Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 604,258 G→A 5.0% P136S (CCG→TCG)  ybdG ← mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913604,2580GA 5.0% 263.8 / 2.7 79P136S (CCG→TCG) ybdGmechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base G (39/36);  new base A (2/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

CCAACATCAGCACGGCAGCCATTGCACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAGCCAGAA  >  NC_000913/604120‑604405
                                                                                                                                          |                                                                                                                                                   
ccAACATCAGCACGGCAGCCATTGCACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTgag                                                                                                                                             <  5:173389/147‑1 (MQ=255)
ccAACATCAGCACGGCAGCCATTGCACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTgag                                                                                                                                             <  2:255651/147‑1 (MQ=255)
ccAACATCAGCACGGCAGCCATTGCACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTgag                                                                                                                                             <  5:240404/147‑1 (MQ=255)
ccAACATCAGCACGGCAGCCATTGCACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTgag                                                                                                                                             <  7:172337/147‑1 (MQ=255)
   aCATCAGCACGGCAGCCATTGCACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGAtgc                                                                                                                                         <  4:87184/148‑1 (MQ=255)
       cAGCACGGCAGCCATTGCACCAAGCCCGCTGATCAGAATCGCTGGCGCCTGACCAATCAGCAGAGAGATCATCAAAATGCCGACAAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGcc                                                                                                                                     >  2:271098/1‑148 (MQ=255)
               cAGCCATTGCACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAAttt                                                                                                                             >  6:294747/1‑148 (MQ=255)
                      tGCACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCg                                                                                                                                    >  4:262362/1‑134 (MQ=255)
                      tGCACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTgcgc                                                                                                                       >  3:234737/1‑147 (MQ=255)
                      tGCACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAAGCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCg                                                                                                                                    <  3:262362/134‑1 (MQ=255)
                       gCACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTgcgc                                                                                                                       >  2:173167/1‑146 (MQ=255)
                             aGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGAGCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTgag                                                                                                                                             <  1:103775/118‑1 (MQ=255)
                              gACCGCTGATAAGAAACGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGACGATCAGTTTAATCCCCTGAAAAATCCCTTTCACCGGTAACTgaga                                                                                                                                            >  2:103775/1‑118 (MQ=255)
                                 cGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCAGATCAGTTTAATCCACTTAAATATCCATTTCAGCGGTAACTGAGATGATGCAGGGAATTTCTGCTCAAGATTCaaaa                                                                                                            >  8:310574/1‑147 (MQ=255)
                                  gCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAATTGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAAt                                                                                                           >  8:255674/1‑147 (MQ=255)
                                  gCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAAt                                                                                                           >  7:202859/1‑147 (MQ=255)
                                   cTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATaa                                                                                                         >  7:126537/1‑148 (MQ=255)
                                       tCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTgaga                                                                                                                                            <  2:299976/109‑1 (MQ=255)
                                       tCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTgaga                                                                                                                                            >  1:299976/1‑109 (MQ=255)
                                           aaTCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCATTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATc                                                                                                     <  1:21789/144‑1 (MQ=255)
                                            aTCGCTGGCGACTTACGAATCAGCAGCGAGATCATCAAAATGCCGACCATAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCa                                                                                                <  8:296639/148‑1 (MQ=255)
                                              cGCTGGCGACTGCCCAATCAGCAGCGAGATCATCCAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAAGATCCAGCAAc                                                                                              >  2:46835/1‑148 (MQ=255)
                                                  ggCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAACTATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACgaga                                                                                          <  4:231635/148‑1 (MQ=255)
                                                    ctaCTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGATAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATc                                                                                                     <  7:164025/133‑1 (MQ=255)
                                                    ccaCTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGATAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATc                                                                                                     <  7:159458/133‑1 (MQ=255)
                                                    cGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGATAACTGATATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATc                                                                                                     >  8:159458/1‑135 (MQ=255)
                                                    cGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGATAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATc                                                                                                     >  8:164025/1‑135 (MQ=255)
                                                      aCTGCCCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAAc                                                                                                        >  5:74691/1‑130 (MQ=255)
                                                      aCTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAAc                                                                                                        <  6:74691/130‑1 (MQ=255)
                                                      aCTGACCAATCAGCAGC‑‑GATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTgag                                                                                                                                             <  3:262756/91‑1 (MQ=255)
                                                      aCTGACCAATCAGCAGC‑‑GATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTgag                                                                                                                                             >  4:262756/1‑91 (MQ=255)
                                                         gACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTg                                                                                    >  7:212967/1‑147 (MQ=255)
                                                           ccAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGa                                                                                   >  5:544/1‑146 (MQ=255)
                                                             aaTCAGCATCTAGATCATAAAAATGCCGACCAGAATCGCGCAGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGTGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACTAGAAGACTGAAagc                                                                               <  7:310574/148‑1 (MQ=255)
                                                              aTCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAagca                                                                              <  4:294582/148‑1 (MQ=255)
                                                              aTCAGCAGCGAGATCATCAAAATGCCGACCAGAAACGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAag                                                                                >  2:275606/1‑146 (MQ=255)
                                                               tCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAACAACGAGAAGACTGAAagcag                                                                             >  6:114188/1‑148 (MQ=255)
                                                                cagcagCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAAc                                                                                                        <  4:258484/120‑1 (MQ=255)
                                                                cagcagCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAAc                                                                                                        >  3:258484/1‑120 (MQ=255)
                                                                cagcagCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAagcagc                                                                            >  4:199896/1‑148 (MQ=255)
                                                                      cGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTgag                                                                                                                                             <  8:322108/77‑1 (MQ=255)
                                                                      cGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTgag                                                                                                                                             >  7:322108/1‑77 (MQ=255)
                                                                      cGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATAc                                                                      <  5:294747/148‑1 (MQ=255)
                                                                             atcaAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGa                                                               <  6:544/148‑1 (MQ=255)
                                                                                         aCCAGAATCGCTCCGATCAGTTTAATCCCCTGAAATATCCCTTTAATCTGTAACTGAGATGCTGACGGTAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTTAAAGCAGCGCATACATCATGATCCACAACTgcg                                                   <  3:209243/148‑1 (MQ=255)
                                                                                          ccAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGATTTTCTGCGCCAGATTCAAAATAACAGCCAGCAACGAGAAGCGTGAAAGCAGCCGATCCAGCATGATCCACCACTccgc                                                  >  6:239337/1‑148 (MQ=255)
                                                                                                   cgcCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGt                                         >  2:9370/1‑148 (MQ=255)
                                                                                                    gcCGATCATTTTAATCCCCTGAAATATCCCTTTCAGAGGTAACTGAGATGATGCCGGGAATTTCTGCTCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAgcgc                                                                          <  4:157383/114‑1 (MQ=255)
                                                                                                    gcCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTc                                        <  3:199896/148‑1 (MQ=255)
                                                                                                     ccGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCa                                                                         >  3:157383/1‑114 (MQ=255)
                                                                                                        aTCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGaa                                    >  8:219060/1‑148 (MQ=255)
                                                                                                         tCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGaa                                    >  4:43723/1‑147 (MQ=255)
                                                                                                          cAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGAGCCAGATTCAAAATAACATCCAGAAACGAGAAGACTGAAATCAGCGCATACATCATGATCCGCaa                                                        >  1:308940/1‑126 (MQ=255)
                                                                                                           aGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAata                                  <  8:212967/147‑1 (MQ=255)
                                                                                                           aGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAata                                  <  1:173167/147‑1 (MQ=255)
                                                                                                           aGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAata                                  <  8:126537/147‑1 (MQ=255)
                                                                                                             tttAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACACCTGCGCGCAGGTAGTCAGAATATCt                               >  5:121998/1‑148 (MQ=255)
                                                                                                                 aTCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCg                           >  5:40257/1‑148 (MQ=255)
                                                                                                                    cccTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAACAACGAGAAGACTGAAAGCAGCGCATACATCATTATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCt                         <  5:114188/147‑1 (MQ=255)
                                                                                                                     gcTGATATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCGAGCAACGAGAAGACTGAAAGGAGCGCATACATCATGATCCACTACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTc                       <  1:275606/147‑1 (MQ=255)
                                                                                                                     ccTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTc                       >  8:130042/1‑148 (MQ=255)
                                                                                                                      cTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCg                      >  5:177236/1‑148 (MQ=255)
                                                                                                                        gAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGt                    >  7:160587/1‑148 (MQ=255)
                                                                                                                        gAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGt                    >  1:60305/1‑148 (MQ=255)
                                                                                                                         aaaTATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGcc                            <  1:18894/139‑1 (MQ=255)
                                                                                                                         aaaTATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGcc                            >  2:18894/1‑139 (MQ=255)
                                                                                                                         aaaTATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTg                   <  8:202859/148‑1 (MQ=255)
                                                                                                                          aaTATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCcaca                                                         >  1:58699/1‑109 (MQ=255)
                                                                                                                          aaTATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCcaca                                                         <  2:58699/109‑1 (MQ=255)
                                                                                                                             atCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTgcgcgc                                                <  8:90186/115‑1 (MQ=255)
                                                                                                                             atCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTgcgcgc                                                >  7:90186/1‑115 (MQ=255)
                                                                                                                              tCCCATTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCttt              <  5:239337/148‑1 (MQ=255)
                                                                                                                                ccTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCttttt            <  6:40257/148‑1 (MQ=255)
                                                                                                                                ccTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCttttt            <  7:255674/148‑1 (MQ=255)
                                                                                                                                ccTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCtttt             <  1:9370/147‑1 (MQ=255)
                                                                                                                                 cTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTg           >  3:136439/1‑148 (MQ=255)
                                                                                                                                  tttCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTg           >  8:262161/1‑147 (MQ=255)
                                                                                                                                   ttCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTg           >  8:284469/1‑146 (MQ=255)
                                                                                                                                    tCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGc          <  8:160587/146‑1 (MQ=255)
                                                                                                                                         ggTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAGCCAGa   <  7:262161/148‑1 (MQ=255)
                                                                                                                                          gTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAGCCAGaa  >  5:185973/1‑148 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
CCAACATCAGCACGGCAGCCATTGCACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAGCCAGAA  >  NC_000913/604120‑604405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: