Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,436,760 C→T 5.4% L364L (TTG→TTA pdxB ← erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,436,7600CT 5.4% 249.7 / 2.9 74L364L (TTG→TTApdxBerythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (36/34);  new base T (2/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

TGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACAC  >  NC_000913/2436614‑2436906
                                                                                                                                                  |                                                                                                                                                  
tGCGCCCAGGATGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGc                                                                                                                                                    >  6:245565/1‑147 (MQ=255)
   gcCCAGGATGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAatg                                                                                                                                                <  5:245565/148‑1 (MQ=255)
   gcCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAatg                                                                                                                                                <  8:45427/148‑1 (MQ=255)
      cAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAatgatg                                                                                                                                             >  2:227176/1‑148 (MQ=255)
             gCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACt                                                                                                                                      <  8:123986/148‑1 (MQ=255)
              cAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCa                                                                                                                                                   <  5:299963/134‑1 (MQ=255)
              cAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCa                                                                                                                                                   >  6:299963/1‑134 (MQ=255)
                   ttCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGt                                                                                                                                 >  7:110392/1‑147 (MQ=255)
                    tCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCg                                                                                                                                  <  6:68232/145‑1 (MQ=255)
                    tCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCg                                                                                                                                  >  5:68232/1‑145 (MQ=255)
                       aGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGtcatca                                                                                                                            >  3:35931/1‑148 (MQ=255)
                         ccTTCAGACATGGTGGATTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATcaca                                                                                                                          <  5:89819/148‑1 (MQ=255)
                              aGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAAtt                                                                                                                      <  1:227176/147‑1 (MQ=255)
                              aGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATta                                                                                                                     <  3:224504/148‑1 (MQ=255)
                                    ggtggtTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCa                                                                                                                                        <  2:246524/123‑1 (MQ=255)
                                    ggtggtTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCa                                                                                                                                        >  1:246524/1‑123 (MQ=255)
                                        gtTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACagag                                                                                                            <  4:35931/147‑1 (MQ=255)
                                              tCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAACCCCAGTTTACACAGTAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCa                                                                                                     >  6:222960/1‑148 (MQ=255)
                                              tCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGAcc                                                                                                      <  6:125156/147‑1 (MQ=255)
                                              tCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGAcc                                                                                                      >  5:125156/1‑147 (MQ=255)
                                               ccAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCAt                                                                                                    >  5:289197/1‑148 (MQ=255)
                                               ccAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCAt                                                                                                    >  2:314848/1‑148 (MQ=255)
                                               ccAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCAt                                                                                                    >  2:272089/1‑148 (MQ=255)
                                                 aGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTc                                                                                                  <  8:110392/148‑1 (MQ=255)
                                                                    gCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGTAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAg                                                                                      <  1:46560/141‑1 (MQ=255)
                                                                    gCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGTAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAg                                                                                      >  2:46560/1‑141 (MQ=255)
                                                                    gCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGttt                                                                               >  5:152898/1‑148 (MQ=255)
                                                                     ccAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGTAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGtttt                                                                              <  5:222960/148‑1 (MQ=255)
                                                                     ccAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGttt                                                                               <  4:214640/147‑1 (MQ=255)
                                                                       aaTGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTg                                                                            <  6:263093/148‑1 (MQ=255)
                                                                        aTGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAACCCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTgc                                                                           >  8:269621/1‑148 (MQ=255)
                                                                         tGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTgcg                                                                          >  1:95198/1‑148 (MQ=255)
                                                                          gTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACag                                                                                                              <  7:106603/111‑1 (MQ=255)
                                                                          gTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACag                                                                                                              >  8:106603/1‑111 (MQ=255)
                                                                          gTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTgcg                                                                          >  2:40795/1‑147 (MQ=255)
                                                                           ttACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTgcg                                                                          >  4:121621/1‑146 (MQ=255)
                                                                            tACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAg                                                                       >  8:143008/1‑148 (MQ=255)
                                                                                gcagAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGc                                                                                                                                            <  6:15732/75‑1 (MQ=255)
                                                                                gcagAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGc                                                                                                                                            >  5:15732/1‑75 (MQ=255)
                                                                                    agagCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTCAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGa                                                               <  3:121621/148‑1 (MQ=255)
                                                                                    agagCATGAAGAAGAGATTAACGTGCCGGAGGATGAACGGCGTTAACACCCAGTTTACTCAGCAATGATGCCGCACTGGCGTCAACACAATTTCCATAGCGAGATGACCCTTGACGGCGCTTAAGATAGTTTTTGCGCAGTTGATCg                                                                >  4:239392/1‑147 (MQ=255)
                                                                                     gagCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGa                                                               <  1:40795/147‑1 (MQ=255)
                                                                                            aagaagAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCATTTTATCGAACTCAccc                                                       <  6:152898/148‑1 (MQ=255)
                                                                                                agagATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACagag                                                                                                            <  5:143310/91‑1 (MQ=255)
                                                                                                agagATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACagag                                                                                                            >  6:143310/1‑91 (MQ=255)
                                                                                                  agaTTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTAt                                                                  >  4:84581/1‑131 (MQ=255)
                                                                                                  agaTTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTAt                                                                  <  3:84581/131‑1 (MQ=255)
                                                                                                     ttAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCg                                              >  5:11719/1‑148 (MQ=255)
                                                                                                      tAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCgg                                             >  5:2587/1‑148 (MQ=255)
                                                                                                      tAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCgg                                             >  5:6947/1‑148 (MQ=255)
                                                                                                      tAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCgg                                             >  3:216128/1‑148 (MQ=255)
                                                                                                             ccGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACtt                                       >  3:241922/1‑147 (MQ=255)
                                                                                                             ccGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACtt                                       <  1:279600/147‑1 (MQ=255)
                                                                                                             ccGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACtt                                       <  6:6947/147‑1 (MQ=255)
                                                                                                             ccGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACtt                                       <  6:2587/147‑1 (MQ=255)
                                                                                                               ggATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTAc                                    >  1:183002/1‑148 (MQ=255)
                                                                                                               ggATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTAc                                    <  2:95198/148‑1 (MQ=255)
                                                                                                                 atgatgAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACg                                   >  3:138286/1‑147 (MQ=255)
                                                                                                                        aCGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTg                           <  1:314848/148‑1 (MQ=255)
                                                                                                                         cGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGc                          >  6:181418/1‑148 (MQ=255)
                                                                                                                                  aaCCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATcgcg                 <  4:138286/148‑1 (MQ=255)
                                                                                                                                  aaCCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATcgcg                 >  8:256206/1‑148 (MQ=255)
                                                                                                                                    cccAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCgcggc               >  1:220301/1‑148 (MQ=255)
                                                                                                                                        gTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGcac           <  7:269621/148‑1 (MQ=255)
                                                                                                                                        gTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGcac           <  6:11719/148‑1 (MQ=255)
                                                                                                                                          ttACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATc                                                 <  4:55023/108‑1 (MQ=255)
                                                                                                                                          ttACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATc                                                 >  3:55023/1‑108 (MQ=255)
                                                                                                                                           tACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCAcat         <  7:256206/147‑1 (MQ=255)
                                                                                                                                            acacAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCAcatc        <  4:216128/147‑1 (MQ=255)
                                                                                                                                             cacaGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCAcatca       >  8:248999/1‑147 (MQ=255)
                                                                                                                                              acaGCAATGATGCCGCACTGTCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATa     <  1:272089/148‑1 (MQ=255)
                                                                                                                                              acaGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATa     <  7:143008/148‑1 (MQ=255)
                                                                                                                                               caGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATac    >  8:149366/1‑148 (MQ=255)
                                                                                                                                                 gCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATacac  >  5:75218/1‑148 (MQ=255)
                                                                                                                                                 gCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATacac  >  8:150808/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACAC  >  NC_000913/2436614‑2436906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: