Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 441,772 A→G 6.1% H75R (CAT→CGT)  thiI → tRNA s(4)U8 sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913441,7720AG 6.1% 218.4 / 3.5 69H75R (CAT→CGT) thiItRNA s(4)U8 sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base A (28/36);  new base G (2/2);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

ACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGT  >  NC_000913/441634‑441919
                                                                                                                                          |                                                                                                                                                   
aCATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcga                                                                                                                                            <  5:252238/148‑1 (MQ=255)
  aTTCGTAACGTTTTACAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaag                                                                                                                                          <  6:238404/148‑1 (MQ=255)
        aaCGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcg                                                                                                                                       >  2:16343/1‑145 (MQ=255)
         aCGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcgaag                                                                                                                                    <  7:293924/147‑1 (MQ=255)
         aCGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGa                                                                                                                                   <  8:235258/148‑1 (MQ=255)
            ttttAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACTCTCTGACCCGTATTCCGGGTATCCACCTTATTCTCGAAGTCGAAGACGt                                                                                                                                >  8:326184/1‑148 (MQ=255)
             tttAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcgaag                                                                                                                                    >  7:171790/1‑143 (MQ=255)
             tttAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcgaag                                                                                                                                    <  8:171790/143‑1 (MQ=255)
                 aaGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGt                                                                                                                           <  1:131722/148‑1 (MQ=255)
                     aCTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTAc                                                                                                                       <  2:57353/148‑1 (MQ=255)
                        atgatgAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGttt                                                                                                                         <  7:224622/143‑1 (MQ=255)
                        atgatgAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGttt                                                                                                                         >  8:224622/1‑143 (MQ=255)
                         tgatgaGACGCTCGCTGTCGTCCGCCACTGTGATAACATCGAAGTTCGCGAAAAAGATGAAAACCAGCGTCTTGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCAACATATTCTCGAAGTCGAAGACGTGCCGTTTACCGa                                                                                                                    <  5:102222/147‑1 (MQ=255)
                         tgatgaGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGAc                                                                                                                   <  8:191764/148‑1 (MQ=255)
                          gatgaGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGt                                                                                                                                >  1:133786/1‑134 (MQ=255)
                          gatgaGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGt                                                                                                                                <  2:133786/134‑1 (MQ=255)
                              agaCGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCa                                                                                                              >  7:97778/1‑148 (MQ=255)
                                 cgctcgctGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcgaag                                                                                                                                    >  3:212288/1‑123 (MQ=255)
                                 cgctcgctGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcgaag                                                                                                                                    <  4:212288/123‑1 (MQ=255)
                                  gctcgctGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCCCGa                                                                                                           >  1:153193/1‑147 (MQ=255)
                                   ctcgctGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcgaag                                                                                                                                    >  6:185518/1‑121 (MQ=255)
                                   ctcgctGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcgaag                                                                                                                                    <  5:185518/121‑1 (MQ=255)
                                   ctcgctGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGata                                                                                                         <  1:16343/148‑1 (MQ=255)
                                    tcgctGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGatat                                                                                                        >  8:227396/1‑148 (MQ=255)
                                      gctGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGatat                                                                                                        <  7:227396/146‑1 (MQ=255)
                                       ctGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCatat                                                                                                                                                  <  8:180874/103‑1 (MQ=255)
                                       ctGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCatat                                                                                                                                                  >  7:180874/1‑103 (MQ=255)
                                       ctGTCGGCCGCCACTGGGATAACATCTAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTTTTCGCGACGCTCTGACCCGTAATCCGGGTATCCTCCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGGACGATAtttt                                                                                                     <  4:276550/148‑1 (MQ=255)
                                          tcgtcCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCTCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCg                                                                                                   <  8:323841/147‑1 (MQ=255)
                                                   aCTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGtt                                                                                         <  6:26333/148‑1 (MQ=255)
                                                    cTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCGCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTg                                                                                        <  4:21491/148‑1 (MQ=255)
                                                     tGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTgg                                                                                       >  5:137184/1‑148 (MQ=255)
                                                      gggATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCGTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGt                                                                                      >  4:230714/1‑148 (MQ=255)
                                                      gggATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCGTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGt                                                                                      >  4:234757/1‑148 (MQ=255)
                                                      gggATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGAc                                                                                                                   <  6:51476/119‑1 (MQ=255)
                                                      gggATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGAc                                                                                                                   >  5:51476/1‑119 (MQ=255)
                                                       ggATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTgg                                                                                       <  8:97778/146‑1 (MQ=255)
                                                        gATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTc                                                                                    <  5:234436/148‑1 (MQ=255)
                                                          tAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAg                                                                                  >  7:147406/1‑148 (MQ=255)
                                                             cATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCGTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTAt                                                                               <  3:234757/148‑1 (MQ=255)
                                                             cATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCGTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTAt                                                                               <  3:230714/148‑1 (MQ=255)
                                                               tCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGc                                                                                                               >  3:106413/1‑114 (MQ=255)
                                                               tCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGc                                                                                                               <  4:106413/114‑1 (MQ=255)
                                                               tCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGt                                                                                      >  7:183469/1‑139 (MQ=255)
                                                               tCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGt                                                                                      <  8:183469/139‑1 (MQ=255)
                                                               tCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATcg                                                                             >  5:12749/1‑148 (MQ=255)
                                                               tCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATcg                                                                             >  1:218062/1‑148 (MQ=255)
                                                               tCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATcg                                                                             >  2:560/1‑148 (MQ=255)
                                                                cGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcga                                                                                                                                      <  7:112810/90‑1 (MQ=255)
                                                                cGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcga                                                                                                                                      >  8:112810/1‑90 (MQ=255)
                                                                cGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATcgc                                                                            >  4:193051/1‑148 (MQ=255)
                                                                   aGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGAt                                                                         <  3:193051/148‑1 (MQ=255)
                                                                     ttCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCa                                                                       >  5:251642/1‑148 (MQ=255)
                                                                       cgcgCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAg                                                                      <  8:3931/147‑1 (MQ=255)
                                                                          gcAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTg                                                                   >  8:312676/1‑147 (MQ=255)
                                                                            aaaaGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTgg                                                                  >  5:235791/1‑146 (MQ=255)
                                                                            aaaaGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGaa                                                                <  6:137184/148‑1 (MQ=255)
                                                                             aaaGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAg                                                               <  8:147406/148‑1 (MQ=255)
                                                                                      aaCCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATATTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAAcc                                                       >  4:153376/1‑147 (MQ=255)
                                                                                         cAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCt                                                   >  1:189340/1‑148 (MQ=255)
                                                                                             gTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGt                                               <  8:147592/148‑1 (MQ=255)
                                                                                                     aTTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTc                                                    <  3:138554/135‑1 (MQ=255)
                                                                                                     aTTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTc                                                    >  4:138554/1‑135 (MQ=255)
                                                                                                       tCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTgg                                                                  >  7:247223/1‑119 (MQ=255)
                                                                                                       tCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTgg                                                                  <  8:247223/119‑1 (MQ=255)
                                                                                                             cGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGt                               <  7:312676/148‑1 (MQ=255)
                                                                                                                    aCCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAAc                        <  1:153720/148‑1 (MQ=255)
                                                                                                                          aTTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGAtt                  >  2:39471/1‑148 (MQ=255)
                                                                                                                               gggTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGAtttt                >  3:264838/1‑145 (MQ=255)
                                                                                                                               gggTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGAtttt                <  4:264838/145‑1 (MQ=255)
                                                                                                                                  tATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCg          <  6:12749/148‑1 (MQ=255)
                                                                                                                                     ccaccaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTc           >  3:35178/1‑144 (MQ=255)
                                                                                                                                     ccaccaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTc           <  4:35178/144‑1 (MQ=255)
                                                                                                                                     ccaccaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGa         <  6:254404/146‑1 (MQ=255)
                                                                                                                                     ccaccaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAACGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGa         >  5:254404/1‑146 (MQ=255)
                                                                                                                                     ccaccaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCACCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGAGGAAAACATGAGTTTAGCTCGAt        >  6:2446/1‑147 (MQ=255)
                                                                                                                                       accaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGAt        >  3:63719/1‑145 (MQ=255)
                                                                                                                                       accaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGAt        <  4:63719/145‑1 (MQ=255)
                                                                                                                                       accaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGa     <  1:39471/148‑1 (MQ=255)
                                                                                                                                        ccaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGAACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGAt    >  7:115879/1‑148 (MQ=255)
                                                                                                                                        ccaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACAAGCACGATATTTTCGAGAAAGCGTTGGGTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACACGTGCAGCGCCGTGGCAAACATGATTTTAGCTCGCTTGAt    >  4:162765/1‑148 (MQ=255)
                                                                                                                                         caTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGAtg   >  7:89960/1‑148 (MQ=255)
                                                                                                                                          atatTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGAtgt  >  5:173279/1‑148 (MQ=255)
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ACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGT  >  NC_000913/441634‑441919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: