Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 996,051 C→T 6.7% V298V (GTG→GTA ssuA ← aliphatic sulfonate ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913996,0510CT 6.7% 181.3 / 2.4 59V298V (GTG→GTAssuAaliphatic sulfonate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (31/24);  new base T (2/2);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGC  >  NC_000913/995905‑996191
                                                                                                                                                  |                                                                                                                                            
aTCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGc                                                                                                                                              <  3:250359/147‑1 (MQ=255)
aTCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGc                                                                                                                                              <  6:274725/147‑1 (MQ=255)
  cAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCAc                                                                                                                                            >  4:74211/1‑147 (MQ=255)
  cAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCAc                                                                                                                                            >  7:51419/1‑147 (MQ=255)
    aCCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCAc                                                                                                                                            >  7:79736/1‑145 (MQ=255)
          cGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGAtt                                                                                                                                   >  5:235153/1‑148 (MQ=255)
                   tcttcCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATaaa                                                                                                                           >  3:188144/1‑147 (MQ=255)
                       ccGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAAc                                                                                                                         >  4:278064/1‑145 (MQ=255)
                       ccGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAAc                                                                                                                         <  3:278064/145‑1 (MQ=255)
                        cGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGa                                                                                                                      <  8:51419/147‑1 (MQ=255)
                        cGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGa                                                                                                                      <  6:235153/147‑1 (MQ=255)
                          ttCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCACTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCt                                                                                                                   >  7:132015/1‑148 (MQ=255)
                             ccAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGc                                                                                                                 <  5:66159/147‑1 (MQ=255)
                                gATAATGCCCGACACCGTGGGTCGGTAAAAACCAGAAAATATACAGACTCATAATTGTTTTCCTTCCAGTTGATTCGGCTCCCACATGCGCTGGCGAAAATCGACTTTTTTCGGCACCAGACGAATTACATAAAACAGATCAGCCgat                                                                                                             >  2:302349/1‑146 (MQ=255)
                                 aTAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGttt                                                                                                            <  5:79015/148‑1 (MQ=255)
                                    aTGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTtgct                                                                                                         >  7:319773/1‑148 (MQ=255)
                                       cccGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTtgctgc                                                                                                       <  8:79736/147‑1 (MQ=255)
                                       cccGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTgctgct                                                                                                      >  8:97821/1‑148 (MQ=255)
                                           tCACCGTGGGGCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTa                                                                                                   <  5:123916/147‑1 (MQ=255)
                                            cACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGTACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAg                                                                                                 >  6:84514/1‑148 (MQ=255)
                                            cACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGt                                                                                                    >  6:123916/1‑145 (MQ=255)
                                              ccGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGTACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAggc                                                                                               <  5:84514/148‑1 (MQ=255)
                                                gTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAg                                                                                                                       <  4:289374/122‑1 (MQ=255)
                                                gTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAg                                                                                                                       >  3:289374/1‑122 (MQ=255)
                                                      cGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAATGCGGCCACCt                                                                                       >  6:196946/1‑148 (MQ=255)
                                                         tAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATAGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCgg                                                                                    >  5:203805/1‑148 (MQ=255)
                                                            aaaCCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGt                                                                                 >  5:175646/1‑148 (MQ=255)
                                                               ccAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTaa                                                                              <  7:14571/148‑1 (MQ=255)
                                                                cAGAACATTTTCAGTCTCTTAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAAc                                                                             <  6:175646/148‑1 (MQ=255)
                                                                 aGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCTCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAAcc                                                                            <  8:132015/148‑1 (MQ=255)
                                                                   aaCATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCgg                                                                          >  1:187864/1‑148 (MQ=255)
                                                                       tatTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTg                                                                      <  8:319773/148‑1 (MQ=255)
                                                                          tCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTGAACCGCTTTGAtg                                                                   >  3:201128/1‑148 (MQ=255)
                                                                           cAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCgg                                                                                    <  1:92462/130‑1 (MQ=255)
                                                                           cAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCgg                                                                                    >  2:92462/1‑130 (MQ=255)
                                                                                  aTAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATATAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGAGTTAACCGGTTTGATGGTGGTa                                                             <  1:302349/146‑1 (MQ=255)
                                                                                        gTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGTACCAGACGATTTTCATAAAACAGAt                                                                                                                     <  7:164179/84‑1 (MQ=255)
                                                                                        gTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGTACCAGACGATTTTCATAAAACAGAt                                                                                                                     >  8:164179/1‑84 (MQ=255)
                                                                                                tccAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAAc                                                                                                                         >  8:60066/1‑72 (MQ=255)
                                                                                                tccAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAAc                                                                                                                         <  7:60066/72‑1 (MQ=255)
                                                                                                 ccAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATTTCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCTGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCta                                             <  5:196946/147‑1 (MQ=255)
                                                                                                    gTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCtaagt                                          >  7:12532/1‑147 (MQ=255)
                                                                                                     ttGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCtaagt                                          >  7:39822/1‑146 (MQ=255)
                                                                                                            ggCTGCCAGAGGCGCTGGAGAATATCGAGTGTTTTGGGCCCCAGCAGCTTTTCATAAACCAGATCTGCCGGTTGCTGCTGTAAGGCGGCAACCTTGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCTAAGTAAGAGGCa                                  <  2:71515/147‑1 (MQ=255)
                                                                                                                gCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCg                                                                                     <  3:222568/92‑1 (MQ=255)
                                                                                                                gCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCg                                                                                     >  4:222568/1‑92 (MQ=255)
                                                                                                                gCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGGTGATCTAAGTAAGAGGCAATCAc                             >  1:310684/1‑148 (MQ=255)
                                                                                                                  cAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGATGTAAGGCGGCACCCTCGGCGTTAACCGGTTTTATGGTGGTAGGAGGGCGATGATGTAAGTAAGAGGCTATCACCg                           >  6:6829/1‑148 (MQ=255)
                                                                                                                    gATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCtaagtaag                                       >  3:234278/1‑134 (MQ=255)
                                                                                                                    gATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCtaagtaag                                       <  4:234278/134‑1 (MQ=255)
                                                                                                                       gcgcTGGCGAAGATCGACTTTTTTCGGCACCATCCGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTACGGCGGCAACCTAGGCGTTAAGCGGTTTGATGGTGGTAGGTGGGAGAGGCGCGTAGGAAGAGGCAATCACCGGTGcc                      >  6:256679/1‑148 (MQ=255)
                                                                                                                          cTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGTCAACCTCGGAGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGt                   <  4:188144/148‑1 (MQ=255)
                                                                                                                              cGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCtaagt                                          >  7:2605/1‑121 (MQ=255)
                                                                                                                              cGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCtaagt                                          <  8:2605/121‑1 (MQ=255)
                                                                                                                                  tatCGAATTTTTTCGTCACCAGACGATTTTCATAAAACAGATCTGCCGTTTTCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCg                                                     <  2:44363/106‑1 (MQ=255)
                                                                                                                                   atCGACTTTTTTCGGCAACAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACAGGTTTGATGGTGGTAGGAGGGCga                                                    >  1:44363/1‑106 (MQ=255)
                                                                                                                                       aCTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCgg                                                                          <  7:229433/80‑1 (MQ=255)
                                                                                                                                       aCTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCgg                                                                          >  8:229433/1‑80 (MQ=255)
                                                                                                                                       aCTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGttt      <  6:203805/148‑1 (MQ=255)
                                                                                                                                          tttttCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCTAAGTAAGGGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTg   >  8:94366/1‑148 (MQ=255)
                                                                                                                                            tttCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGc  >  8:61327/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
ATCAACCGGGCGTGAACCTTCTTCCGTTCCCAGATAATGCCCGTCACCGTGGGTCGGTAAAAACCAGAACATATTCAGACTCATAATTGTTTTCCTTCCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGC  >  NC_000913/995905‑996191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: