Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,354,100 T→A 6.1% V206V (GTA→GTT)  lysU ← lysine tRNA synthetase, inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,354,1000TA 6.1% 209.1 / 3.7 65V206V (GTA→GTT) lysUlysine tRNA synthetase, inducible
Reads supporting (aligned to +/- strand):  ref base T (34/27);  new base A (2/2);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

TACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTG  >  NC_000913/4353953‑4354237
                                                                                                                                                   |                                                                                                                                         
tACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCt                                                                                                                                           <  7:163147/148‑1 (MQ=255)
  cccGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTa                                                                                                                                          <  1:304174/147‑1 (MQ=255)
      ttCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCACCTTc                                                                                                                                      >  3:276874/1‑147 (MQ=255)
      ttCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATATAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCAACTTc                                                                                                                                      >  3:281331/1‑147 (MQ=255)
      ttCAAAACCGCCTACAACCAGACGGTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCAGGAATTACCTGCATCATCGGGGTTTCTACTTc                                                                                                                                      >  7:101253/1‑147 (MQ=255)
           aaCCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATa                                                                                                                                  >  4:302135/1‑146 (MQ=255)
                 cTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAACGCcgcg                                                                                                                          >  5:48226/1‑148 (MQ=255)
                       ccAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGAc                                                                                                                    >  2:244294/1‑148 (MQ=255)
                        cAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGAc                                                                                                                    >  3:153455/1‑147 (MQ=255)
                          gACCTTTCAGATACAGCTCCGGCGCGATACGCAGATACAAATCTAAATCCAGAGAATTATGATGGGTAATAAACTGGAGAGAAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTACTACTTCCATAAAGCCGCGCGCGACCAt                                                                                                                 >  2:167214/1‑148 (MQ=255)
                            cGTTTCAGATACAGCTACGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGa                                                                                                               <  5:21632/148‑1 (MQ=255)
                             gTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGaa                                                                                                              <  1:134001/148‑1 (MQ=255)
                               ttCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAAtt                                                                                                            >  3:47752/1‑148 (MQ=255)
                                tCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAAtt                                                                                                            <  8:101253/147‑1 (MQ=255)
                                   gATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGAc                                                                                                         >  6:243042/1‑147 (MQ=255)
                                   gATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGAc                                                                                                         <  5:243042/147‑1 (MQ=255)
                                    aTACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACg                                                                                                        >  5:182448/1‑147 (MQ=255)
                                    aTACAGCTCCGGCGCGATACCCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACg                                                                                                        >  6:302034/1‑147 (MQ=255)
                                            ccGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGc                                                                                               >  1:259091/1‑148 (MQ=255)
                                             cGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGcc                                                                                              <  4:153455/148‑1 (MQ=255)
                                                    aTACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAAttt                                                                                       <  1:244294/148‑1 (MQ=255)
                                                     tACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAAtttt                                                                                      <  8:6692/148‑1 (MQ=255)
                                                        gCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGa                                                                                   <  6:277051/148‑1 (MQ=255)
                                                          agatagatATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAAc                                                                                 >  8:188288/1‑148 (MQ=255)
                                                            aTACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACg                                                                                >  8:324655/1‑147 (MQ=255)
                                                                 tatCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGacaa                                                                           >  4:200078/1‑147 (MQ=255)
                                                                   tcaaaaTGCAGAGCATTATGATGTGTAATAAACGGGCGAGCAGATGCCCCAACTGGAATTACCTGGATCATAGGGTTTTCTTCTTCCTTAAAGCCGCGCTCGACCATGAAATGACGGATAGCGGCCAGAATTTTTTAACGGAcaacaa                                                                        <  1:167214/145‑1 (MQ=255)
                                                                   tCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGacaaca                                                                         >  7:323157/1‑147 (MQ=255)
                                                                       aaTCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGt                                                                    >  4:91411/1‑148 (MQ=255)
                                                                            agagCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGAc                                                               <  3:302135/148‑1 (MQ=255)
                                                                                   tATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGAtt                                                         >  4:38188/1‑147 (MQ=255)
                                                                                    atgatgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGAttt                                                        <  3:91411/147‑1 (MQ=255)
                                                                                     tgatgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTa                                                       <  2:259091/147‑1 (MQ=255)
                                                                                       atgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATc                                                     <  6:182448/147‑1 (MQ=255)
                                                                                        tgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCg                                                    <  5:302034/147‑1 (MQ=255)
                                                                                        tgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGt                                                   >  8:136736/1‑148 (MQ=255)
                                                                                               taaACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTg                                                                 <  7:185552/127‑1 (MQ=255)
                                                                                               taaACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTg                                                                 >  8:185552/1‑127 (MQ=255)
                                                                                               taaACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGAt                                            <  3:38188/148‑1 (MQ=255)
                                                                                                aaaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCg                                                                                                 >  1:32674/1‑94 (MQ=255)
                                                                                                aaaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCg                                                                                                 <  2:32674/94‑1 (MQ=255)
                                                                                                aaaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCg                                              >  5:253757/1‑145 (MQ=255)
                                                                                                aaaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCg                                              <  6:253757/145‑1 (MQ=255)
                                                                                                 aaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGa                                          <  3:200078/148‑1 (MQ=255)
                                                                                                        gAGCATATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCAACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAg                                   <  4:281331/148‑1 (MQ=255)
                                                                                                        gAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGAGACCATGAATTGACGTATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAg                                   <  5:267743/148‑1 (MQ=255)
                                                                                                        gAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCAACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAg                                   <  4:276874/148‑1 (MQ=255)
                                                                                                          gCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATTAGGTCCAGa                                  >  6:169691/1‑147 (MQ=255)
                                                                                                                   ccACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGAc                        >  3:204098/1‑148 (MQ=255)
                                                                                                                    cACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGa                                  >  7:131722/1‑137 (MQ=255)
                                                                                                                    cACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGa                                  <  8:131722/137‑1 (MQ=255)
                                                                                                                    cACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATTACGTTGAc                        >  2:98036/1‑147 (MQ=255)
                                                                                                                    cACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGAc                        >  2:43632/1‑147 (MQ=255)
                                                                                                                        tGGAATTACCTGCATCATCGGGGTTTCTACTTCCATACAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATaa                   >  3:223549/1‑148 (MQ=255)
                                                                                                                        tGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGAc                                                               <  7:40435/104‑1 (MQ=255)
                                                                                                                        tGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGAc                                                               >  8:40435/1‑104 (MQ=255)
                                                                                                                        tGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATaa                   >  7:87360/1‑148 (MQ=255)
                                                                                                                          gAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATaa                   <  7:324655/146‑1 (MQ=255)
                                                                                                                              tACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACt             >  1:246020/1‑148 (MQ=255)
                                                                                                                                       catcGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACg                       <  5:224473/129‑1 (MQ=255)
                                                                                                                                       catcGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACg                       >  6:224473/1‑129 (MQ=255)
                                                                                                                                       catcGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATcc    >  5:98022/1‑148 (MQ=255)
                                                                                                                                        atcGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCt   <  7:188288/148‑1 (MQ=255)
                                                                                                                                         tcGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTg  >  4:290776/1‑148 (MQ=255)
                                                                                                                                          cGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAgg                                        <  7:20964/109‑1 (MQ=255)
                                                                                                                                          cGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAgg                                        >  8:20964/1‑109 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
TACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTG  >  NC_000913/4353953‑4354237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑