Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,366,090 A→G 5.0% S55G (AGT→GGT)  arnB → uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,366,0900AG 5.0% 257.3 / 2.2 79S55G (AGT→GGT) arnBuridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (38/37);  new base G (2/2);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACC  >  NC_000913/2365946‑2366237
                                                                                                                                                |                                                                                                                                                   
ttttCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTGAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGGCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGtt                                                                                                                                                  >  8:58582/1‑148 (MQ=255)
  ttCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTc                                                                                                                                                 <  2:6208/147‑1 (MQ=255)
            gCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCAccgccg                                                                                                                                      >  3:67860/1‑148 (MQ=255)
            gCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTAAGTTCAGCCAccgccg                                                                                                                                      >  3:225657/1‑148 (MQ=255)
             cAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTAGAGCAAGCTTATTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCGGTTCAGCCAccgccg                                                                                                                                      >  5:313520/1‑147 (MQ=255)
              aaTGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGTTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGa                                                                                                                                    <  4:8661/148‑1 (MQ=255)
                tGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAAt                                                                                                                                  >  8:62630/1‑148 (MQ=255)
                  ggCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATg                                                                                                                                 >  8:201514/1‑147 (MQ=255)
                   gCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCa                                                                                                                               <  2:304945/148‑1 (MQ=255)
                             aCTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCa                                                                                                                               <  4:262048/138‑1 (MQ=255)
                             aCTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCa                                                                                                                               >  3:262048/1‑138 (MQ=255)
                             aCTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTa                                                                                                                     <  7:219216/148‑1 (MQ=255)
                              tgatctacAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTa                                                                                                                     <  5:126904/140‑1 (MQ=255)
                                cGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGa                                                                                                                                    >  2:226131/1‑130 (MQ=255)
                                cGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGa                                                                                                                                    <  1:226131/130‑1 (MQ=255)
                                     cAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCg                                                                                                               <  1:26900/146‑1 (MQ=255)
                                         tAAAGAGGTTCTCGAATCCGGTTGGTTCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCAccgccg                                                                                                                                      <  1:211721/119‑1 (MQ=255)
                                         tAAAGAGGTTCTCGAATCCGGTTGGTTCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCAccgccg                                                                                                                                      >  2:211721/1‑119 (MQ=255)
                                          aaaGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGc                                                                                                                                >  4:192089/1‑124 (MQ=255)
                                          aaaGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGc                                                                                                                                <  3:192089/124‑1 (MQ=255)
                                          aaaGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGaaaa                                                                                                        >  3:295987/1‑148 (MQ=255)
                                           aaGAGGATCTCGAATCCGGTTGGATCACCACCGGTCCGAAGAATAAGGCGCTTGAGCAAGCTTTTTGCCAGTTGCCGGGAAATCAGCATGCCAACGCGGTCAGTTCAGCCAACGCCCGAATGCATATCACGATAATGGCGTTGAAAAt                                                                                                       >  1:102034/1‑148 (MQ=255)
                                               ggTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTgg                                                                                                    >  2:314344/1‑147 (MQ=255)
                                                 ttCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCaa                                                                                                 <  7:201514/148‑1 (MQ=255)
                                                 ttCTCGAATCAGGTTGGATCACAACCGGTCCGAAGAATCAGGCGATTGAGCAAGATTTTTGCCAGTTGACGGGAAATCATCATGCCATCGCGGTCAGTTCAGCCAACGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGc                                                                                                   >  7:200160/1‑146 (MQ=255)
                                                   ctcGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAgg                                                                                               >  6:153631/1‑148 (MQ=255)
                                                    tcGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTa                                                                                                                     <  8:219628/125‑1 (MQ=255)
                                                    tcGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTa                                                                                                                     >  7:219628/1‑125 (MQ=255)
                                                    tcGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAggg                                                                                              >  6:106585/1‑148 (MQ=255)
                                                    tcGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGAAGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCTCCGGAATGCATATCAAGCTAATGGAGTTGAAAATTGGCAAggt                                                                                              >  6:235670/1‑147 (MQ=255)
                                                     cGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGc                                                                                             >  4:274927/1‑148 (MQ=255)
                                                     cGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGc                                                                                             >  3:186880/1‑148 (MQ=255)
                                                      gAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCg                                                                                            <  8:119354/148‑1 (MQ=255)
                                                      gAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGGCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAgg                                                                                               <  4:279500/145‑1 (MQ=255)
                                                        aTCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGAt                                                                                          >  5:181545/1‑148 (MQ=255)
                                                           cGGTTTGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGTAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGa                                                                                        <  3:274927/147‑1 (MQ=255)
                                                                 gATCACATCCGGTCAGATGAATCAGGCGCTTGATCAAGCTTTTTGCCAGTTGACGGGTAATGAGGATGCCATCGCGGTCAGTTCAGCCATCGCCGGAATGCATATCACGCTAATGGCGTTGAAATTTGGCAAGGGCGATGAAGTGAtt                                                                                 <  2:102034/148‑1 (MQ=255)
                                                                 gATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGAtt                                                                                 <  4:139067/148‑1 (MQ=255)
                                                                 gATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGAtt                                                                                 <  5:243562/148‑1 (MQ=255)
                                                                  aTCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTa                                                                                <  7:294264/148‑1 (MQ=255)
                                                                    cacaACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCGGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACg                                                                              >  6:39099/1‑148 (MQ=255)
                                                                        aCCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCa                                                                                                                               >  3:209888/1‑95 (MQ=255)
                                                                        aCCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCa                                                                                                                               <  4:209888/95‑1 (MQ=255)
                                                                        aCCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCtt                                                                          >  1:112871/1‑148 (MQ=255)
                                                                         ccGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTc                                                                         <  4:67860/148‑1 (MQ=255)
                                                                         ccGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTc                                                                         <  8:287937/148‑1 (MQ=255)
                                                                          cGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTcc                                                                        >  7:110527/1‑148 (MQ=255)
                                                                           ggTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGACATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTccc                                                                       <  6:53368/148‑1 (MQ=255)
                                                                             tCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCGGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAATGGCGATGAAGTGATTACGCCTTCCCTg                                                                     <  6:313520/148‑1 (MQ=255)
                                                                             tCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTg                                                                     <  3:164207/148‑1 (MQ=255)
                                                                               cGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGa                                                                    <  2:112871/147‑1 (MQ=255)
                                                                                  agaaTCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTg                                                                <  4:225657/148‑1 (MQ=255)
                                                                                    aaTCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGCCGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTggg                                                              >  8:99454/1‑148 (MQ=255)
                                                                                       cAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGttt                                                           >  7:79386/1‑148 (MQ=255)
                                                                                          gcgcTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCaa                                                        >  8:64439/1‑148 (MQ=255)
                                                                                           cgcTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAAc                                                       <  7:62630/148‑1 (MQ=255)
                                                                                             cTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAAccc                                                     <  2:166602/148‑1 (MQ=255)
                                                                                                 aGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCaa                                                 >  1:7286/1‑148 (MQ=255)
                                                                                                 aGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCa                                                  <  4:186880/147‑1 (MQ=255)
                                                                                                  gCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAat                                                <  1:314344/148‑1 (MQ=255)
                                                                                                         ttttGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGAttt                                         >  6:28320/1‑148 (MQ=255)
                                                                                                         ttttGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGAttt                                         >  5:195230/1‑148 (MQ=255)
                                                                                                         ttttGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGAttt                                         >  7:63536/1‑148 (MQ=255)
                                                                                                              ccAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCttg                                    <  4:295987/148‑1 (MQ=255)
                                                                                                              ccAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCttg                                    <  2:7286/148‑1 (MQ=255)
                                                                                                                aGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACAGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCAttgtt                                  >  1:320325/1‑148 (MQ=255)
                                                                                                                   tGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTggg                               >  1:118493/1‑148 (MQ=255)
                                                                                                                     aCGGGAAATCAGCATGCCAGAGCGGTCAGATCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTg                             <  3:157804/148‑1 (MQ=255)
                                                                                                                       gggAAATCAGCATGCCATCGCGGTCGGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCa                           <  5:39099/148‑1 (MQ=255)
                                                                                                                              cAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCgg                    >  3:16174/1‑148 (MQ=255)
                                                                                                                               aGCATGCCATCGCGGTCAGTTCAGCCACCCCCGGAATGCATATCACGCTAATGGCGTTTAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTTACCTGGGTTTCAACCCTCAAGATGATTTCCTTGTTGGGTGCAACGCCgg                    >  8:298689/1‑147 (MQ=255)
                                                                                                                                gCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGt                   >  2:287670/1‑147 (MQ=255)
                                                                                                                                    gCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCttgt                                   >  7:7021/1‑127 (MQ=255)
                                                                                                                                    gCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCttgt                                   <  8:7021/127‑1 (MQ=255)
                                                                                                                                    gCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAAtgg              >  3:109254/1‑148 (MQ=255)
                                                                                                                                    gCCATCGCGGTCAGTTCAGCCACAGCCGGAATGCATATCACGCTAATGGCGTTGAAAATAGTCAAGGGCGATGAAGTGATTACGCATTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGACGGTAAtgg              >  3:169389/1‑148 (MQ=255)
                                                                                                                                     ccATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAAtggt             <  5:106585/148‑1 (MQ=255)
                                                                                                                                       aTCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAAtggtgg           <  5:153631/148‑1 (MQ=255)
                                                                                                                                           cgGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGt       <  8:79386/148‑1 (MQ=255)
                                                                                                                                                aGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAcc  <  7:64439/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TTTTCGCGACCAGCAATGGGCGTGGAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAGAATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACC  >  NC_000913/2365946‑2366237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑