Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,627,440 C→A 8.2% intergenic (‑60/‑77) dcp ← / → ydfG dipeptidyl carboxypeptidase II/NADP‑dependent 3‑hydroxy acid dehydrogenase; malonic semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,627,4400CA 8.2% 183.0 / 2.1 54intergenic (‑60/‑77)dcp/ydfGdipeptidyl carboxypeptidase II/NADP‑dependent 3‑hydroxy acid dehydrogenase; malonic semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base C (25/24);  new base A (2/3);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAGTGATGATTGGCAATTTGATCAAAATGGGGAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGG  >  NC_000913/1627296‑1627587
                                                                                                                                                |                                                                                                                                                   
gATAGTGATGATTGGCAATTTGATCAAAATGGGGAGCCAGGTACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTg                                                                                                                                                  >  7:304504/1‑148 (MQ=255)
     tgatgaTTGGCAATTTGATCAAAATGGGGAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATc                                                                                                                                             <  3:85268/148‑1 (MQ=255)
      gatgatTGGCAATTTGATCAAAATGGGGAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATc                                                                                                                                             >  8:335213/1‑147 (MQ=255)
       atgatTGGCAATTTGATCAAAATGGGGAGCCAGATACGGCAGTGTGCTGTGCACAAGGAAAGGATGCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGGTCCATAGCGTAGGCTTACTGATAACGGATTGCAAGATTGCACTcc                                                                                                                                            <  1:286116/147‑1 (MQ=255)
          atTGGCAATTTGATCAAAATGGGGAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGt                                                                                                                                         <  7:68557/147‑1 (MQ=255)
          atTGGCAATTTGATCAAAATGGGGAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGt                                                                                                                                         <  3:53743/147‑1 (MQ=255)
              gCAATTTGATCAAAATGGGGAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTAc                                                                                                                                    <  7:13710/148‑1 (MQ=255)
                    tGATCAAAATGGGGAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGct                                                                                                                               <  7:335213/147‑1 (MQ=255)
                       tCAAAATGGGGAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTa                                                                                                                           <  5:22417/148‑1 (MQ=255)
                                  aGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCGTCCCGTg                                                                                                                >  1:173720/1‑148 (MQ=255)
                                     cAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGTACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGc                                                                                                                        <  2:299863/137‑1 (MQ=255)
                                     cAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGc                                                                                                                        >  1:299863/1‑137 (MQ=255)
                                     cAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTa                                                                                                             <  5:141859/148‑1 (MQ=255)
                                             gCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTAtt                                                                                                      >  6:304457/1‑147 (MQ=255)
                                                  gtgCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTTacaca                                                                                                >  8:237899/1‑148 (MQ=255)
                                                  gtgCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGacaca                                                                                                >  5:41298/1‑148 (MQ=255)
                                                       ttGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACaa                                                                                             >  1:3547/1‑146 (MQ=255)
                                                       ttGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACaa                                                                                             >  4:40005/1‑146 (MQ=255)
                                                        tGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGct                                                                                                                               >  7:20435/1‑111 (MQ=255)
                                                        tGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGct                                                                                                                               <  8:20435/111‑1 (MQ=255)
                                                            caAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGtt                                                                                      >  3:269609/1‑148 (MQ=255)
                                                             aaggaaaggaTTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTg                                                                                     <  5:304457/148‑1 (MQ=255)
                                                             aaggaaaggaGTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTg                                                                                     <  8:70930/148‑1 (MQ=255)
                                                                 aaaggaTTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAgc                                                                                  >  5:45874/1‑146 (MQ=255)
                                                                         cATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATATTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGt                                                                            >  1:308251/1‑145 (MQ=255)
                                                                                 tCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTATTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGt                                                                 >  8:106037/1‑148 (MQ=255)
                                                                                    ttttCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTTATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGtttt                                                              <  8:304504/148‑1 (MQ=255)
                                                                                      ttcttcTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAg                                                            >  4:216569/1‑148 (MQ=255)
                                                                                           ctcCTGAAAGCGAGGTGGTCCATAGCGTAGGCTTACTGATAACTGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGGTGAGGAACAGTGAGATGATCGTTTTAGTaa                                                         <  6:267218/146‑1 (MQ=255)
                                                                                              cTGTACGCGAGGTTTTTCATAGCGTATGCTTACTTTTAACGGAGTGCAATCTTGCAATCCATTATTACCCTCTCTTAAGCATCCCGTGCTATGTTATTGACACACAATAGCGGTGAGGTACAGTGAGATGATCGTTTTAGTAACTgg                                                     <  1:69077/147‑1 (MQ=255)
                                                                                                gAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGtttt                                                              <  4:235405/136‑1 (MQ=255)
                                                                                                gAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGtttt                                                              >  3:235405/1‑136 (MQ=255)
                                                                                                 aaCGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAg                                                   <  3:40005/146‑1 (MQ=255)
                                                                                                  aCGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCaa                                                >  8:268607/1‑148 (MQ=255)
                                                                                                         gtgtTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTaa                                                         <  2:100375/132‑1 (MQ=255)
                                                                                                         gtgtTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTaa                                                         >  1:100375/1‑132 (MQ=255)
                                                                                                           gtTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGtt                                       >  5:209138/1‑148 (MQ=255)
                                                                                                             tCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTa                                                          >  7:88906/1‑127 (MQ=255)
                                                                                                             tCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTa                                                          <  8:88906/127‑1 (MQ=255)
                                                                                                             tCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGtttt                                     >  1:25013/1‑148 (MQ=255)
                                                                                                             tCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGtttt                                     >  7:222952/1‑148 (MQ=255)
                                                                                                              ccATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGtttt                                     <  4:269609/147‑1 (MQ=255)
                                                                                                                 tAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTg                                 <  7:268607/148‑1 (MQ=255)
                                                                                                                   gCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGaa                               >  6:39400/1‑148 (MQ=255)
                                                                                                                   gCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGa                                <  2:25013/147‑1 (MQ=255)
                                                                                                                             aCTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTAc                       <  3:216569/146‑1 (MQ=255)
                                                                                                                             aCTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACtc                     >  6:93027/1‑148 (MQ=255)
                                                                                                                                 aTAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGttt                                      <  4:41717/127‑1 (MQ=255)
                                                                                                                                 aTAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGttt                                      >  3:41717/1‑127 (MQ=255)
                                                                                                                                     cGGATTGCAATAGTGCAATCCAGTATTACCCGCTCTTAAGCATCCCCTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGtttt             <  6:45874/148‑1 (MQ=255)
                                                                                                                                      ggATTGCAAGATTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGa                                <  8:110748/128‑1 (MQ=255)
                                                                                                                                      ggATTGCAAGATTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGa                                >  7:110748/1‑128 (MQ=255)
                                                                                                                                           gCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTcaa       >  6:197118/1‑148 (MQ=255)
                                                                                                                                            cAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTcaa       <  2:3547/147‑1 (MQ=255)
                                                                                                                                                cTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGGTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAgg  >  5:113009/1‑148 (MQ=255)
                                                                                                                                                cTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTa                                                          <  5:255974/92‑1 (MQ=255)
                                                                                                                                                cTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTa                                                          >  6:255974/1‑92 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GATAGTGATGATTGGCAATTTGATCAAAATGGGGAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGG  >  NC_000913/1627296‑1627587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑