Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,927,717	C→T	5.1%	A188V (GCG→GTG)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,717	0	C	T	5.1%	251.2 / 2.1	78	A188V (GCG→GTG)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base C (37/37);  new base T (2/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01

CGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATA  >  NC_000913/3927570‑3927860                                                                                                                                                    |                                                                                                                                                cGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCTAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCg                                                                                                                                                <  6:189994/149‑1 (MQ=255)    tCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGa                                                                                                                                               >  1:142817/1‑147 (MQ=255)        ggCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGc                                                                                                                                           <  1:80195/147‑1 (MQ=255)        ggCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCg                                                                                                                                          >  3:119043/1‑148 (MQ=255)        ggCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCg                                                                                                                                          <  5:213188/148‑1 (MQ=255)         gCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGaa                                                                                                                                        <  6:25269/149‑1 (MQ=255)               aTTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGAGAGCGAAAGATCt                                                                                                                                  >  4:215177/1‑149 (MQ=255)                ttATAGCAACCGAAGCTGCGGTTAGAGAAGAGTTTGGCCTGGCACCGTTCCTGGCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGAGCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCtt                                                                                                                                 <  3:114581/149‑1 (MQ=255)                  aaaGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTg                                                                                                                                <  3:321486/148‑1 (MQ=255)                   aaGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgg                                                                                                                               <  3:17361/148‑1 (MQ=255)                      cAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGcgcg                                                                                                                           <  5:363979/149‑1 (MQ=255)                         ccGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTa                                                                                                                        >  4:14768/1‑149 (MQ=255)                           gAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTAtt                                                                                                                      >  3:257015/1‑149 (MQ=255)                                 gCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGt                                                                                                                >  3:68696/1‑149 (MQ=255)                                         cGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTg                                                                                                        >  7:280121/1‑149 (MQ=255)                                         cGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTg                                                                                                        <  4:68696/149‑1 (MQ=255)                                         cGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTg                                                                                                        >  7:369134/1‑149 (MQ=255)                                          gAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTgg                                                                                                       >  8:280414/1‑149 (MQ=255)                                             gagTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggcgg                                                                                                    <  2:142817/149‑1 (MQ=255)                                                tttGGCCTCTCACCGTTCCTGCCGGATAAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGTCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggcggc                                                                                                   >  7:60659/1‑147 (MQ=255)                                                    gCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCt                                                                                              <  3:151417/148‑1 (MQ=255)                                                     ccTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGa                                                                                            <  7:161964/149‑1 (MQ=255)                                                      cTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAg                                                                                           >  3:309275/1‑149 (MQ=255)                                                       tGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGc                                                                                          >  5:268122/1‑149 (MQ=255)                                                       tGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGc                                                                                          >  7:107229/1‑149 (MQ=255)                                                          agccGTTCCTGCCGGATCAGATCGACTTCGTACAAAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGTGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGAt                                                                                       <  2:312533/147‑1 (MQ=255)                                                           aCCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATg                                                                                      <  2:357228/149‑1 (MQ=255)                                                           aCCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTACTTGTCGGGATTGGCGGCCAGCTGAGCGATg                                                                                      >  2:333348/1‑149 (MQ=255)                                                            ccGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATgg                                                                                     <  3:215177/149‑1 (MQ=255)                                                                       ggATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCac                                                                           >  6:33957/1‑148 (MQ=255)                                                                            aGATCCACTTCGTACACAGCCAGCAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTg                                                                     >  8:146274/1‑149 (MQ=255)                                                                             gATGCACTTCGTACTCAGGCTGGAGTTACTGTCGCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgc                                                                    <  2:357901/149‑1 (MQ=255)                                                                             gATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgc                                                                    >  6:158911/1‑149 (MQ=255)                                                                             gATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgc                                                                    <  2:352841/149‑1 (MQ=255)                                                                             gATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgc                                                                    >  5:167720/1‑149 (MQ=255)                                                                              aTCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcg                                                                   >  7:46638/1‑149 (MQ=255)                                                                                   cTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCAc                                                              <  2:43461/149‑1 (MQ=255)                                                                                           acaGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTat                                                      >  2:253315/1‑149 (MQ=255)                                                                                             aGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatga                                                    >  6:125439/1‑149 (MQ=255)                                                                                              gCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatgat                                                   <  5:33957/149‑1 (MQ=255)                                                                                              gCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatgat                                                   <  8:123697/149‑1 (MQ=255)                                                                                               ccAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatgat                                                   >  2:365100/1‑148 (MQ=255)                                                                                                cAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCCCGACGTGCGCGCACCGGATTAtgatga                                                 >  4:49929/1‑149 (MQ=255)                                                                                                cAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTAtgatga                                                 <  8:107229/149‑1 (MQ=255)                                                                                                   gAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTg                                              <  1:253315/149‑1 (MQ=255)                                                                                                   gAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTg                                              <  8:369134/149‑1 (MQ=255)                                                                                                     gTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGa                                            <  4:257015/149‑1 (MQ=255)                                                                                                      ttACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGAGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAg                                           >  6:92278/1‑149 (MQ=255)                                                                                                          tGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAcc                                       >  7:116011/1‑149 (MQ=255)                                                                                                           gTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAccc                                      >  6:94498/1‑149 (MQ=255)                                                                                                            tctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGTGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAcccc                                     >  6:358383/1‑149 (MQ=255)                                                                                                            tctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGcc                                                                             >  7:201369/1‑109 (MQ=255)                                                                                                            tctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGcc                                                                             <  8:201369/109‑1 (MQ=255)                                                                                                            tctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAcccc                                     <  3:14768/149‑1 (MQ=255)                                                                                                              tcGTTATCCGGATCTTGATGCCAAAGGTCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGt                                   >  2:124585/1‑149 (MQ=255)                                                                                                                gTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCg                                    <  4:119043/146‑1 (MQ=255)                                                                                                                gTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGt                                   >  4:330696/1‑147 (MQ=255)                                                                                                                gTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCa                                 <  8:46638/149‑1 (MQ=255)                                                                                                                    tCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGa                                                                                            <  6:169244/86‑1 (MQ=255)                                                                                                                    tCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGa                                                                                            >  5:169244/1‑86 (MQ=255)                                                                                                                    tCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGc                             >  6:314797/1‑149 (MQ=255)                                                                                                                     ccGGATCTTGATTCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCt                            >  8:130204/1‑149 (MQ=255)                                                                                                                     ccGGATCTTGATGCCAAAGGGCGTGAGCGGGTGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTAtgatga                                                 <  1:191258/128‑1 (MQ=255)                                                                                                                     ccGGATCTTGATGCCAAAGGGCGTGAGCGGGTGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTAtgatga                                                 >  2:191258/1‑128 (MQ=255)                                                                                                                      cGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTg                           <  6:167720/149‑1 (MQ=255)                                                                                                                      cGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGCATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGCGCACCCCGACAGAGCTg                           >  4:9558/1‑149 (MQ=255)                                                                                                                         aTCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGc                        >  5:171578/1‑149 (MQ=255)                                                                                                                             tGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTAGTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATg                    >  1:167170/1‑149 (MQ=255)                                                                                                                              gATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCa                      <  1:365100/146‑1 (MQ=255)                                                                                                                                tGCCAAAGGGCGTGAGCGGGTGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCgg                 <  5:358383/149‑1 (MQ=255)                                                                                                                                tGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCgg                 <  8:280121/149‑1 (MQ=255)                                                                                                                                 gcccacgggCGGGAGCGGGCGATAGCGTAAGATCTGGGCGCGGTATTCCTTGGCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGGGCGAGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCgg                 <  3:9558/142‑1 (MQ=255)                                                                                                                                  ccAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTgg                          >  2:162010/1‑138 (MQ=255)                                                                                                                                  ccAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTgg                          <  1:162010/138‑1 (MQ=255)                                                                                                                                   cAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTc              <  4:309275/149‑1 (MQ=255)                                                                                                                                    aaaGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGt               <  8:144225/147‑1 (MQ=255)                                                                                                                                    aaaGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGt               >  7:144225/1‑147 (MQ=255)                                                                                                                                               gCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCATTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGata  <  7:130204/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                CGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATA  >  NC_000913/3927570‑3927860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑