Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,341,395 A→T 6.1% K44* (AAG→TAG)  kdsD → D‑arabinose 5‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,341,3950AT 6.1% 257.8 / 4.9 80K44* (AAG→TAG) kdsDD‑arabinose 5‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (38/37);  new base T (3/2);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCA  >  NC_000913/3341254‑3341542
                                                                                                                                             |                                                                                                                                                   
tGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGt                                                                                                                                               >  6:109458/1‑148 (MQ=255)
tGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGt                                                                                                                                               >  2:302835/1‑148 (MQ=255)
  gAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGtt                                                                                                                                              <  8:162649/147‑1 (MQ=255)
  gAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGt                                                                                                                                               <  6:68797/146‑1 (MQ=255)
   aaaCGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCt                                                                                                                                            <  1:41007/148‑1 (MQ=255)
     aCGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTgg                                                                                                                                          <  2:151823/148‑1 (MQ=255)
        cATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGgtgt                                                                                                                                       <  3:300060/148‑1 (MQ=255)
             tGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCACTACATCAATCAGAATTTCACGATTGCCTGTGAAAAGATGTTCTGGTGTaaa                                                                                                                                    <  4:135556/146‑1 (MQ=255)
              gTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAgg                                                                                                                                  >  5:201947/1‑147 (MQ=255)
                cGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGa                                                                                                                                >  3:254910/1‑147 (MQ=255)
                      tAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGACTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTTGTGTAAAGTGAAAGTTgt                                                                                                                         >  5:71048/1‑148 (MQ=255)
                          gTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgtcgtc                                                                                                                     <  3:160391/148‑1 (MQ=255)
                            tACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGaa                                                                                                                               <  1:57979/136‑1 (MQ=255)
                            tACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGaa                                                                                                                               >  2:57979/1‑136 (MQ=255)
                            tACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCAt                                                                                                                   >  4:57014/1‑148 (MQ=255)
                            tACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCAt                                                                                                                   >  7:170625/1‑148 (MQ=255)
                                aCCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATgggg                                                                                                               <  6:296440/148‑1 (MQ=255)
                                aCCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATggg                                                                                                                >  5:185538/1‑147 (MQ=255)
                                  cGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGAt                                                                                                             >  7:220751/1‑148 (MQ=255)
                                   gggTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATg                                                                                                            <  6:306444/148‑1 (MQ=255)
                                            cTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCg                                                                                                   <  4:103783/148‑1 (MQ=255)
                                             tttCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCgg                                                                                                  <  6:201947/148‑1 (MQ=255)
                                               tCAGCAAGCAGGTAAAGAAGTCATGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCggg                                                                                                 <  5:109458/147‑1 (MQ=255)
                                                   caagcaGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCata                                                                                            <  1:200583/148‑1 (MQ=255)
                                                     agcaGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCat                                                                                             <  7:160756/145‑1 (MQ=255)
                                                     agcaGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCat                                                                                             >  8:160756/1‑145 (MQ=255)
                                                      gcaGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTg                                                                                         <  3:57014/148‑1 (MQ=255)
                                                         ggTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAATAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTgggg                                                                                      >  8:111268/1‑147 (MQ=255)
                                                          gTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTgg                                                                                        <  5:82712/145‑1 (MQ=255)
                                                          gTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTgg                                                                                        >  6:82712/1‑145 (MQ=255)
                                                           tAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCg                                                                                     >  6:185536/1‑147 (MQ=255)
                                                            aaaGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGa                                                                                    >  5:19070/1‑147 (MQ=255)
                                                               gaagTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgtcgtc                                                                                                                     >  1:205698/1‑111 (MQ=255)
                                                               gaagTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgtcgtc                                                                                                                     <  2:205698/111‑1 (MQ=255)
                                                               gaagTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGaaaaa                                                                                >  5:47275/1‑148 (MQ=255)
                                                                aagTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAAt                                                                               <  1:238607/148‑1 (MQ=255)
                                                                    ccTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATggcg                                                                           >  5:229370/1‑148 (MQ=255)
                                                                      tGGCGATTGAACGTGTATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGaaaaa                                                                                <  6:259609/141‑1 (MQ=255)
                                                                      tGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgt                                                                                                                         >  1:241182/1‑100 (MQ=255)
                                                                      tGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgt                                                                                                                         <  2:241182/100‑1 (MQ=255)
                                                                      tGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGaaaaa                                                                                >  5:259609/1‑141 (MQ=255)
                                                                       ggCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGaaaa                                                                                 <  4:11123/139‑1 (MQ=255)
                                                                       ggCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGaaaa                                                                                 >  3:11123/1‑139 (MQ=255)
                                                                       ggCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATggcggc                                                                         >  6:21120/1‑147 (MQ=255)
                                                                        gCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAATAGATGTTCTGGTGTAAAGGGAAAGTTgtcgtc                                                                                                                     >  8:131128/1‑102 (MQ=255)
                                                                        gCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAATAGATGTTCTGGTGTAAAGGGAAAGTTgtcgtc                                                                                                                     <  7:131128/102‑1 (MQ=255)
                                                                         cGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAAc                                                                      <  6:47275/148‑1 (MQ=255)
                                                                              gAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGttt                                                                  <  7:78531/147‑1 (MQ=255)
                                                                                aCGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGcc                                                               <  5:246053/148‑1 (MQ=255)
                                                                                      aTGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCAcc                                                         <  4:18133/148‑1 (MQ=255)
                                                                                         ccTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAATAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGTGAAAAATGGCGGCAACGTTTGCCAGCACCGGt                                                      <  7:111268/148‑1 (MQ=255)
                                                                                              cGGAGATTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACAcc                                                 <  7:325941/148‑1 (MQ=255)
                                                                                                      gATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCAtttt                                         >  5:110136/1‑148 (MQ=255)
                                                                                                      gATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCAtttt                                         >  3:94897/1‑148 (MQ=255)
                                                                                                          aaTACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGaa                                                                                   >  1:35433/1‑102 (MQ=255)
                                                                                                          aaTACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGaa                                                                                   <  2:35433/102‑1 (MQ=255)
                                                                                                          aaTACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCg                                      <  4:254910/147‑1 (MQ=255)
                                                                                                              cATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCAt                                 <  8:220751/148‑1 (MQ=255)
                                                                                                               atcaatcaGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATc                                >  6:245801/1‑148 (MQ=255)
                                                                                                                tcaatcaGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATcc                               >  3:104151/1‑148 (MQ=255)
                                                                                                                 caatcaGAATTTCACGCTTGCCTGTGAATAGATGTTCTGGTGTAAAGGGAAAGTTGTTGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCt                              >  1:136584/1‑148 (MQ=255)
                                                                                                                 caatcaGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCg                                                                                                   >  4:83424/1‑79 (MQ=255)
                                                                                                                 caatcaGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCg                                                                                                   <  3:83424/79‑1 (MQ=255)
                                                                                                                 caatcaGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCt                              >  8:76503/1‑148 (MQ=255)
                                                                                                                  aatcgGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTg                             >  4:214451/1‑148 (MQ=255)
                                                                                                                   atcaGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAATATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTcc                                   <  3:3698/141‑1 (MQ=255)
                                                                                                                    tcaGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGt                           >  2:308563/1‑148 (MQ=255)
                                                                                                                    tcaGAATTTCACCCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGCGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGTCGGCTACGTTTGCCAGCACCGGTACCCCTTCATTTATCGTCCAtagatcg                           >  4:3698/1‑142 (MQ=255)
                                                                                                                      aGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTg                          <  5:245801/147‑1 (MQ=255)
                                                                                                                       gAATTTCACGCTTGCCTTTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGTTACACCTTCATTTTTCGTCCATCCTGGTGaa                        <  6:71048/148‑1 (MQ=255)
                                                                                                                         aTTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGc                      >  5:70622/1‑148 (MQ=255)
                                                                                                                          tttCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGcc                     >  7:185849/1‑148 (MQ=255)
                                                                                                                           ttCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTgg                            <  7:314408/140‑1 (MQ=255)
                                                                                                                           ttCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTgg                            >  8:314408/1‑140 (MQ=255)
                                                                                                                           ttCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCcg                    >  8:34897/1‑148 (MQ=255)
                                                                                                                               cGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCa                <  3:214451/148‑1 (MQ=255)
                                                                                                                                       tgtgAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGAtt        >  3:152813/1‑148 (MQ=255)
                                                                                                                                       tgtgAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGAtt        >  4:264750/1‑148 (MQ=255)
                                                                                                                                          gAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGt                                                      <  1:53157/99‑1 (MQ=255)
                                                                                                                                          gAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGt                                                      >  2:53157/1‑99 (MQ=255)
                                                                                                                                             aaGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCa  <  6:229370/148‑1 (MQ=255)
                                                                                                                                             aaGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGAAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCa  <  8:170625/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
TGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCA  >  NC_000913/3341254‑3341542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: