACATTCTTATTAAGCTTTGGAGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAAt                                                       <  2:323025/148‑1 (MQ=255)                                                                                         tttGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAAt                                                       <  2:215572/148‑1 (MQ=255)                                                                                         tttGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAAt                                                       <  6:166201/148‑1 (MQ=255)                                                                                              aGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCAt                                                  >  7:193726/1‑148 (MQ=255)                                                                                                aTCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCa                                                >  7:233174/1‑148 (MQ=255)                                                                                                       gCCGGATTGTGCGACGTTTGTAATATCACGTCGCCACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGCAAAACGGATTACCCGCCGTAGTTTTGGTCTAACGGGTAGAAATCACCATAATGGCATCAAAAcacc                                         >  5:103936/1‑145 (MQ=255)                                                                                                        ccGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCg                                         >  3:322689/1‑147 (MQ=255)                                                                                                           gATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAagag                                    >  4:283017/1‑149 (MQ=255)                                                                                                            aTTGTGCGACGTTTGTTATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAaga                                     <  7:371386/147‑1 (MQ=255)                                                                                                                   gACGTTTGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAgcgc                            <  3:356722/149‑1 (MQ=255)                                                                                                                    aCGTTTGTAATATCACGTCGAAACCTACTTATTAAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTAACAGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCAACAAAAACCGAAGAGGTCAgagcc                           >  4:298586/1‑148 (MQ=255)                                                                                                                       tttGTAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCgg                        <  8:300754/149‑1 (MQ=255)                                                                                                                       tttGTAATATCAAGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATTGCATGCAAAACTTCAGAGAATAACGGATTACCCGCCGTAGTTTTGGTCGAACTGGTAGAAATCACCATCATGGCATCAAAACCCGAAGATGTCAGCGCGCgg                        <  6:103936/149‑1 (MQ=255)                                                                                                                           tAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAccc                                          <  8:296003/127‑1 (MQ=255)                                                                                                                           tAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAccc                                          >  7:296003/1‑127 (MQ=255)                                                                                                                           tAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCa                    <  3:232789/149‑1 (MQ=255)                                                                                                                           tAATATCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCa                    <  8:193726/149‑1 (MQ=255)                                                                                                                              tatCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCt                 <  4:315237/149‑1 (MQ=255)                                                                                                                                tCACGTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTg               <  2:253150/149‑1 (MQ=255)                                                                                                                                    gTCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTg           <  3:149374/149‑1 (MQ=255)                                                                                                                                     tCGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTgg          <  2:59092/149‑1 (MQ=255)                                                                                                                                      cGAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCCTCAAACCCCGAAGAGGTCAGCGCGCGGGCCCGCTAGTTTGGc         >  6:45910/1‑149 (MQ=255)                                                                                                                                       gAAACCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGc         <  6:324354/148‑1 (MQ=255)                                                                                                                                          aCCTTCTTATTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGAAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGa      >  3:282376/1‑148 (MQ=255)                                                                                                                                            cttcttattCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAc   >  7:159717/1‑149 (MQ=255)                                                                                                                                             ttcttattcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACt  >  7:313388/1‑149 (MQ=255)                                                                                                                                             ttcttattcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAAAGGATTACCCGCCGTAGTTTTGTTCGAACGTGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTTATACt  >  1:90652/1‑149 (MQ=255)                                                                                                                                               cttattcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACt  <  3:298586/147‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                TCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACT  >  NC_000913/1383540‑1383828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑