Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,159,109 A→G 100% N414S (AAC→AGC)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,159,1090AG100.0% 177.0 / NA 48N414S (AAC→AGC) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/26);  total (22/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTAC  >  NC_000913/1158967‑1159250
                                                                                                                                              |                                                                                                                                             
gTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCAtt                                                                                                                                           <  3:91214/147‑1 (MQ=255)
gTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCAtt                                                                                                                                           <  3:102180/147‑1 (MQ=255)
  ttACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTAc                                                                                                                                         >  8:35894/1‑147 (MQ=255)
   tactacACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACt                                                                                                                                        >  3:96891/1‑147 (MQ=255)
     ctacACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGCCGCGACTGAAGATGCAAAAGCGACAGGTACCAGAGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTac                                                                                                                                      <  1:24530/147‑2 (MQ=255)
       acacCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTTAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCAATTGGTGGTAAATAAAGCATTACTAACCt                                                                                                                                   >  5:242145/1‑148 (MQ=255)
         acCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATTGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTc                                                                                                                                  >  3:73930/1‑147 (MQ=255)
          ccATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGa                                                                                                                                <  4:96891/148‑1 (MQ=255)
          ccATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGa                                                                                                                                >  4:215133/1‑148 (MQ=255)
                      tGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTAc                                                                                                                    >  4:2646/1‑148 (MQ=255)
                        cTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTAccc                                                                                                                  >  8:140843/1‑148 (MQ=255)
                          gATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGt                                                                                                                >  5:118449/1‑148 (MQ=255)
                           aTTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGt                                                                                                                <  6:301821/147‑1 (MQ=255)
                                      ggATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGc                                                                                                    >  4:276601/1‑148 (MQ=255)
                                            gAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCt                                                                                              >  4:182924/1‑148 (MQ=255)
                                             aaaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTg                                                                                                <  3:2646/145‑1 (MQ=255)
                                             aaaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTg                                                                                             <  3:44392/148‑1 (MQ=255)
                                               aaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                                                                           <  3:276601/148‑1 (MQ=255)
                                                 cGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAtgt                                                                                         <  4:73930/148‑1 (MQ=255)
                                                  gCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAtgtg                                                                                        >  1:266060/1‑148 (MQ=255)
                                                   ccGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATgtgt                                                                                       <  3:215133/148‑1 (MQ=255)
                                                    cGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGAGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGt                                                                                                  <  3:134215/136‑1 (MQ=255)
                                                     gggTCTTGAATACGCTACTGAAGATGCAAAAGCGACAGGTACAAGCGAAATGGAACCGGCTCTGGTTGCTGCATTTGGAGGTAAAGAAAGCATAACTAAACTCGACGCATGTATTACACGTCTGCGCGTCATCGtt                                                                                                 >  4:134215/1‑136 (MQ=255)
                                                          gTGAAGACGCGACTGAAGATGCAAAAGCGACAGTTACCAGCGAACTGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGGCAGCGTTGCTGATGTGTCTAAAGt                                                                                <  7:154558/148‑1 (MQ=255)
                                                            gAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTg                                                                                             <  2:15993/133‑1 (MQ=255)
                                                            gAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTg                                                                                             >  1:15993/1‑133 (MQ=255)
                                                             aaGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGa                                                                             <  3:230435/148‑1 (MQ=255)
                                                             aaGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGa                                                                             <  6:242145/148‑1 (MQ=255)
                                                                     aCTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTgg                                                                              >  8:264317/1‑139 (MQ=255)
                                                                     aCTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTgg                                                                              <  7:264317/139‑1 (MQ=255)
                                                                       tGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccggc                                                                   >  6:218834/1‑148 (MQ=255)
                                                                        gAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccggcc                                                                  >  1:265515/1‑148 (MQ=255)
                                                                               cAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTgaaga                                                            >  5:179393/1‑147 (MQ=255)
                                                                                aaaaGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGaaa                                                          >  2:30929/1‑148 (MQ=255)
                                                                                 aaaGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAAc                                                         >  1:295813/1‑148 (MQ=255)
                                                                                   aGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTg                                                       <  7:35894/148‑1 (MQ=255)
                                                                                            tACCAGCGAAATGGCACCGACTCTGGTTGCTGCAGTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTCCCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCcgcg                                              <  3:172848/148‑1 (MQ=255)
                                                                                               cAGCGAAATGGCACCGTCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTa                                                                                                                        <  2:1858/71‑1 (MQ=255)
                                                                                               cAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTa                                                                                                                        >  1:1858/1‑71 (MQ=255)
                                                                                                   gAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAg                                       <  6:179393/148‑1 (MQ=255)
                                                                                                                   tGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCt                         <  2:265515/146‑1 (MQ=255)
                                                                                                                     ggtgctgcATTTGGGGGTAAAGAAAGCATTACTCACCTCGACGCATGTATTACCCGGCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTgg                       <  5:48082/144‑1 (MQ=255)
                                                                                                                     gTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGTGGGCGTAGTGGTTGCTGGTTCTgg                       <  7:140843/146‑1 (MQ=255)
                                                                                                                         ctgcATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTg                                 <  7:152999/132‑1 (MQ=255)
                                                                                                                         ctgcATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTg                                 >  8:152999/1‑132 (MQ=255)
                                                                                                                                  gtggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGAtttt        <  2:295813/148‑1 (MQ=255)
                                                                                                                                    ggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg      >  7:79900/1‑148 (MQ=255)
                                                                                                                                        aaGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTAc  <  8:79900/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                             
GTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTAC  >  NC_000913/1158967‑1159250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑