Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,390,707	A→G	5.1%	V441A (GTT→GCT)	tldD ←	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,390,707	0	A	G	5.1%	248.1 / 2.5	78	V441A (GTT→GCT)	tldD	putative peptidase
Reads supporting (aligned to +/- strand):  ref base A (40/34);  new base G (2/2);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGC  >  NC_000913/3390564‑3390851                                                                                                                                                |                                                                                                                                                 tCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGGCGTTATCCAGTTTCAGGTCGTTGCCAACCAt                                                                                                                                              >  1:312558/1‑148 (MQ=255) tCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCAt                                                                                                                                              >  5:177499/1‑148 (MQ=255)  cTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCAt                                                                                                                                              <  4:251241/147‑1 (MQ=255)      aaaTATTTAAATTATTACGCAGTACCGCCAACAGTCAGTTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGaa                                                                                                                                          <  6:142674/147‑1 (MQ=255)             gAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACGATCGAAATctgct                                                                                                                                   >  2:65811/1‑147 (MQ=255)              aaaTTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATctgctg                                                                                                                                  >  3:167466/1‑147 (MQ=255)              aaaTTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATctgctg                                                                                                                                  >  8:151230/1‑147 (MQ=255)              aaaTTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCtgctgc                                                                                                                                 <  6:293723/148‑1 (MQ=255)                  tattaCGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATgg                                                                                                                             >  7:226840/1‑148 (MQ=255)                           gTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTCCCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATAc                                                                                                                    <  6:256903/148‑1 (MQ=255)                            tACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAGCCATCGAAATCTGCTGCATGGTTTCGATAcc                                                                                                                   >  8:286107/1‑148 (MQ=255)                             aCCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCg                                                                                                                  >  1:284122/1‑148 (MQ=255)                             aCCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCg                                                                                                                  >  5:279112/1‑148 (MQ=255)                                   aaCAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAGCCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCa                                                                                                            <  7:286107/148‑1 (MQ=255)                                   aaCAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCa                                                                                                            <  4:167466/148‑1 (MQ=255)                                        tCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTATCGATACCGGAACCAATCaa                                                                                                       <  2:253654/148‑1 (MQ=255)                                         cAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGa                                                                                                                                           >  7:143224/1‑111 (MQ=255)                                         cAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGa                                                                                                                                           <  8:143224/111‑1 (MQ=255)                                         cAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAAc                                                                                                      <  6:279112/148‑1 (MQ=255)                                            gTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGtt                                                                                                   >  7:225/1‑148 (MQ=255)                                            gTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGtt                                                                                                   >  2:24681/1‑148 (MQ=255)                                             ttATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTAGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTg                                                                                                  >  8:307370/1‑148 (MQ=255)                                               aTCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAGCCATCGaaa                                                                                                                                         <  5:124998/107‑1 (MQ=255)                                               aTCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAGCCATCGaaa                                                                                                                                         >  6:124998/1‑107 (MQ=255)                                                tCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGCCACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGCACCAATCAACGTTgcgc                                                                                               >  8:243407/1‑148 (MQ=255)                                                   aCTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCtt                                                                                            >  7:282188/1‑148 (MQ=255)                                                      ttCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCa                                                                                         >  7:193987/1‑148 (MQ=255)                                                      ttCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCa                                                                                         >  1:199413/1‑148 (MQ=255)                                                       tCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACg                                                                                                     >  6:51250/1‑135 (MQ=255)                                                       tCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACg                                                                                                     <  5:51250/135‑1 (MQ=255)                                                       tCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTc                                                                                          <  2:284122/146‑1 (MQ=255)                                                       tCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTc                                                                                          <  2:199413/146‑1 (MQ=255)                                                       tCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCAc                                                                                        >  6:101705/1‑148 (MQ=255)                                                        cAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGttt                                                                                                                          <  6:33254/113‑1 (MQ=255)                                                        cAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGttt                                                                                                                          >  5:33254/1‑113 (MQ=255)                                                          aCGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCgg                                                                                     >  8:119478/1‑148 (MQ=255)                                                          aCGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCgg                                                                                     <  8:226840/148‑1 (MQ=255)                                                            gTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGGTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCt                                                                                   <  6:177499/148‑1 (MQ=255)                                                                       cACGCCCACCGGCAAACTTTGCCCTTCTTTACCGCAGCCCCCCCCGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATCCCGGAACCAATCAACGTTGCGCCTTTCCCCGGCTTCGTTGCtttt                                                                        >  4:113906/1‑147 (MQ=255)                                                                           ccAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCa                                                                                                        >  8:133422/1‑112 (MQ=255)                                                                           ccAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCa                                                                                                        <  7:133422/112‑1 (MQ=255)                                                                            cAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGtt                                                                   <  8:282188/148‑1 (MQ=255)                                                                            cAACCGGCAAACTTTGCCCTTCTTTACCACAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGtt                                                                   <  7:288371/148‑1 (MQ=255)                                                                             aaCCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTc                                                                                          <  5:52312/124‑1 (MQ=255)                                                                             aaCCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTc                                                                                          >  6:52312/1‑124 (MQ=255)                                                                             aaCCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGttt                                                                  >  7:302841/1‑148 (MQ=255)                                                                             aaCCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGttt                                                                  >  4:62426/1‑148 (MQ=255)                                                                              aCCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTAc                                                                            >  1:173952/1‑137 (MQ=255)                                                                              aCCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTAc                                                                            <  2:173952/137‑1 (MQ=255)                                                                               ccGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTc                                                                                          >  6:194999/1‑122 (MQ=255)                                                                               ccGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTc                                                                                          <  5:194999/122‑1 (MQ=255)                                                                                cGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCa                                                               <  7:307370/148‑1 (MQ=255)                                                                                     aaCTTTGCCCTTCTTTACCGCAGACACCGACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAg                                                          <  5:101705/148‑1 (MQ=255)                                                                                     aaCTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAAt                                                             >  4:265954/1‑145 (MQ=255)                                                                                     aaCTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAAt                                                             >  2:268131/1‑145 (MQ=255)                                                                                     aaCTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAg                                                          <  1:24681/148‑1 (MQ=255)                                                                                                tcttTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCt                                                >  3:6283/1‑147 (MQ=255)                                                                                                   ttACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGaa                                             >  7:120936/1‑147 (MQ=255)                                                                                                   ttACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGaa                                             <  8:120936/147‑1 (MQ=255)                                                                                                      ccgccgACACCCACGCCGTTATGCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTg                                          <  6:223184/147‑1 (MQ=255)                                                                                                      ccGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTgg                                         <  4:6283/148‑1 (MQ=255)                                                                                                             cacCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGt                                                                               <  5:208380/103‑1 (MQ=255)                                                                                                             cacCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGt                                                                               >  6:208380/1‑103 (MQ=255)                                                                                                              acCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACg                                 <  3:265954/148‑1 (MQ=255)                                                                                                               cccACGCCGTTATCCAGTTTCAGGTCTTTGACAACCATCGAAATCTGCAGCATGGTTTCGATACCGGAACCAATCAACGTAGAGCCGATCACCGGCTTCGTTACTTTACCGTTTTCAATCTGATATGCTGCTGAAGTGGAGAAAACGa                                >  2:234435/1‑148 (MQ=255)                                                                                                               cccACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACg                                 >  4:192926/1‑147 (MQ=255)                                                                                                               cccACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGa                                <  4:80931/148‑1 (MQ=255)                                                                                                                   cGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTGCACCGGCTTCGTTACTTTACAGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAAttt                            >  1:184419/1‑148 (MQ=255)                                                                                                                    gCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGa                                              >  7:43043/1‑129 (MQ=255)                                                                                                                    gCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGa                                              <  8:43043/129‑1 (MQ=255)                                                                                                                      cGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGcc                         <  8:302841/148‑1 (MQ=255)                                                                                                                            ccAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACttt                                                                         >  8:18926/1‑94 (MQ=255)                                                                                                                            ccAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACttt                                                                         <  7:18926/94‑1 (MQ=255)                                                                                                                               gTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGat                <  7:119478/148‑1 (MQ=255)                                                                                                                                  tCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGtt                                                                              >  4:155073/1‑83 (MQ=255)                                                                                                                                  tCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGtt                                                                              <  3:155073/83‑1 (MQ=255)                                                                                                                                      gTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCAcc         >  8:40475/1‑148 (MQ=255)                                                                                                                                              cAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGc  >  3:34000/1‑147 (MQ=255)                                                                                                                                                |                                                                                                                                                 TCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGC  >  NC_000913/3390564‑3390851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑