Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,930,341 A→C 5.2% R251R (CGT→CGG ravA ← hexameric AAA+ MoxR family ATPase, putative molecular chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,930,3410AC 5.2% 293.5 / 4.4 93R251R (CGT→CGGravAhexameric AAA+ MoxR family ATPase, putative molecular chaperone
Reads supporting (aligned to +/- strand):  ref base A (47/41);  new base C (3/2);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

TACATCAATTTGTTGTTGTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCA  >  NC_000913/3930194‑3930488
                                                                                                                                                   |                                                                                                                                                    
tACATCAATTTGTTGTTGTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGcgac                                                                                                                                                     >  3:343517/1‑149 (MQ=255)
   aTCAATTTGTTGTTGTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGCCCGCTAAAAACGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGAt                                                                                                                                                  >  5:226050/1‑149 (MQ=255)
   aTCAATTTGTTGTTGTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGAt                                                                                                                                                  >  3:319556/1‑149 (MQ=255)
     cAATTTGTTGTTGTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATcc                                                                                                                                                >  5:217316/1‑149 (MQ=255)
      aaTTTGTTGTTGTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATcc                                                                                                                                                <  7:307770/148‑1 (MQ=255)
      aaTTTGTTGTTGTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATcc                                                                                                                                                <  7:150061/148‑1 (MQ=255)
                  tATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATc                                                                                                                                                 >  8:159325/1‑135 (MQ=255)
                  tATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATc                                                                                                                                                 <  7:159325/135‑1 (MQ=255)
                  tATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGgcg                                                                                                                                   >  3:16299/1‑149 (MQ=255)
                  tATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGgcg                                                                                                                                   >  2:371345/1‑149 (MQ=255)
                    tCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGgc                                                                                                                                    >  3:111052/1‑146 (MQ=255)
                    tCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGgc                                                                                                                                    <  4:111052/146‑1 (MQ=255)
                    tCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCa                                                                                                                                 >  2:373378/1‑149 (MQ=255)
                     cAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCAt                                                                                                                                >  4:214963/1‑149 (MQ=255)
                         ttCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGCCCGCTAAAAACGGCGCTGGCCGGCAATAAACGGATCGCTTTTTTCCCGCGCCGAGCCGCGCCATAAGCCGCATCCgg                                                                                                                            >  7:301439/1‑149 (MQ=255)
                         ttCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCgg                                                                                                                            <  1:352415/149‑1 (MQ=255)
                            aGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTaa                                                                                                                         <  3:179090/149‑1 (MQ=255)
                              gCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAAtt                                                                                                                       >  3:116378/1‑149 (MQ=255)
                                     gcgTCATACCACAGGCAATCTTTCAGGAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGAAATAATCGGCTCGCTTTTTTCCAGAGAGGATCCGAGACTTAAGGGGCATCCGGTAATTTTTCTAg                                                                                                                >  2:234043/1‑149 (MQ=255)
                                       gTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGCCGATCCGAGACATAAGGCGCATCCGGTAATTTATcc                                                                                                                  <  5:213282/145‑1 (MQ=255)
                                       gTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGCCGATCCGAGACATAAGGCGCATCCGGTAATTTATcc                                                                                                                  >  6:213282/1‑145 (MQ=255)
                                       gTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGCCGATCCGAGACATAAGGCGCATCCGGTAATTTATcc                                                                                                                  <  5:208083/145‑1 (MQ=255)
                                       gTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGCCGATCCGAGACATAAGGCGCATCCGGTAATTTATcc                                                                                                                  >  6:208083/1‑145 (MQ=255)
                                            aCCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTgg                                                                                                         >  3:90228/1‑149 (MQ=255)
                                              cacaGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCgag                                                                                                                                             <  3:199629/111‑1 (MQ=255)
                                              cacaGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCgag                                                                                                                                             >  4:199629/1‑111 (MQ=255)
                                              cacaGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGgcg                                                                                                       >  4:284317/1‑149 (MQ=255)
                                                 aGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGATGGCCTGCAATAATCGGATCGCTTTTTTACAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCgcag                                                                                                    >  1:192437/1‑149 (MQ=255)
                                                  ggCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCgcagc                                                                                                   <  6:226050/149‑1 (MQ=255)
                                                  ggCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCgcagc                                                                                                   <  3:284317/149‑1 (MQ=255)
                                                   gCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCgcagca                                                                                                  >  8:95889/1‑149 (MQ=255)
                                                     aaTCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATa                                                                                               <  4:343517/149‑1 (MQ=255)
                                                     aaTCTTTCAGCAAAATGAGATCTACCGGGGAAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTATTTTCCAGCGACGATCAGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCAt                                                                                                 >  6:80772/1‑148 (MQ=255)
                                                               cAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTACAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCAAGTTGCTGGCGCAGCATAAAAATCAGc                                                                                      >  7:79772/1‑148 (MQ=255)
                                                                aaaaTGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGGTc                                                                                    <  4:90228/149‑1 (MQ=255)
                                                                aaaaTGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTc                                                                                    <  6:217316/149‑1 (MQ=255)
                                                                aaaaTGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTc                                                                                    <  7:48311/149‑1 (MQ=255)
                                                                   aTGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCaaa                                                                                 >  3:154511/1‑149 (MQ=255)
                                                                   aTGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCaaa                                                                                 <  8:301810/149‑1 (MQ=255)
                                                                    tGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCaaa                                                                                 >  5:319140/1‑148 (MQ=255)
                                                                    tGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCaaa                                                                                 >  8:219717/1‑148 (MQ=255)
                                                                       gaTCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACa                                                                             <  8:9690/149‑1 (MQ=255)
                                                                       gaTCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACa                                                                             <  1:372858/149‑1 (MQ=255)
                                                                        aTCTACCGGGGCAACAGCCCTGCGTCCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACa                                                                             <  8:301439/148‑1 (MQ=255)
                                                                           tACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATc                                                                                         >  6:7987/1‑133 (MQ=255)
                                                                           tACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATc                                                                                         <  5:7987/133‑1 (MQ=255)
                                                                                       aGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTaa                                                             >  4:244975/1‑149 (MQ=255)
                                                                                           cTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAAttt                                                          >  7:197422/1‑148 (MQ=255)
                                                                                            tGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTaa                                                             <  7:89556/144‑1 (MQ=255)
                                                                                            tGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTaa                                                             >  8:89556/1‑144 (MQ=255)
                                                                                              cGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCAc                                                       <  7:154658/148‑1 (MQ=255)
                                                                                               gACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCAcc                                                      >  7:348349/1‑148 (MQ=255)
                                                                                                 ccGCTATAAAAGGCGCTGGCCGGCAATAATCCGATGGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTATTTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAAt                                                   <  6:47403/149‑1 (MQ=255)
                                                                                                 ccGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCa                                                                                                                 <  7:274572/88‑1 (MQ=255)
                                                                                                 ccGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCa                                                                                                                 >  8:274572/1‑88 (MQ=255)
                                                                                                 ccGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAAt                                                   <  4:16299/149‑1 (MQ=255)
                                                                                                 ccGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAAt                                                   <  7:157183/149‑1 (MQ=255)
                                                                                                  cGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAAtc                                                  <  1:371345/149‑1 (MQ=255)
                                                                                                     tAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCgcagc                                                                                                   >  5:74801/1‑98 (MQ=255)
                                                                                                     tAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCgcagc                                                                                                   <  6:74801/98‑1 (MQ=255)
                                                                                                     tAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCAc                                                       >  8:213468/1‑141 (MQ=255)
                                                                                                     tAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCAc                                                       <  7:213468/141‑1 (MQ=255)
                                                                                                     tAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGCGCAGCGTAATTTCACCAATctct                                               >  3:223835/1‑149 (MQ=255)
                                                                                                       aaacaGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCttt                                             <  2:192437/149‑1 (MQ=255)
                                                                                                       aaaaaGGCGCTGGCCTGCAATTATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCa                                                                   <  8:252715/127‑1 (MQ=255)
                                                                                                         aaaGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCt                                           <  4:116378/149‑1 (MQ=255)
                                                                                                           aGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGc                                         <  4:154511/149‑1 (MQ=255)
                                                                                                               gcTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGc                                     >  7:105227/1‑149 (MQ=255)
                                                                                                                 tGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATc                                                                                         <  8:209858/95‑1 (MQ=255)
                                                                                                                   gCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGtt                                  >  3:252558/1‑148 (MQ=255)
                                                                                                                   gCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTc                                 <  8:105227/149‑1 (MQ=255)
                                                                                                                     cTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCa                                >  3:295600/1‑148 (MQ=255)
                                                                                                                     cTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCa                                >  4:85055/1‑148 (MQ=255)
                                                                                                                      tGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCata                              >  3:123194/1‑149 (MQ=255)
                                                                                                                      tGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCata                              >  3:247990/1‑149 (MQ=255)
                                                                                                                      tGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCata                              >  2:177979/1‑149 (MQ=255)
                                                                                                                        cAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCata                              <  1:373378/147‑1 (MQ=255)
                                                                                                                         aataatCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAAGCTCTTTCTGCCAGCGTTCatat                             >  4:50678/1‑148 (MQ=255)
                                                                                                                           taatCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCttt                                             >  1:143327/1‑129 (MQ=255)
                                                                                                                           taatCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCttt                                             <  2:143327/129‑1 (MQ=255)
                                                                                                                           taatCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTc                                            <  8:184213/130‑1 (MQ=255)
                                                                                                                           taatCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTc                                            >  1:1266/1‑130 (MQ=255)
                                                                                                                           taatCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTc                                            <  2:1266/130‑1 (MQ=255)
                                                                                                                           taatCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTc                                            >  7:184213/1‑130 (MQ=255)
                                                                                                                           taatCGGATCGCTTTTTTCCAGCGACGATCCGAGACAGAAGTCGCAACATTTAAATTATCCAGATGCAGCCGCAGCAAAAAAATCAGCTCAAATACATCCTCGGGCAGCGTAATTTCACCAAACTCTTTCAGCCAGCGTTCATAatcat                         >  4:324603/1‑147 (MQ=255)
                                                                                                                                       ttttttCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGgcg                                                                                                       <  3:63280/60‑1 (MQ=255)
                                                                                                                                       ttttttCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGgcg                                                                                                       >  4:63280/1‑60 (MQ=255)
                                                                                                                                         ttttCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCt             <  1:30676/147‑1 (MQ=255)
                                                                                                                                           ttCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGc           >  4:47609/1‑147 (MQ=255)
                                                                                                                                               aGCGACGTTCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCaa         <  4:96656/145‑1 (MQ=255)
                                                                                                                                               aGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAgag      >  3:96656/1‑146 (MQ=255)
                                                                                                                                               aGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCa     >  6:254632/1‑149 (MQ=255)
                                                                                                                                                gCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCAt    <  6:122278/149‑1 (MQ=255)
                                                                                                                                                gCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCAt    >  5:211546/1‑149 (MQ=255)
                                                                                                                                                  gacgaTCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCa  <  4:319556/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TACATCAATTTGTTGTTGTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCA  >  NC_000913/3930194‑3930488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: