Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,034,140 C→A 7.7% L345L (CTG→CTT lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,034,1400CA 7.7% 157.5 / 3.4 52L345L (CTG→CTTlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (25/23);  new base A (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

CGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGC  >  NC_000913/3033993‑3034287
                                                                                                                                                   |                                                                                                                                                   
cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTcc                                                                                                                                                     <  7:245928/148‑1 (MQ=255)
         aGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGcc                                                                                                                                            <  8:26271/148‑1 (MQ=255)
         aGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGcc                                                                                                                                            <  1:35365/148‑1 (MQ=255)
                    tCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTc                                                                                                                                 >  4:144947/1‑148 (MQ=255)
                        cTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGGTTCCggg                                                                                                                             >  6:17814/1‑148 (MQ=255)
                           aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGa                                                                                                                          >  2:142417/1‑148 (MQ=255)
                                      cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGAt                                                                                                               <  3:262574/148‑1 (MQ=255)
                                      cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGAt                                                                                                               <  7:330297/148‑1 (MQ=255)
                                              aCTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCAc                                                                                                       <  1:142417/148‑1 (MQ=255)
                                                 tcttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACg                                                                                                      >  6:15378/1‑146 (MQ=255)
                                                  cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGc                                                                                                     <  5:15378/146‑1 (MQ=255)
                                                       aaGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACTTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCAAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGt                                                                                                >  2:295828/1‑146 (MQ=255)
                                                       aaGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCAAGATCCGCCATGTCGGGTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGt                                                                                                >  2:291377/1‑146 (MQ=255)
                                                        aGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAg                                                                                             >  4:10087/1‑148 (MQ=255)
                                                         gATCTGGGTTACGATACGGCCCAGACCCCAGCGCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGTAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGAGCGCTTCACGCTTTGTCAg                                                                                             <  4:203428/147‑1 (MQ=255)
                                                           tctcGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGttt                                                                                          >  1:279565/1‑148 (MQ=255)
                                                               ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGCCAGttttt                                                                                        <  8:951/146‑1 (MQ=255)
                                                                    cGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACgg                                                                                 <  3:144947/148‑1 (MQ=255)
                                                                                    ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGatat                                                                                                                       >  3:295398/1‑94 (MQ=255)
                                                                                    ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGatat                                                                                                                       <  4:295398/94‑1 (MQ=255)
                                                                                    ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGc                                                                                                            >  6:236417/1‑105 (MQ=255)
                                                                                    ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGc                                                                                                            <  5:236417/105‑1 (MQ=255)
                                                                                    ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACGGAAGTCCAGc                                                                 >  8:244023/1‑148 (MQ=255)
                                                                                    ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGc                                                                 >  6:188171/1‑148 (MQ=255)
                                                                                      aGCTCTTCTCAACGTGGATGCCGATAGATTCAGAAATTGCTTTCGCAGAGGCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGt                                                               <  7:69595/148‑1 (MQ=255)
                                                                                       gCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGt                                                               >  7:254761/1‑147 (MQ=255)
                                                                                        ctctTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCa                                                             <  8:60210/148‑1 (MQ=255)
                                                                                            tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTc                                                         >  1:319202/1‑148 (MQ=255)
                                                                                             ctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCg                                                        >  6:138491/1‑148 (MQ=255)
                                                                                                            gATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTcc                                         >  8:147696/1‑148 (MQ=255)
                                                                                                             aTAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCg                                        >  4:100778/1‑148 (MQ=255)
                                                                                                             aTAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGGCACGGCGCCGTAGGTCACTTCCg                                        <  5:17814/148‑1 (MQ=255)
                                                                                                                gATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCt                                     <  5:188171/148‑1 (MQ=255)
                                                                                                                     aGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCg                                <  6:139376/148‑1 (MQ=255)
                                                                                                                       cAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCAGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTAcc                                 >  7:318756/1‑145 (MQ=255)
                                                                                                                         aTTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCg                                <  3:176790/144‑1 (MQ=255)
                                                                                                                         aTTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCg                                >  4:176790/1‑144 (MQ=255)
                                                                                                                           tGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAgg                                                 <  8:323051/125‑1 (MQ=255)
                                                                                                                           tGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAgg                                                 >  7:323051/1‑125 (MQ=255)
                                                                                                                             cTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATc                        <  5:138491/148‑1 (MQ=255)
                                                                                                                                  gCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCtgtg                   <  8:254761/148‑1 (MQ=255)
                                                                                                                                   cAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGc                  >  7:208775/1‑148 (MQ=255)
                                                                                                                                    agagTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCAGCTGGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGc                  >  1:85587/1‑147 (MQ=255)
                                                                                                                                      agTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCag               <  7:147696/148‑1 (MQ=255)
                                                                                                                                         cGAAGTTGTCCAGATCAGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCagcg             >  1:301074/1‑145 (MQ=255)
                                                                                                                                           aaGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGCTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTAc          >  5:266868/1‑148 (MQ=255)
                                                                                                                                             gTTGTCAAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGAGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACgg        <  1:295828/148‑1 (MQ=255)
                                                                                                                                             gTTGTCAAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGAGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACgg        <  1:291377/148‑1 (MQ=255)
                                                                                                                                              ttGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACgg        >  4:213302/1‑147 (MQ=255)
                                                                                                                                              ttGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGa       <  3:175118/148‑1 (MQ=255)
                                                                                                                                                gTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGaa      >  2:273083/1‑147 (MQ=255)
                                                                                                                                                 tCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGaa      >  4:226718/1‑146 (MQ=255)
                                                                                                                                                 tCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAAc     <  8:208775/147‑1 (MQ=255)
                                                                                                                                                  ccAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAg   >  7:190933/1‑148 (MQ=255)
                                                                                                                                                   cAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGc  >  7:139228/1‑148 (MQ=255)
                                                                                                                                                   cAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGc  >  4:63177/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGC  >  NC_000913/3033993‑3034287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: