Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,557,956 T→C 7.8% H28R (CAT→CGT)  ddpF ← D,D‑dipeptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,557,9560TC 7.8% 155.8 / 3.9 51H28R (CAT→CGT) ddpFD,D‑dipeptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base C (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CTGCCCGTGGCTCGGTTGCAGCATACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAACAGGCACAGCGCTGGTTGTTGTCACCACACGCTG  >  NC_000913/1557823‑1558098
                                                                                                                                     |                                                                                                                                              
cTGCCCGTGGCTCGGTTGCAGCATACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGt                                                                                                                                      >  4:265935/1‑144 (MQ=255)
cTGCCCGTGGCTCGGTTGCAGCATACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGt                                                                                                                                      <  3:265935/144‑1 (MQ=255)
        ggCTCGGTTGCAGCATACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGc                                                                                                                            >  7:138185/1‑146 (MQ=255)
             ggTTGCAGCATACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGtttt                                                                                                                     <  7:108545/148‑1 (MQ=255)
                        aCCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCgg                                                                                                           >  6:48699/1‑147 (MQ=255)
                          ccATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGa                                                                                                         >  5:280714/1‑147 (MQ=255)
                            aTTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAAt                                                                                                      <  2:20620/148‑1 (MQ=255)
                              tAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAAtt                                                                                                     >  4:153444/1‑147 (MQ=255)
                              tAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACACGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAAtt                                                                                                     >  2:98859/1‑147 (MQ=255)
                              tAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGACTGAATCGCAGACGATCCCTAAGGTTTCAACACGGCGGATCTGTAAATCAATACCAATAATGGCATGAACACGTTCCGTAGATTTACAAAGCCAGATTTTACGGGCCGGGAAAtt                                                                                                     >  2:103310/1‑147 (MQ=255)
                               aaaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTg                                                                                                    <  8:138185/147‑1 (MQ=255)
                                aaaGCTGTGCGAGTGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGa                                                                                                   <  3:78992/147‑1 (MQ=255)
                                aaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGat                                                                                                  <  3:70454/148‑1 (MQ=255)
                                aaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGa                                                                                                   >  3:192845/1‑147 (MQ=255)
                                aaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACACGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGat                                                                                                  <  1:98859/148‑1 (MQ=255)
                                aaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACACGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGat                                                                                                  <  1:103310/148‑1 (MQ=255)
                                  aGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGatat                                                                                                <  5:48699/148‑1 (MQ=255)
                                     tgtgCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCaa                                                                                                                              >  1:113446/1‑115 (MQ=255)
                                        gCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGt                                                                                                                                              <  4:174775/96‑1 (MQ=255)
                                        gCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGt                                                                                                                                              >  3:174775/1‑96 (MQ=255)
                                              gTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTa                                                                                    <  2:136050/148‑1 (MQ=255)
                                               tGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGCCGTCGCGTaa                                                                                   >  5:240572/1‑148 (MQ=255)
                                               tGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTaa                                                                                   >  5:90339/1‑148 (MQ=255)
                                                gCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGtt                                                                                                                                     >  7:196374/1‑97 (MQ=255)
                                                gCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGtt                                                                                                                                     <  8:196374/97‑1 (MQ=255)
                                                   tttGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGt                                                                                >  4:207855/1‑147 (MQ=255)
                                                    ttGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGtt                                                                               <  1:242936/147‑1 (MQ=255)
                                                       ccGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTtaat                                                                            <  8:281498/147‑1 (MQ=255)
                                                           aGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATACCgt                                                                       <  5:45647/148‑1 (MQ=255)
                                                                     gCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGaca                                                               <  6:280714/146‑1 (MQ=255)
                                                                          cgaTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATaa                                                        >  4:153157/1‑148 (MQ=255)
                                                                               ccTAAGGTTTCACCACTGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAAcctc                                                    <  8:5231/147‑1 (MQ=255)
                                                                                  aaGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCctgc                                                >  6:11820/1‑148 (MQ=255)
                                                                                  aaGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTACAACCTCctgc                                                >  4:16594/1‑148 (MQ=255)
                                                                                     gTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCtgctgc                                             <  8:310344/148‑1 (MQ=255)
                                                                                           ccacGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATAc                                       <  4:192845/148‑1 (MQ=255)
                                                                                                   gATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAAc                                                                         >  6:186532/1‑106 (MQ=255)
                                                                                                   gATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAAc                                                                         <  5:186532/106‑1 (MQ=255)
                                                                                                      cTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTAAGACACTTATAACCTCCTGCTGCGGATACCAACAGTca                              >  5:208768/1‑146 (MQ=255)
                                                                                                               aaTACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAACAGGCACAGCGCTGgtt                   <  3:153157/148‑1 (MQ=255)
                                                                                                                     aTTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCgg                                           <  7:75724/118‑1 (MQ=255)
                                                                                                                     aTTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCgg                                           >  8:75724/1‑118 (MQ=255)
                                                                                                                                gAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAACAGGCACAGCGCTGgttgt                 <  7:259768/133‑1 (MQ=255)
                                                                                                                                gAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAACAGGCACAGCGCTGgttgt                 >  8:259768/1‑133 (MQ=255)
                                                                                                                                gAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAACAGGCACAGCGCTGGTTGTTGTCACCACACGCTg  >  4:157243/1‑148 (MQ=255)
                                                                                                                                 aaCATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAACAGGCACAGCGCTgg                     >  6:286263/1‑128 (MQ=255)
                                                                                                                                 aaCATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAACAGGCACAGCGCTgg                     <  5:286263/128‑1 (MQ=255)
                                                                                                                                  aaaTGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAACAGGCACAGCGCTGgt                    <  7:168251/126‑1 (MQ=255)
                                                                                                                                   cATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTTCGGATACAAACAGGCACAGCGATGgtt                   >  8:168251/1‑128 (MQ=255)
                                                                                                                                   cATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAAc                                   <  3:200222/112‑1 (MQ=255)
                                                                                                                                   cATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAAc                                   >  4:200222/1‑112 (MQ=255)
                                                                                                                                     tGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAACAGGCACAGCGCTGGTTGTTGTc             >  6:49983/1‑132 (MQ=255)
                                                                                                                                     tGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAACAGGCACAGCGCTGGTTGTTGTc             <  5:49983/132‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                              
CTGCCCGTGGCTCGGTTGCAGCATACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAGCCAGTTTTTACGGGCCGGGAAATTGATATGGACGTCGCGTAACGTTAATAACGTGTCAGACACTTATAACCTCCTGCTGCGGATACCAACAGGCACAGCGCTGGTTGTTGTCACCACACGCTG  >  NC_000913/1557823‑1558098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑