Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 886,972 G→A 25.8% A123V (GCG→GTG)  ybjJ ← putative drug efflux MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913886,9720GA25.8% 151.1 / 54.6 86A123V (GCG→GTG) ybjJputative drug efflux MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (32/31);  new base A (9/13);  total (41/45)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

CCGTTGCCGGAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAA  >  NC_000913/886832‑887119
                                                                                                                                            |                                                                                                                                                   
ccGTTGCCGGAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAACACCGTGCATCATCGGCAAAACCGTCGTATTCATTTCTCGCTCACCGGCAGCACCTTCAACGTTTATCGCCACTTc                                                                                                                                              >  5:210743/1‑148 (MQ=255)
ccGTTGCCGGAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTc                                                                                                                                              >  3:244369/1‑148 (MQ=255)
     gCCGGAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCa                                                                                                                                          <  2:66948/147‑1 (MQ=255)
      ccGGAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAg                                                                                                                                         >  1:77045/1‑147 (MQ=255)
         gAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAAc                                                                                                                                      >  2:207820/1‑147 (MQ=255)
           aCGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCaa                                                                                                                                   <  1:38606/148‑1 (MQ=255)
            cGCCAAAGGCCGTCTGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGGTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCaaa                                                                                                                                  <  1:289710/148‑1 (MQ=255)
            cGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCaaa                                                                                                                                  <  8:104705/148‑1 (MQ=255)
             gCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCaaaa                                                                                                                                 <  7:139101/148‑1 (MQ=255)
                aaaGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACtt                                                                                                                              >  2:323907/1‑148 (MQ=255)
                         cAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCa                                                                                                                        >  7:307377/1‑145 (MQ=255)
                            tGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACg                                                                                                                  <  6:25978/148‑1 (MQ=255)
                            tGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACg                                                                                                                  >  5:25978/1‑148 (MQ=255)
                             gCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCaaa                                                                                                                                  >  8:104712/1‑131 (MQ=255)
                             gCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCaaa                                                                                                                                  <  7:104712/131‑1 (MQ=255)
                             gCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGc                                                                                                                 >  2:63980/1‑148 (MQ=255)
                               cATCCCGTCACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCGCCAAAGACGCCg                                                                                                               <  8:330054/148‑1 (MQ=255)
                               cATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCg                                                                                                               <  4:10851/148‑1 (MQ=255)
                                aTCCCGACACCTGAGCATGCCAGCGTGCCCAGGCTATAAAAACCGTGCCTCATCGGCAAACCCGTCTTATGCATTTCTCCCTCAACGGCACCACCTTCAACGATTATCACCACTTCCGCACAACCAAAACTTGCCCCAAACACGcc                                                                                                                >  2:201010/1‑146 (MQ=255)
                                   ccGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGAc                                                                                                           >  6:288663/1‑148 (MQ=255)
                                     gACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACtt                                                                                                                              >  3:279852/1‑127 (MQ=255)
                                     gACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACtt                                                                                                                              <  4:279852/127‑1 (MQ=255)
                                       cacCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCaaa                                                                                                                                  >  5:103447/1‑121 (MQ=255)
                                       cacCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCaaa                                                                                                                                  <  6:103447/121‑1 (MQ=255)
                                           tGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGc                                                                                                    >  5:85979/1‑147 (MQ=255)
                                             cgcCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCaa                                                                                                  <  2:141901/147‑1 (MQ=255)
                                              gcCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGTCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAAGGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAag                                                                                                <  1:201010/148‑1 (MQ=255)
                                                  gCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAagag                                                                                              >  3:234077/1‑146 (MQ=255)
                                                  gCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCa                                                                                            <  4:2545/148‑1 (MQ=255)
                                                    cAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAgg                                                                                          <  1:207820/148‑1 (MQ=255)
                                                      gCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAgggg                                                                                        <  6:85979/148‑1 (MQ=255)
                                                        gTGGCCGGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCGCAACGGCCGCACCTTCATCGGTTATCTCCACTTCCGCAGAACCAACAGTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCCGGGGCg                                                                                      <  6:284455/148‑1 (MQ=255)
                                                        gTGCCCATGCGATAAAAACCGTTCATAATCGTCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCATGGGCg                                                                                      <  6:280030/148‑1 (MQ=255)
                                                        gTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCg                                                                                      <  1:63980/148‑1 (MQ=255)
                                                        gTGCCCACGCTATAAACACCGTGCATCATCGGCAAACCCGTCTTATTCATTTCTCGCGCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCCCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCg                                                                                      <  6:210743/148‑1 (MQ=255)
                                                           cccAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATg                                                                                   >  8:212063/1‑148 (MQ=255)
                                                             cAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGt                                                                                  >  4:99610/1‑147 (MQ=255)
                                                              aGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGc                                                                                                                            <  2:253086/104‑1 (MQ=255)
                                                              aGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGc                                                                                                                            >  1:253086/1‑104 (MQ=255)
                                                              aGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGt                                                                                  >  6:250057/1‑146 (MQ=255)
                                                              aGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCaa                                                                                                  >  1:10/1‑130 (MQ=255)
                                                              aGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCaa                                                                                                  <  2:10/130‑1 (MQ=255)
                                                               ggCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAg                                                                               <  2:77045/148‑1 (MQ=255)
                                                               ggCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAg                                                                               >  4:68521/1‑148 (MQ=255)
                                                                gCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGc                                                                              >  4:251647/1‑148 (MQ=255)
                                                                 cTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTGAGcc                                                                             <  5:288663/148‑1 (MQ=255)
                                                                    taAAAACCGTGCATCATCGGCAAAACCGTCTTATTCAAGTCTCGCTCAACGGCACCACCTTCAACGTTTATCGCCCCTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGATCCCAACGGCAAAGAGCACGGCCGATGGCAGCCa                                                                            >  1:66645/1‑146 (MQ=255)
                                                                     aaaaaCCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCagag                                                                         >  3:19497/1‑148 (MQ=255)
                                                                      aaaaCCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCagag                                                                         <  1:323907/147‑1 (MQ=255)
                                                                        aaCCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGc                                                                      <  8:12000/148‑1 (MQ=255)
                                                                        aaCCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGc                                                                      >  7:77387/1‑148 (MQ=255)
                                                                         aCCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGcc                                                                     <  8:307377/148‑1 (MQ=255)
                                                                           cGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGc                                                                      >  2:28186/1‑145 (MQ=255)
                                                                            gTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGa                                                                  >  5:203439/1‑148 (MQ=255)
                                                                               catcatCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACtt                                                               <  8:77387/148‑1 (MQ=255)
                                                                                 tcatcGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTaa                                                             <  4:244369/148‑1 (MQ=255)
                                                                                  catcGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTaa                                                             >  8:324215/1‑147 (MQ=255)
                                                                                  catcGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTa                                                              >  3:298393/1‑146 (MQ=255)
                                                                                  catcGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTa                                                              <  4:298393/146‑1 (MQ=255)
                                                                                  catcGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGc                                                                                             >  7:77838/1‑115 (MQ=255)
                                                                                  catcGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGc                                                                                             <  8:77838/115‑1 (MQ=255)
                                                                                  catcGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTaa                                                             >  3:163151/1‑147 (MQ=255)
                                                                                     cGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAg                                                                               >  8:174233/1‑126 (MQ=255)
                                                                                     cGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAg                                                                               <  7:174233/126‑1 (MQ=255)
                                                                                      ggCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatc                                                         <  1:192859/147‑1 (MQ=255)
                                                                                       gCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCCGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatcat                                                       <  5:250057/148‑1 (MQ=255)
                                                                                        cAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatcatc                                                      <  3:251647/148‑1 (MQ=255)
                                                                                        cAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatcat                                                       >  1:177920/1‑147 (MQ=255)
                                                                                          aaaCCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatcatcat                                                    <  7:324215/148‑1 (MQ=255)
                                                                                               gTCTTATTCATTTCTCGCTCAACGGCAGCAGCTTCAAAGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCACATAGACGACGAGACCAACGGCAAAGAGCAGTTGCGATGTCAGCCAGAGTGCCAGACTTAATatc                                                         <  8:206046/138‑1 (MQ=255)
                                                                                                tCTTATTCATTTCTCGCTCAACGGCAGCACCTTCACCGTTTATAGCCACTTCCGCAGAACCAAAAATTGCACCAAAGACGCCGAGACAAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatca                                                        >  7:206046/1‑138 (MQ=255)
                                                                                                         tttCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCa                                     <  6:203439/148‑1 (MQ=255)
                                                                                                             tcGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGAt                                              >  1:7594/1‑135 (MQ=255)
                                                                                                             tcGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGAt                                              <  2:7594/135‑1 (MQ=255)
                                                                                                              cGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACa                                >  8:93238/1‑148 (MQ=255)
                                                                                                               gCTCAACGGCAGCACCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGACAGACTTAATATCATCATCCCGATCAATGCGCAGGAc                                 <  3:68521/146‑1 (MQ=255)
                                                                                                                  cAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAgg                                   <  3:34074/141‑1 (MQ=255)
                                                                                                                  cAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAgg                                   >  4:34074/1‑141 (MQ=255)
                                                                                                                  cAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGt                            >  4:174072/1‑148 (MQ=255)
                                                                                                                   aaCGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCg                             <  1:28186/146‑1 (MQ=255)
                                                                                                                     cGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGAc                         >  5:26992/1‑148 (MQ=255)
                                                                                                                            aCCTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATg                  <  4:19497/148‑1 (MQ=255)
                                                                                                                              cTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGAc                <  3:99610/148‑1 (MQ=255)
                                                                                                                              cTTCAACGTTTATCACCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGAc                <  4:163151/148‑1 (MQ=255)
                                                                                                                                    cGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACg          >  8:187500/1‑148 (MQ=255)
                                                                                                                                       ttATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTGATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACgtgt       >  2:121128/1‑148 (MQ=255)
                                                                                                                                            gCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCaa  <  7:187500/148‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
CCGTTGCCGGAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAA  >  NC_000913/886832‑887119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: