Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 464,113 C→T 5.2% G59G (GGC→GGT)  ybaV → putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913464,1130CT 5.2% 253.9 / 2.8 76G59G (GGC→GGT) ybaVputative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (34/38);  new base T (2/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01

ACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGG  >  NC_000913/463967‑464259
                                                                                                                                                  |                                                                                                                                                  
aCCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGc                                                                                                                                                    <  7:48225/147‑1 (MQ=255)
     gTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCgg                                                                                                                                              >  1:284477/1‑148 (MQ=255)
      tCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCgg                                                                                                                                              <  4:123554/147‑1 (MQ=255)
      tCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCg                                                                                                                                               <  2:59572/146‑1 (MQ=255)
        ccTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGCCGAAGAAGGCACCCGGGt                                                                                                                                            >  4:94766/1‑147 (MQ=255)
          tGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAg                                                                                                                                         >  4:109617/1‑148 (MQ=255)
            gCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAg                                                                                                                                         <  3:193054/146‑1 (MQ=255)
            gCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGc                                                                                                                                        <  2:258722/147‑1 (MQ=255)
            gCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGc                                                                                                                                        <  3:180522/147‑1 (MQ=255)
            gCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCa                                                                                                                                       <  3:222581/148‑1 (MQ=255)
                gtgCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATta                                                                                                                                    >  7:99561/1‑147 (MQ=255)
                 tgCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATta                                                                                                                                    <  3:109617/146‑1 (MQ=255)
                 tgCCGGAATGTCTCATAGCGCGCTGGAGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGATCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCAAGTGACGAAGAAGGCACCCGGGTCAGCATtaa                                                                                                                                   >  6:303292/1‑147 (MQ=255)
                  gCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATtaat                                                                                                                                  >  1:109529/1‑147 (MQ=255)
                   ccGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATtaataa                                                                                                                                <  3:212625/148‑1 (MQ=255)
                          gTCTCATAGCGCGCTGGCTGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATtaata                                                                                                                                 <  2:290790/140‑1 (MQ=255)
                          gTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATtaata                                                                                                                                 >  1:290790/1‑140 (MQ=255)
                              cATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATta                                                                                                                                    <  7:62610/133‑1 (MQ=255)
                              cATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATta                                                                                                                                    >  8:62610/1‑133 (MQ=255)
                              cATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGcgcg                                                                                                                      >  8:314319/1‑147 (MQ=255)
                               aTAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCgg                                                                                                                     >  4:13295/1‑147 (MQ=255)
                               aTAGCGCGCGGGCGGCAGCTTCTGGGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTCCCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGa                                                                                                                    <  6:7753/148‑1 (MQ=255)
                                  tcgcggTGGCGGCCGCTTCTGTTGGGAAACCGTCGCCGGTAGAAACCACAGCGGAAGCTCCTCCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCCCCCGGGTCAGCATTAATAATGCGAGCGCGGAag                                                                                                                  <  1:268478/146‑1 (MQ=255)
                                  gcgcgcTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGaa                                                                                                                   >  3:62128/1‑146 (MQ=255)
                                  gcgcgcTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAag                                                                                                                  <  7:106945/147‑1 (MQ=255)
                                            ggcAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCcgc                                                                                                       <  2:298659/148‑1 (MQ=255)
                                                   tCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATg                                                                                                 >  5:115724/1‑147 (MQ=255)
                                                    cTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGaa                                                                                               >  2:102879/1‑148 (MQ=255)
                                                       tgGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAAt                                                                                              <  8:56150/146‑1 (MQ=255)
                                                          cGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGt                                                                                         <  1:158785/148‑1 (MQ=255)
                                                             aaCCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATtaata                                                                                                                                 <  1:158499/105‑1 (MQ=255)
                                                             aaCCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATtaata                                                                                                                                 >  2:158499/1‑105 (MQ=255)
                                                             aaCCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTgg                                                                                      <  4:160183/148‑1 (MQ=255)
                                                                 gACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCAtt                                                                                                                                     <  3:77655/97‑1 (MQ=255)
                                                                 gACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCAtt                                                                                                                                     >  4:77655/1‑97 (MQ=255)
                                                                  aCGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGCTGAATGGCGTTGGCCTg                                                                                  >  3:97489/1‑147 (MQ=255)
                                                                  aCGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTga                                                                                 <  6:30778/148‑1 (MQ=255)
                                                                  aCGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTga                                                                                 <  3:3756/148‑1 (MQ=255)
                                                                      cggTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTgaagaa                                                                             <  2:284477/148‑1 (MQ=255)
                                                                        gTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAg                                                                           <  4:256009/148‑1 (MQ=255)
                                                                          aGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAag                                                                                                                  >  6:56589/1‑107 (MQ=255)
                                                                          aGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAag                                                                                                                  <  5:56589/107‑1 (MQ=255)
                                                                          aGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGc                                                                                           <  8:302897/130‑1 (MQ=255)
                                                                          aGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGc                                                                                           >  7:302897/1‑130 (MQ=255)
                                                                          aGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGAAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGGATGGCGTTGGCCTGAAGAAAgcg                                                                         >  5:316661/1‑148 (MQ=255)
                                                                               ccAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGc                                                                    >  8:283668/1‑148 (MQ=255)
                                                                                           cTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCa                                                            <  4:292759/144‑1 (MQ=255)
                                                                                           cTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCa                                                            >  3:292759/1‑144 (MQ=255)
                                                                                              cTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATcg                                                     <  3:13295/148‑1 (MQ=255)
                                                                                                  agcaCAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGTACCCGGGTCAGCAtt                                                                                                                                     >  7:164223/1‑64 (MQ=255)
                                                                                                  agcaCAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGTACCCGGGTCAGCAtt                                                                                                                                     <  8:164223/64‑1 (MQ=255)
                                                                                                  agcaCAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGTACCCGGGTCAGCAtt                                                                                                                                     <  6:74891/64‑1 (MQ=255)
                                                                                                  agcaCAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGTACCCGGGTCAGCAtt                                                                                                                                     >  5:74891/1‑64 (MQ=255)
                                                                                                   gcaCAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAag                                                <  6:115724/148‑1 (MQ=255)
                                                                                                   gcaCAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAag                                                <  7:314319/148‑1 (MQ=255)
                                                                                                      caAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGt                                             <  2:109529/148‑1 (MQ=255)
                                                                                                                cagcagTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGTGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATTAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCa                                                            <  6:245440/123‑1 (MQ=255)
                                                                                                                cagcagTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCa                                                            >  5:245440/1‑123 (MQ=255)
                                                                                                                   cagTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCTGGTCATCATTAATAATGCCAGAGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGATGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTTCg                                          <  6:265648/138‑1 (MQ=255)
                                                                                                                    agTACCCGCGAAAGACAGTGACTAAGAAGGCACCCGGTTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCTCGCGATGAATCGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACgg                                         >  5:265648/1‑138 (MQ=255)
                                                                                                                      tACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAAc                             <  4:62128/148‑1 (MQ=255)
                                                                                                                       aCCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGt                                             >  8:8652/1‑131 (MQ=255)
                                                                                                                       aCCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGt                                             <  7:8652/131‑1 (MQ=255)
                                                                                                                       aCCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACt                            >  4:36825/1‑148 (MQ=255)
                                                                                                                       aCCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACt                            >  4:13326/1‑148 (MQ=255)
                                                                                                                        ccGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACt                            <  1:102879/147‑1 (MQ=255)
                                                                                                                          ggCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGATGAGTACGGTCCGTTTAAAACtgtg                         >  6:58603/1‑148 (MQ=255)
                                                                                                                          ggCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACtgtg                         <  7:283668/148‑1 (MQ=255)
                                                                                                                                aGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTgg                        <  5:85486/143‑1 (MQ=255)
                                                                                                                                aGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCCCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTgg                        >  6:85486/1‑143 (MQ=255)
                                                                                                                                    aGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTa                >  6:134555/1‑147 (MQ=255)
                                                                                                                                       gACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTggagga                    <  5:266185/140‑1 (MQ=255)
                                                                                                                                       gACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTggagga                    >  6:266185/1‑140 (MQ=255)
                                                                                                                                         cGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAg          >  2:301514/1‑148 (MQ=255)
                                                                                                                                         cGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAg          >  7:315466/1‑148 (MQ=255)
                                                                                                                                                 gCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCggg  >  8:211073/1‑148 (MQ=255)
                                                                                                                                                 gCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCggg  >  4:47640/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACCCGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGG  >  NC_000913/463967‑464259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑