Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,420,218 | G→A | 6.3% | R90C (CGT→TGT) | yfcF ← | glutathione S‑transferase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,420,218 | 0 | G | A | 6.3% | 205.2 / 3.1 | 64 | R90C (CGT→TGT) | yfcF | glutathione S‑transferase |
Reads supporting (aligned to +/- strand): ref base G (29/30); new base A (2/2); total (32/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 |
CCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAACCTTGCCACGTCGGCTGCAAATGTT > NC_000913/2420074‑2420355 | ccTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTc < 3:156886/148‑1 (MQ=255) tCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGt > 5:49242/1‑144 (MQ=255) tCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGt < 6:49242/144‑1 (MQ=255) cGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCCGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTcc > 1:216662/1‑146 (MQ=255) ggCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGgcg > 7:176250/1‑148 (MQ=255) gcgcTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGgcg > 6:51049/1‑147 (MQ=255) cgcTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGCCGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGgcgc > 1:234331/1‑147 (MQ=255) cTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCaaa > 2:26019/1‑148 (MQ=255) tttCGCCCCCACAAAGACAACATCCGTCGGACGCTCTTCGCTGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCg < 2:75939/147‑1 (MQ=255) ttCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGa < 1:26019/147‑1 (MQ=255) ttCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGa < 5:298512/147‑1 (MQ=255) aacaTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCa < 8:176250/148‑1 (MQ=255) acaTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCaa > 7:61404/1‑148 (MQ=255) caTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACATTCCCAGGTCGGTGGCGCAAATCGATCTTcc < 6:294162/134‑1 (MQ=255) caTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACATTCCCAGGTCGGTGGCGCAAATCGATCTTcc > 5:298848/1‑134 (MQ=255) caTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACATTCCCAGGTCGGTGGCGCAAATCGATCTTcc > 5:294162/1‑134 (MQ=255) caTCAGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACATTCCCAGGTCGGTGGCGCAAATCGATCTTcc < 6:298848/134‑1 (MQ=255) tCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGAt > 2:100674/1‑136 (MQ=255) tCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGAt < 1:100674/136‑1 (MQ=255) ccGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATgg > 7:158968/1‑147 (MQ=255) ggACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCTATGGCagaag < 2:223859/148‑1 (MQ=255) tGGGCAGCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTATAAATCAAGCGGATAAATAAGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCaaa < 5:235468/148‑1 (MQ=255) ggCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAat > 5:21076/1‑148 (MQ=255) cAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCtt > 4:98679/1‑101 (MQ=255) cAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCtt < 3:98679/101‑1 (MQ=255) cAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAatcatc > 4:137339/1‑147 (MQ=255) aGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGa > 5:272643/1‑148 (MQ=255) gATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGAt < 3:137339/148‑1 (MQ=255) aTCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGAtt > 6:136279/1‑148 (MQ=255) aTCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGAtt < 8:61404/148‑1 (MQ=255) gCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTg > 8:229960/1‑147 (MQ=255) gCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCAGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGt < 2:234331/148‑1 (MQ=255) gcAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGtaataa < 7:229960/148‑1 (MQ=255) cAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAAc < 5:136279/148‑1 (MQ=255) aGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACg < 8:158968/148‑1 (MQ=255) gCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGAGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTAGTAATAACgg > 4:301571/1‑148 (MQ=255) gAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAatcat > 4:43932/1‑123 (MQ=255) gAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAatcat < 3:43932/123‑1 (MQ=255) gAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGta > 3:237802/1‑132 (MQ=255) gAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGta < 4:237802/132‑1 (MQ=255) gAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGta > 1:314480/1‑132 (MQ=255) gAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGta < 2:314480/132‑1 (MQ=255) aaTCTGTCGTGCACGCGCACGATAATCTAAATCAAGCGGATAAAAACCTACCCAGCTCGTAGGCGCAAATAGATCATCCAGATACTCCGCAAAGGAAGAAGAAACACACAACTCAAAATCATCGATTTGTAATAACGGCACACgtctt > 8:257081/1‑144 (MQ=255) aTCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCgtg < 6:21076/148‑1 (MQ=255) tCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCgtgt > 3:125477/1‑148 (MQ=255) tCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCgtg > 3:39776/1‑147 (MQ=255) tCTGTCGTGCACGCGCACGAATTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGAATTGTAATAACGCCACACGGCgtg > 8:127497/1‑147 (MQ=255) tGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGta < 1:59688/127‑1 (MQ=255) tGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGta > 2:59688/1‑127 (MQ=255) tGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCt > 1:34883/1‑148 (MQ=255) tCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTg < 5:51049/147‑1 (MQ=255) cgcgCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGAt < 7:299780/70‑1 (MQ=255) cgcgCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGAt > 8:299780/1‑70 (MQ=255) cgcgCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAAc > 6:303412/1‑148 (MQ=255) cgcACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAACCt < 2:274274/148‑1 (MQ=255) cACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACg > 5:71382/1‑120 (MQ=255) cACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACg < 6:71382/120‑1 (MQ=255) ttCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAACCt > 1:47561/1‑139 (MQ=255) ttCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAACCt < 2:47561/139‑1 (MQ=255) tAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAACCTTGCCACGTCGGc < 2:34883/148‑1 (MQ=255) aaTCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAACCTTGCCACGTCGGc < 4:125477/146‑1 (MQ=255) gCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAACCTTGCCACGTCGGCTGCAAATg > 2:262716/1‑148 (MQ=255) cGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTGCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAACCTTGCCACGTCGGCTGCAAATGt < 5:215491/148‑1 (MQ=255) ggATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAACCTTGCCACGTCGGCTGCAAATGtt < 1:262716/148‑1 (MQ=255) | CCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAACCTTGCCACGTCGGCTGCAAATGTT > NC_000913/2420074‑2420355 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |