| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,525,439 | G→A | 5.4% | intergenic (+16/+150) | mrcA → / ← nudE | penicillin‑binding protein 1a, murein transglycosylase and transpeptidase/adenosine nucleotide hydrolase; Ap3A/Ap2A/ADP‑ribose/NADH hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,525,439 | 0 | G | A | 5.4% | 297.9 / 4.5 | 93 | intergenic (+16/+150) | mrcA/nudE | penicillin‑binding protein 1a, murein transglycosylase and transpeptidase/adenosine nucleotide hydrolase; Ap3A/Ap2A/ADP‑ribose/NADH hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (45/43); new base A (3/2); total (48/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01 | |||||||||||
CAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCAC > NC_000913/3525298‑3525584 | cAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCtt > 4:309163/1‑149 (MQ=255) cTAATGGGGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACACCAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGtt < 5:257962/149‑1 (MQ=255) tAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGttt > 1:11714/1‑149 (MQ=255) aaTGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTg > 7:220354/1‑149 (MQ=255) ttggtggCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGttt < 6:120860/146‑1 (MQ=255) aTGTTGGCAACAGCCGCGAAGATTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGTTGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAACAGGCGCCGAAGCGCCtttttaatca < 6:75188/147‑7 (MQ=255) tggtggCAACAGCCGCGAAGAGAATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGCCCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCt > 8:70631/1‑149 (MQ=255) gCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACa > 4:286447/1‑148 (MQ=255) aaCAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAg > 8:192659/1‑149 (MQ=255) aCAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGtt < 5:339515/137‑1 (MQ=255) aCAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGtt > 6:339515/1‑137 (MQ=255) cAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTg > 2:314262/1‑149 (MQ=255) gCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCa < 8:149027/149‑1 (MQ=255) gctaaGAGTATTTCATCTAAGGTACGCATCCGACACAACAGGAAGTGCACGAGGGGGGAACGACCTTTATCGATAATGGCGAGGCACAGTAATTGTTCTGATTAATAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACtt < 1:307754/146‑1 (MQ=255) gAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGt < 6:332195/149‑1 (MQ=255) gAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGt < 3:311417/149‑1 (MQ=255) aaGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGTCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGtt < 4:186757/149‑1 (MQ=255) gagTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTa > 5:125554/1‑149 (MQ=255) gagTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTa < 3:309919/149‑1 (MQ=255) gTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATg > 8:56235/1‑149 (MQ=255) tttCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCg > 4:295636/1‑149 (MQ=255) tttCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCg > 8:348227/1‑149 (MQ=255) tttCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCg > 3:170343/1‑149 (MQ=255) tCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATAc > 7:174568/1‑149 (MQ=255) gAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTGCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACgg < 5:126307/149‑1 (MQ=255) aaGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGc < 3:230613/149‑1 (MQ=255) aGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGc < 6:125554/148‑1 (MQ=255) ggTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGt < 8:49112/149‑1 (MQ=255) tACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTg > 7:150125/1‑148 (MQ=255) cGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAAc > 4:137317/1‑149 (MQ=255) aacaGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGCCAAATTGCACTTGTTTATGCCGGATACGGCGAGAACGCGTTATCCgg > 4:235449/1‑148 (MQ=255) aacaGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGa < 8:257251/125‑1 (MQ=255) aacaGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGa > 7:257251/1‑125 (MQ=255) aacaGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGc > 6:293868/1‑149 (MQ=255) aacaGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGc > 1:56933/1‑149 (MQ=255) aacaGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGc > 7:342846/1‑149 (MQ=255) aacaGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGc < 3:286447/149‑1 (MQ=255) aacaGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGc > 8:44525/1‑149 (MQ=255) acaGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGc > 2:323842/1‑148 (MQ=255) acaGGCAGTGCACGAGGTCGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGc > 6:16136/1‑148 (MQ=255) aGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTaa < 3:7309/149‑1 (MQ=255) ggCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAAc < 1:112507/149‑1 (MQ=255) gCAGTGCACGAGGTCGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACa < 1:171293/149‑1 (MQ=255) gCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATc < 2:254311/149‑1 (MQ=255) gAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAACATCGTg > 2:309623/1‑148 (MQ=255) gTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCATGAACGCGTTATCCGGCTAACAAAATCGTGaaaa > 8:119760/1‑149 (MQ=255) gggAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACt > 4:290187/1‑149 (MQ=255) ggAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATACGGCTAACAAAATCGTGAAAACt > 8:345226/1‑148 (MQ=255) aCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAAt < 5:293868/149‑1 (MQ=255) aCCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATaaa < 7:192659/149‑1 (MQ=255) ccATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAAt > 4:47445/1‑149 (MQ=255) aTTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTg < 2:56933/149‑1 (MQ=255) aTCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCa < 1:40256/148‑1 (MQ=255) aTAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTa > 5:126315/1‑82 (MQ=255) aTAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTa < 6:126315/82‑1 (MQ=255) aTAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAAc > 3:107640/1‑149 (MQ=255) tAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAAcc > 7:293377/1‑149 (MQ=255) tAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAAcc > 2:216541/1‑149 (MQ=255) aTGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAAcccc < 4:170343/149‑1 (MQ=255) aTGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAAccc < 3:290187/148‑1 (MQ=255) tGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCa > 5:167964/1‑149 (MQ=255) tGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCa > 2:67951/1‑149 (MQ=255) gCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATa < 7:348227/149‑1 (MQ=255) gCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATa < 3:269439/149‑1 (MQ=255) gCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCt > 3:33916/1‑149 (MQ=255) acaGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGa > 3:121164/1‑149 (MQ=255) aTTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACa < 4:33916/149‑1 (MQ=255) gTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACAtt < 8:342846/148‑1 (MQ=255) gTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACAtt < 4:121164/148‑1 (MQ=255) ttCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATtgt < 3:295636/149‑1 (MQ=255) ttCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATtgt < 7:44525/149‑1 (MQ=255) ttCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATtg < 8:150125/148‑1 (MQ=255) ttCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTATGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATtgt < 5:294667/149‑1 (MQ=255) cTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTATCAAAATCGTGAAAACTCAATAAATTGCGGAAACCCCATAAGCCTGATAAACATtgtg > 3:16453/1‑148 (MQ=255) actgaaaaGGCGCCGAAGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCAt > 5:274380/5‑148 (MQ=255) aTTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGc < 8:174568/146‑1 (MQ=255) aTTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCAt > 2:268255/1‑148 (MQ=255) aTTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATc < 7:70631/149‑1 (MQ=255) aTTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATc < 1:268255/149‑1 (MQ=255) tgaaaaGGCGCCGAAGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAg > 4:36197/3‑149 (MQ=255) aaaaaGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAgg > 3:265395/1‑149 (MQ=255) aaaaGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGc < 8:220354/149‑1 (MQ=255) aaaaGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGc < 8:293377/149‑1 (MQ=255) aaaaGGCGCCGAAGCGCCTTTTTAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAg < 3:77044/125‑1 (MQ=255) aaaaGGCGCCGAAGCGCCTTTTTAGTTTGCAGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAg > 4:77044/1‑125 (MQ=255) aGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTg > 2:258777/1‑107 (MQ=255) aGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTg < 1:258777/107‑1 (MQ=255) ggCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAAc > 3:80416/1‑149 (MQ=255) ggCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAAc > 5:39753/1‑149 (MQ=255) gcTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACtt > 5:64871/1‑148 (MQ=255) cTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCa < 4:107640/149‑1 (MQ=255) ttCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCAc > 6:34089/1‑149 (MQ=255) cGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCAGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCAc < 7:345226/147‑1 (MQ=255) | CAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCAC > NC_000913/3525298‑3525584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |