| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,596,878 | 1 | . | G | 22.2% | 66.5 / 25.3 | 27 | intergenic (‑141/+27) | ypfJ/purC | putative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
| Reads supporting (aligned to +/- strand): ref base . (10/11); new base G (3/3); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTT > NC_000913/2596745‑2597007 | tCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAgatgat > 1:73640/1‑149 (MQ=255) aacaaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAgatgat < 2:73640/147‑1 (MQ=255) aacaaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGatgatg > 4:114053/1‑148 (MQ=255) caaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAAc < 3:114053/149‑1 (MQ=255) agagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAgat < 5:141477/106‑1 (MQ=255) agagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAgat > 6:141477/1‑106 (MQ=255) gCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGAt > 6:159215/1‑149 (MQ=255) ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAg > 6:50505/1‑149 (MQ=255) aTGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGCGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATc > 6:150767/1‑145 (MQ=255) aTGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATc < 5:150767/145‑1 (MQ=255) gTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACg < 3:73254/149‑1 (MQ=255) cAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGt < 4:111778/148‑1 (MQ=255) atatatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCAt < 8:21439/103‑1 (MQ=255) atatatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCAt > 7:21439/1‑103 (MQ=255) atatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCCCCGAGGCTCTGGCGGAAACGGGCTTTGTCCa > 8:65640/1‑149 (MQ=255) ggTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGt < 5:159215/149‑1 (MQ=255) gTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGtt < 5:50505/149‑1 (MQ=255) tCTTGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTg < 5:22637/111‑1 (MQ=255) tCTTGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTg > 6:22637/1‑111 (MQ=255) ttGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTg < 2:178598/52‑1 (MQ=21) ttGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTg > 1:178598/1‑52 (MQ=21) gCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCt < 7:142888/84‑1 (MQ=39) gCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCt > 8:142888/1‑84 (MQ=39) ccAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCttt > 4:12884/1‑116 (MQ=255) ccAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCttt < 3:12884/116‑1 (MQ=255) cT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGctct < 5:29655/96‑1 (MQ=255) cT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGctct > 6:29655/1‑96 (MQ=255) | TCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTT > NC_000913/2596745‑2597007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |