Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,837,236 G→A 5.5% H64Y (CAC→TAC)  hypF ← carbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,837,2360GA 5.5% 242.6 / 2.7 73H64Y (CAC→TAC) hypFcarbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base G (35/34);  new base A (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGGGCAAGTAGCGGCATCGGGAACAATTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGC  >  NC_000913/2837094‑2837382
                                                                                                                                              |                                                                                                                                                  
gCAGGGCAAGTAGCGGCATCGGGAACAATTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCCCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGa                                                                                                                                                >  5:284071/1‑147 (MQ=255)
   gggCAAGTAGCGGCAGCGGGAACAATTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCGCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACa                                                                                                                                            <  3:187902/148‑1 (MQ=255)
     gCAAGTAGCGGCATCGGGAACAATTTGCGGATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAAt                                                                                                                                          >  1:249099/1‑148 (MQ=255)
         gTAGCGGCATCGGGAACAATTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGCACTCGGTGGGCAGTTGTGCCCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGaa                                                                                                                                      >  5:190594/1‑148 (MQ=255)
                cATCGGGAACAATTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGaaa                                                                                                                               >  2:162749/1‑148 (MQ=255)
                     ggAACAATTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGttt                                                                                                                          <  2:18147/148‑1 (MQ=255)
                      gAACAATTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTc                                                                                                                         <  5:107075/148‑1 (MQ=255)
                      gAACAATTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTc                                                                                                                         >  1:279842/1‑148 (MQ=255)
                       aaCAATTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTc                                                                                                                         >  5:145628/1‑147 (MQ=255)
                       aaCAATTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTc                                                                                                                         <  6:145628/147‑1 (MQ=255)
                            tttGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTTTAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTc                                                                                                                   >  4:157762/1‑148 (MQ=255)
                            tttGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTc                                                                                                                   >  3:94493/1‑148 (MQ=255)
                              tGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCtt                                                                                                                 >  1:188286/1‑148 (MQ=255)
                                cGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTcc                                                                                                               <  2:188286/148‑1 (MQ=255)
                                 gTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTccc                                                                                                              >  1:98908/1‑148 (MQ=255)
                                    ttCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGc                                                                                                            <  2:98908/147‑1 (MQ=255)
                                    ttCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGc                                                                                                            <  8:171563/147‑1 (MQ=255)
                                       aTGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGcc                                                                                                        <  1:55056/148‑1 (MQ=255)
                                          gTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGa                                                                                                     <  4:289140/148‑1 (MQ=255)
                                                ccggtgCTCTGGGGTATAGTGAACGCGGTGGGCAGTAGTGACCTGATAATGGGCTCTCGCTCGACTCTATCAATACGCGCCAGCGGCGGGCAGTGCTGAGACTATGGAACACGAACCGTATGCGGGGCTTCCCGCTGCCGGACTTCTa                                                                                               <  4:197831/145‑1 (MQ=255)
                                                  tgtgCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACg                                                                                             >  5:192140/1‑148 (MQ=255)
                                                        ctGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCg                                                                                       <  1:214319/148‑1 (MQ=255)
                                                          ggCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGcc                                                                                     >  3:140733/1‑148 (MQ=255)
                                                          ggCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGcc                                                                                     >  8:177403/1‑148 (MQ=255)
                                                          ggCGTATAGGGACCGCGGTGGGCAGGTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGcc                                                                                     <  5:44139/148‑1 (MQ=255)
                                                               ataGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTACTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCAt                                                                                >  3:287079/1‑148 (MQ=255)
                                                               ataGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTACTGATACAATTGAACAAGAAACGTTTCCGGGTCTTACCGCAGCCGGACTTCTACGCCATCGCCGTCAt                                                                                >  3:282559/1‑148 (MQ=255)
                                                                taGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCAtt                                                                               <  1:162749/148‑1 (MQ=255)
                                                                         cGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATc                                                                      >  5:44867/1‑148 (MQ=255)
                                                                             gggCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCAtt                                                                               >  7:293046/1‑135 (MQ=255)
                                                                             gggCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCAtt                                                                               <  8:293046/135‑1 (MQ=255)
                                                                             gggCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCg                                                                  >  7:151472/1‑148 (MQ=255)
                                                                                cAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGACCAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTg                                                                >  1:252159/1‑147 (MQ=255)
                                                                                    tgtgACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGcc                                                                   <  2:221387/140‑1 (MQ=255)
                                                                                    tgtgACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGcc                                                                   >  1:221387/1‑140 (MQ=255)
                                                                                    tgtgACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGAt                                                           >  4:96172/1‑148 (MQ=255)
                                                                                    tgtgACCAGATAAACGGCTCACGCTCGACGCTATCAATAAGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAAAGTTTCAGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCAGTCATTACAGACATAGCCGTGTAGAt                                                           >  8:259819/1‑148 (MQ=255)
                                                                                      tgACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGAtt                                                          <  6:192140/147‑1 (MQ=255)
                                                                                        aCCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCCTCGCCGTCATTACAGACATCGCCGTGAAGAttaa                                                        <  2:249099/147‑3 (MQ=255)
                                                                                           aGATAAACGGCTCACGCTCGACGCGATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCTCCGTCATTACAGACATCGCCGTGAAGATTTAATTg                                                    <  2:211596/148‑1 (MQ=255)
                                                                                              tAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCtg                                                 >  4:112607/1‑148 (MQ=255)
                                                                                              tAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCtg                                                 <  3:112607/148‑1 (MQ=255)
                                                                                                 aCGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGTCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGc                                              >  8:305239/1‑148 (MQ=255)
                                                                                                         cGCTCGACGCTATCAATACGCGCCAGCGGCGTGCAGTGCTGATACAATGTAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCGGcc                                      <  6:284071/148‑1 (MQ=255)
                                                                                                         cGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGcc                                      <  2:2178/148‑1 (MQ=255)
                                                                                                           cTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAg                                    <  7:177403/148‑1 (MQ=255)
                                                                                                             cGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGa                                   >  2:20200/1‑147 (MQ=255)
                                                                                                             cGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGGCATTACAGACCTCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAgc                                   >  5:248051/1‑146 (MQ=255)
                                                                                                               aCGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACaa                                <  3:96172/148‑1 (MQ=255)
                                                                                                                cGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTAAGCCAGCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACaaa                               <  6:190594/148‑1 (MQ=255)
                                                                                                                   tATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACg                             >  2:14815/1‑147 (MQ=255)
                                                                                                                    aTCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGa                           >  6:79055/1‑148 (MQ=255)
                                                                                                                     tCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACgg                            <  2:28772/146‑1 (MQ=255)
                                                                                                                      cAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGAc                          >  7:187646/1‑147 (MQ=255)
                                                                                                                       aaTACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGc                                       >  6:298494/1‑133 (MQ=255)
                                                                                                                       aaTACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGc                                       <  5:298494/133‑1 (MQ=255)
                                                                                                                          aCGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGTGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTa                                                        >  6:75624/1‑113 (MQ=255)
                                                                                                                          aCGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTa                                                        <  5:75624/113‑1 (MQ=255)
                                                                                                                           cgcgCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGc                                              >  8:132672/1‑122 (MQ=255)
                                                                                                                           cgcgCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGc                                              <  7:132672/122‑1 (MQ=255)
                                                                                                                             cgcCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCg                  <  8:187646/148‑1 (MQ=255)
                                                                                                                              gcCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGa                 >  4:223121/1‑148 (MQ=255)
                                                                                                                                cAGCGGCGGGCAGTACTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACg               <  4:287079/148‑1 (MQ=255)
                                                                                                                                cAGCGGCGGGCAGTACTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACg               <  4:282559/148‑1 (MQ=255)
                                                                                                                                        ggCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCAc       <  5:79055/148‑1 (MQ=255)
                                                                                                                                          cAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACtt     <  3:157762/148‑1 (MQ=255)
                                                                                                                                          cAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACtt     <  6:44867/148‑1 (MQ=255)
                                                                                                                                          cAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACtt     >  4:58554/1‑148 (MQ=255)
                                                                                                                                            gTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCa                                            >  6:150318/1‑107 (MQ=255)
                                                                                                                                            gTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCa                                            <  5:150318/107‑1 (MQ=255)
                                                                                                                                             tGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGc                                                   <  4:293601/99‑1 (MQ=255)
                                                                                                                                             tGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGc                                                   >  3:293601/1‑99 (MQ=255)
                                                                                                                                             tGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGc  >  3:140671/1‑148 (MQ=255)
                                                                                                                                              gCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCt                                                  <  6:105378/99‑1 (MQ=255)
                                                                                                                                              gCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCt                                                  >  5:105378/1‑99 (MQ=255)
                                                                                                                                              gCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGc  >  2:200616/1‑147 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
GCAGGGCAAGTAGCGGCATCGGGAACAATTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTCACGCTCGACGCTATCAATACGCGCCAGCGGCGGGCAGTGCTGATACAATTGAACAAGAAACGTTTCCGGGTCTTCCCGCAGCCGGACTTCTACGCCATCGCCGTCATTACAGACATCGCCGTGAAGATTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGC  >  NC_000913/2837094‑2837382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑