Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 210,634 A→G 5.3% K319R (AAA→AGA)  ldcC → lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913210,6340AG 5.3% 246.2 / 2.1 76K319R (AAA→AGA) ldcClysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base A (39/32);  new base G (2/2);  total (41/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTGGTGGGATCCCGCGCCGTGAATTTACTCGCGACAGCATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAAT  >  NC_000913/210491‑210781
                                                                                                                                               |                                                                                                                                                   
tCTTGGTGGGATCCCGCGCCGTGAATTTACTCGCGACAGCATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCaaa                                                                                                                                                    >  4:242222/1‑145 (MQ=255)
        ggATCCCGCGCCGTGAATTTACTCGCGACAGCATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGa                                                                                                                                         <  2:100885/148‑1 (MQ=255)
          aTCCCGCGCCGTGAATTTACTCGCGACAGCATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATg                                                                                                                                       >  4:40515/1‑148 (MQ=255)
                     tGAATTTACTCGCGACAGCATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGAtt                                                                                                                            >  3:195545/1‑148 (MQ=255)
                        aTTTACTCGCGACAGCATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCAc                                                                                                                         <  3:40515/148‑1 (MQ=255)
                          ttACTCGCGACAGCATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACtt                                                                                                                       >  3:7882/1‑148 (MQ=255)
                               cgcgACAGCATCGAAGAGAAAGTCGCTGCTACCACGCACGCACAATGGCCGGTTAATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTATACAACACCGACTGGATCAAACAGACGCTGGATGTCCAGTCGATTCACTTAGagt                                                                                                                  >  3:28240/1‑146 (MQ=255)
                               cgcgACAGCATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAtt                                                                                                                  >  7:198550/1‑148 (MQ=255)
                                 cgACAGCATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATAAAACAGACGCGGGATGTCCCGTCGATTCACTTAGATTCt                                                                                                                >  4:222819/1‑148 (MQ=255)
                                      gCATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAg                                                                                                                                                 >  6:44619/1‑110 (MQ=255)
                                      gCATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAg                                                                                                                                                 <  5:44619/110‑1 (MQ=255)
                                       cATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGcc                                                                                                             >  4:8118/1‑145 (MQ=255)
                                       cATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGcc                                                                                                             <  3:8118/145‑1 (MQ=255)
                                        aTCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTggg                                                                                                         >  6:65126/1‑148 (MQ=255)
                                          cGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCg                                                                                                                     <  4:154798/134‑1 (MQ=255)
                                          cGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCg                                                                                                                     >  3:154798/1‑134 (MQ=255)
                                          cGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGAGATCACCCACTCCACCTATGCTGGCTTGCTCTACAACACCTACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTg                                                                                                       >  6:8096/1‑148 (MQ=255)
                                            aaGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGcc                                                                                                     >  5:205694/1‑148 (MQ=255)
                                             agagAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt                                                                                                                   <  3:52938/133‑1 (MQ=255)
                                             agagAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGAGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt                                                                                                                   >  4:52938/1‑133 (MQ=255)
                                              gagaAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGt                                                                                                   <  3:254174/148‑1 (MQ=255)
                                              gagaAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGt                                                                                                   <  3:258488/148‑1 (MQ=255)
                                                  aaGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGt                                                                                                                                 >  1:36836/1‑114 (MQ=255)
                                                  aaGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTacac                                                                                               >  1:234259/1‑148 (MQ=255)
                                                  aaGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTAGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGt                                                                                                                                 <  2:36836/114‑1 (MQ=255)
                                                       gctgctACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAtt                                                                                          <  3:222819/148‑1 (MQ=255)
                                                        ctgctACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAttt                                                                                         >  2:6032/1‑148 (MQ=255)
                                                         tgctACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAttt                                                                                         >  5:69574/1‑147 (MQ=255)
                                                          gctACCACGCAAGCACAATGCCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAtttt                                                                                        >  2:125559/1‑147 (MQ=255)
                                                             aCCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAg                                                                                                                                                 >  5:130528/1‑87 (MQ=255)
                                                                  gCAAGCACAATGACCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATc                                                                               >  8:197720/1‑148 (MQ=255)
                                                                    aaGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTa                                                                             <  8:158225/148‑1 (MQ=255)
                                                                      gCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTAcc                                                                           <  3:3874/148‑1 (MQ=255)
                                                                       cacaATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCa                                                                          <  3:242222/148‑1 (MQ=255)
                                                                       cacaATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCa                                                                          <  8:198550/148‑1 (MQ=255)
                                                                        acaATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAg                                                                         <  6:205694/148‑1 (MQ=255)
                                                                           aTGGCCGGTTCATGCGGTGATAACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACAGGATCAAACAGACGCTGGATGTCACGTCGATTCACTTCGATTCTGCCAGGCTGCCGTACAACCATTTTCATCCGATCTACCAggg                                                                       >  8:168845/1‑147 (MQ=255)
                                                                                 ggTGCAAGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCACACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGt                                                                <  6:46428/148‑1 (MQ=255)
                                                                                    tCATGCGGGGATCAACAACTCCACCTATGATTGCTTGCTCTACAACACCGCCTGGATCAAACAGACGCGGGATGGCCCGGCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGt                                                             <  4:195545/148‑1 (MQ=255)
                                                                                     cATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGTTAAAAGTGGt                                                             <  8:125456/147‑1 (MQ=255)
                                                                                     cATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGt                                                             <  6:69574/147‑1 (MQ=255)
                                                                                     cATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGt                                                             <  7:197720/147‑1 (MQ=255)
                                                                                       ttcGGTGATCACCAACTCCACCTATTATGGCTTGCTCTACAACACCGAATGGATCAAACAGACGCTGTATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATg                                                          <  3:110858/146‑1 (MQ=255)
                                                                                        gCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTAt                                                           >  6:115291/1‑146 (MQ=255)
                                                                                                 aCCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAg                                                 <  5:65126/147‑1 (MQ=255)
                                                                                                   cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAtttt                                                                                        >  7:291171/1‑106 (MQ=255)
                                                                                                   cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCAtttt                                                                                        <  8:291171/106‑1 (MQ=255)
                                                                                                   cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCgt                                              >  7:59553/1‑148 (MQ=255)
                                                                                                        ccACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGc                                         >  6:292913/1‑148 (MQ=255)
                                                                                                         cACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGc                                         <  7:112098/147‑1 (MQ=255)
                                                                                                           ccTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCgg                                       >  8:96367/1‑147 (MQ=255)
                                                                                                            cTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTAcc                                                                           >  1:228085/1‑110 (MQ=255)
                                                                                                            cTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTAcc                                                                           <  2:228085/110‑1 (MQ=255)
                                                                                                              atgatgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGaa                                    >  8:167428/1‑147 (MQ=255)
                                                                                                                  tgGCTTGCTCTACTACACCTACTGGATCTAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACAGCCATTTTCATCCGATCTACCAGGGTTAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGATAAGTg                               <  5:138970/148‑1 (MQ=255)
                                                                                                                           ctACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa                       >  7:39289/1‑147 (MQ=255)
                                                                                                                           ctACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGGCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTaaa                                                                   <  7:262047/103‑1 (MQ=255)
                                                                                                                             acaacaCCGACTGGATCAGACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGa                                                                                 >  4:50836/1‑87 (MQ=255)
                                                                                                                             acaacaCCGACTGGATCAGACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGa                                                                                 <  3:50836/87‑1 (MQ=255)
                                                                                                                              caacaCCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGAGTTGCGGGAAAAGTGATCTTCGAAAct                   >  4:77308/1‑147 (MQ=255)
                                                                                                                               aacaCCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGCTCTTCGAAACg                   >  1:298325/1‑147 (MQ=255)
                                                                                                                               aacaCCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaaa                     <  3:118259/145‑1 (MQ=255)
                                                                                                                               aacaCCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaaa                     >  4:118259/1‑145 (MQ=255)
                                                                                                                                acacCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGc                  >  1:145656/1‑147 (MQ=255)
                                                                                                                                acacCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCa                 <  6:67071/148‑1 (MQ=255)
                                                                                                                                  acCGACTGGATCAGACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATc                                                                                    >  3:24297/1‑79 (MQ=255)
                                                                                                                                  acCGACTGGATCAGACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATc                                                                                    <  4:24297/79‑1 (MQ=255)
                                                                                                                                  acCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAAt               <  1:125559/148‑1 (MQ=255)
                                                                                                                                     gACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGa            <  2:234259/148‑1 (MQ=255)
                                                                                                                                       cTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc          >  8:235214/1‑148 (MQ=255)
                                                                                                                                          gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCca        >  6:250097/1‑147 (MQ=255)
                                                                                                                                           aTCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCAt                                                                                     <  7:36900/69‑1 (MQ=255)
                                                                                                                                           aTCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCAt                                                                                     >  8:36900/1‑69 (MQ=255)
                                                                                                                                            tCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACACAATCGACCcaca      >  3:58766/1‑147 (MQ=255)
                                                                                                                                             cAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTAcc                                                                           >  1:274638/1‑77 (MQ=255)
                                                                                                                                             cAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTAcc                                                                           <  2:274638/77‑1 (MQ=255)
                                                                                                                                             cAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaa     >  1:18472/1‑147 (MQ=255)
                                                                                                                                              aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa   <  7:96367/148‑1 (MQ=255)
                                                                                                                                               aaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAAt  >  2:11473/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TCTTGGTGGGATCCCGCGCCGTGAATTTACTCGCGACAGCATCGAAGAGAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAAT  >  NC_000913/210491‑210781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: