| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,983,820 | T→C | 6.0% | D102G (GAC→GGC) | kduI ← | hexuronate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,983,820 | 0 | T | C | 6.0% | 208.0 / 3.0 | 66 | D102G (GAC→GGC) | kduI | hexuronate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (32/30); new base C (2/2); total (34/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
GACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTA > NC_000913/2983675‑2983963 | gACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTcg > 7:106259/1‑148 (MQ=255) aCTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTcgc < 3:242512/148‑1 (MQ=255) agaTACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGa > 2:134228/1‑148 (MQ=255) gaTACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGAc > 1:184271/1‑148 (MQ=255) tACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGAcc > 5:117888/1‑147 (MQ=255) gTCCGGTGTGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCa < 7:315582/148‑1 (MQ=255) gtgtGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCa < 8:106259/148‑1 (MQ=255) tgACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTCCCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTg < 6:204963/148‑1 (MQ=255) tgACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTg < 2:184271/148‑1 (MQ=255) gACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGc < 1:113937/143‑1 (MQ=255) gACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGc > 2:113937/1‑143 (MQ=255) gACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTgg > 3:129521/1‑148 (MQ=255) aCTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTgg < 6:117888/147‑1 (MQ=255) cTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTgg > 6:208127/1‑146 (MQ=255) cTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTgg < 5:208127/146‑1 (MQ=255) tttttGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCAGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCAt > 7:92849/1‑148 (MQ=255) tttttGGTGGGATACGTCGTATGCGCGGGTGCACAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTAATAGCATTGGCCAt > 1:78848/1‑148 (MQ=255) gggATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCa < 4:57975/132‑1 (MQ=255) gggATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCa > 3:57975/1‑132 (MQ=255) aTACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATc > 4:208998/1‑148 (MQ=255) aCGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCg > 8:306049/1‑147 (MQ=255) tcgtATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTAcc > 1:212670/1‑148 (MQ=255) gTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAat < 6:218655/148‑1 (MQ=255) cgcAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAatat < 7:306049/147‑1 (MQ=255) aTTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGAt > 6:114184/1‑147 (MQ=255) aTTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATa < 7:293555/148‑1 (MQ=255) tGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTaa > 3:169782/1‑120 (MQ=255) tGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTaa < 4:169782/120‑1 (MQ=255) gTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATa > 2:294573/1‑145 (MQ=255) gTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATa < 1:294573/145‑1 (MQ=255) aataaAACTTCGCCGGAGTGCCGGTATCATTACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACAcc > 2:199185/1‑148 (MQ=255) taaAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCt > 1:75345/1‑147 (MQ=255) aaCTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGAc < 3:301155/107‑1 (MQ=255) aaCTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGAc > 4:301155/1‑107 (MQ=255) aCTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTGTGCACCTTTACCGCCATACAGGGCGTCGCGGGGCCCGATGTCATAGCATTGGCCCTCGACAGTCATCGTCCAGGCCCCGCCAATATTGATAACCCCTAACTc > 6:214876/1‑148 (MQ=255) cGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTcgc < 1:134228/146‑1 (MQ=255) gCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACa > 5:277786/1‑94 (MQ=255) gCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACa < 6:277786/94‑1 (MQ=255) cGGTATCATTACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGaa < 1:199185/148‑1 (MQ=255) cGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGaa < 3:208998/148‑1 (MQ=255) aaTACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATGGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCtt < 5:261861/148‑1 (MQ=255) aCTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATa > 1:206384/1‑112 (MQ=255) aCTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATa < 2:206384/112‑1 (MQ=255) cTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGc < 4:89130/148‑1 (MQ=255) gCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAg < 1:281396/138‑1 (MQ=255) cAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGAATTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCCCCGATCAAGGAAATAGc > 2:281396/1‑138 (MQ=255) ttcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCa > 1:296154/1‑118 (MQ=255) ttcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCa < 2:296154/118‑1 (MQ=255) tcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCaa > 3:257935/1‑148 (MQ=255) tttGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGa < 6:195827/108‑1 (MQ=255) tttGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGa > 5:195827/1‑108 (MQ=255) ttGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTc > 8:88818/1‑148 (MQ=255) tttACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCaa > 2:216366/1‑107 (MQ=255) tttACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCaa < 1:216366/107‑1 (MQ=255) ttACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCaa > 7:254117/1‑148 (MQ=255) aaCATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGa > 6:100447/1‑147 (MQ=255) aCATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGaaa > 7:275392/1‑148 (MQ=255) aCAGGGCGCCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACAGCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAgttgt < 1:199940/119‑1 (MQ=255) aCAGGGCGCCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAgttgt > 2:199940/1‑119 (MQ=255) aCAGGGCGCCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAgttgt < 1:195367/119‑1 (MQ=255) aCAGGGCGCCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAgttgt > 2:195367/1‑119 (MQ=255) cAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGtt < 5:100447/148‑1 (MQ=255) aGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAATGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGttt < 8:92849/148‑1 (MQ=255) ggCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAAGCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTa < 7:294829/148‑1 (MQ=255) gCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGtt < 4:189466/144‑1 (MQ=255) gCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGtt > 3:189466/1‑144 (MQ=255) | GACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTA > NC_000913/2983675‑2983963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |