Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1979486 =0 (0.000)35 (0.950) 27/236 0.5 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 19794890 (0.000)49 (1.320) 31/236 0.3 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region

AGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAAT  >  NC_000913/1979339‑1979634
                                                                                                                                                   |                                                                                                                                                    
aaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccc                                                                                                                                                      >  1:118675‑M1/148‑148 (MQ=255)
aaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCt                                                                                                                                                     >  2:176142‑M1/148‑149 (MQ=255)
       gggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTaaa                                                                                                                                              >  6:162341‑M1/141‑149 (MQ=255)
         aaaTGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggt                                                                                                                                            <  1:185010‑M1/149‑8 (MQ=255)
         aaaTGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggt                                                                                                                                            >  2:185010‑M1/1‑142 (MQ=255)
            aacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAt                                                                                                                                                  <  4:91950‑M1/5‑1 (MQ=255)
            aacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAt                                                                                                                                                  >  3:91950‑M1/136‑140 (MQ=255)
              cgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCt                                                                                                                                                     >  7:120420‑M1/134‑135 (MQ=255)
              cgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCt                                                                                                                                                     <  8:120420‑M1/2‑1 (MQ=255)
               gccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACaaag                                                                                                                                       <  7:136112‑M1/16‑1 (MQ=255)
                  aGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaag                                                                                                                                   >  5:175982‑M1/1‑133 (MQ=255)
                              aaTGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatga                                                                                                                       <  1:66005‑M1/149‑29 (MQ=255)
                                  cggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACttaa                                                                                                                   >  8:41233‑M1/114‑149 (MQ=255)
                                   gATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgaga                                                                                                                     >  8:110711‑M1/1‑116 (MQ=255)
                                   gATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgaga                                                                                                                     <  7:110711‑M1/146‑31 (MQ=255)
                                    gtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACttaatg                                                                                                                 <  3:90054‑M1/38‑1 (MQ=255)
                                              gctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAAt                                                                                                       >  7:159393‑M1/102‑149 (MQ=255)
                                                tgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACaaagaaag                                                                                                                                   <  7:61696‑M1/20‑1 (MQ=255)
                                                tgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACaaagaaag                                                                                                                                   >  8:61696‑M1/100‑119 (MQ=255)
                                                 tCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctg                                                                                                     >  5:29618‑M1/1‑102 (MQ=255)
                                                 tCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaac                                                                                                                                      >  1:24003‑M1/1‑102 (MQ=255)
                                                 tCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaac                                                                                                                                      <  2:24003‑M1/115‑14 (MQ=255)
                                                   ttcaatgcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGtttt                                                                                                  >  4:90054‑M1/97‑149 (MQ=255)
                                                     caaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTa                                                                                                 >  8:136112‑M1/95‑148 (MQ=255)
                                                      aaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttcccagcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTAc                                                                                                <  1:117920‑M1/55‑1 (MQ=255)
                                                           tCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgata                                                                                                                          >  2:135801‑M1/1‑92 (MQ=255)
                                                           tCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgata                                                                                                                          <  1:135801‑M1/117‑26 (MQ=255)
                                                                     taatCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccct                                                                                                                              >  6:104636‑M1/1‑82 (MQ=255)
                                                                     taatCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccct                                                                                                                              <  5:104636‑M1/103‑22 (MQ=255)
                                                                     gaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACatat                                                                                            >  3:82150‑M1/79‑137 (MQ=255)
                                                                     gaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACatat                                                                                            <  4:82150‑M1/59‑1 (MQ=255)
                                                                          atcTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatga                                                                           <  6:29618‑M1/149‑73 (MQ=255)
                                                                           atcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAAt                                                                          <  4:140228‑M1/77‑1 (MQ=255)
                                                                               cTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcat                                                                      >  7:73719‑M1/1‑72 (MQ=255)
                                                                                tataAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctgtagccatagaacagg                                                                                      >  8:56026‑M1/1‑71 (MQ=255)
                                                                                tataAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagg                                                                                      <  7:56026‑M1/132‑62 (MQ=255)
                                                                                 ataaataaTCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatct                                                                                                      <  2:98843‑M1/115‑46 (MQ=255)
                                                                                 ataaataaTCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatct                                                                                                      >  1:98843‑M1/1‑70 (MQ=255)
                                                                                  taaataaTCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaa                                                                                                                                    >  5:114900‑M1/1‑69 (MQ=255)
                                                                                  taaataaTCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaa                                                                                                                                    <  6:114900‑M1/84‑16 (MQ=255)
                                                                                   aaataaTCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaaca                                                                                        <  1:192058‑M1/127‑60 (MQ=255)
                                                                                   aaataaTCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaaca                                                                                        >  2:192058‑M1/1‑68 (MQ=255)
                                                                                     ataatCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaactt                                                                <  8:73719‑M1/149‑84 (MQ=255)
                                                                                     ataatCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagcca                                                                                               >  1:201282‑M1/1‑66 (MQ=255)
                                                                                     ataatCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagcca                                                                                               <  2:201282‑M1/118‑53 (MQ=255)
                                                                                        atCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaac                                                                                                                                      <  5:21900‑M1/76‑14 (MQ=255)
                                                                                        atCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaac                                                                                                                                      >  6:21900‑M1/1‑63 (MQ=255)
                                                                                          aagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAAt                                                                 >  3:137037‑M1/58‑143 (MQ=255)
                                                                                          aagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAAt                                                                 <  4:137037‑M1/86‑1 (MQ=255)
                                                                                             tttCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatctttgtcatctggagccatagaacacggttcatcaagagtcatcaacttac                                                              >  3:41644‑M1/1‑58 (MQ=255)
                                                                                                  tGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaa                                                                   <  1:109329‑M1/133‑81 (MQ=255)
                                                                                                  tGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaa                                                                   >  2:109329‑M1/1‑53 (MQ=255)
                                                                                                           agtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCtt                                                <  4:128744‑M1/103‑1 (MQ=255)
                                                                                                           agtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCtt                                                >  3:128744‑M1/41‑143 (MQ=255)
                                                                                                             tcagccgagaagaatttccccgcttattcgcaccttccCTAAt                                                                                                                                                  >  5:105052‑M1/39‑43 (MQ=255)
                                                                                                             tcagccgagaagaatttccccgcttattcgcaccttccCTAAt                                                                                                                                                  <  6:105052‑M1/5‑1 (MQ=255)
                                                                                                                   tATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaag                                  >  2:21544‑M1/1‑36 (MQ=255)
                                                                                                                         aatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATtat                            >  3:140228‑M1/27‑149 (MQ=255)
                                                                                                                            ttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTatat                          >  2:117920‑M1/24‑148 (MQ=255)
                                                                                                                               gACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagg                                                                                      >  7:156291‑M1/1‑24 (MQ=255)
                                                                                                                               gACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagg                                                                                      <  8:156291‑M1/85‑62 (MQ=255)
                                                                                                                                 cgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACat                                                                                              <  1:135239‑M1/57‑1 (MQ=255)
                                                                                                                                 cgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACat                                                                                              >  2:135239‑M1/19‑75 (MQ=255)
                                                                                                                                  gcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGgaga                                                                   <  5:107177‑M1/84‑1 (MQ=255)
                                                                                                                                  gcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGgaga                                                                   >  6:107177‑M1/18‑101 (MQ=255)
                                                                                                                                          gcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGtga           <  4:83862‑M1/140‑1 (MQ=255)
                                                                                                                                           caccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGtgat          >  8:166729‑M1/9‑149 (MQ=255)
                                                                                                                                                 ccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCAc                                                                                   >  8:140330‑M1/3‑70 (MQ=255)
                                                                                                                                                 ccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCAc                                                                                   <  7:140330‑M1/68‑1 (MQ=255)
                                                                                                                                                  aCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgcc                                                       <  4:53605‑M1/97‑93 (MQ=255)
                                                                                                                                                  aCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgcc                                                       >  3:53605‑M1/1‑5 (MQ=255)
                                                                                                                                                   cTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtc  <  1:92572‑M1/149‑146 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAAT  >  NC_000913/1979339‑1979634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.