Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,367,339 G→A 5.2% P330L (CCG→CTG)  aspA ← aspartate ammonia‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,367,3390GA 5.2% 247.3 / 2.5 76P330L (CCG→CTG) aspAaspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base G (41/31);  new base A (2/2);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

AGAATGTGAACGGATTCGAACATGGCCTGGCCAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCG  >  NC_000913/4367192‑4367485
                                                                                                                                                   |                                                                                                                                                  
aGAATGTGAACGGATTCGAACATGGCCTGGCCAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCgg                                                                                                                                                   <  1:154115/149‑1 (MQ=255)
  aaTGTGAACGGATTCGAACATGGCCTGGCCAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGt                                                                                                                                                 <  7:355289/149‑1 (MQ=255)
    tgtgAACGGATTCGAACATGGCCTGGCCAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCGATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGtt                                                                                                                                                >  6:111537/1‑148 (MQ=255)
      tgAACGGATTCGAACATGGCCTGGCCAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATTACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTAc                                                                                                                                             <  5:24909/149‑1 (MQ=255)
             aTTCGAACATGGCCTGGCCAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGAAGTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCt                                                                                                                                      >  6:232774/1‑149 (MQ=255)
                 gAACATGGCCTGGCCAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTa                                                                                                                                         <  2:307961/142‑1 (MQ=255)
                 gAACATGGCCTGGCCAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGACGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTa                                                                                                                                         >  1:307961/1‑142 (MQ=255)
                     aTGGCCTGGCCAATGACCGGCTCCATAACGTTCATCTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTTAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCatgat                                                                                                                              <  7:151103/149‑1 (MQ=255)
                      tggcctggccAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCatgatg                                                                                                                             <  7:55149/149‑1 (MQ=255)
                         cctggccAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCAGGTTTACTTTAGCTGGCATGATgg                                                                                                                            >  8:275565/1‑147 (MQ=255)
                         cctggccAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCAGGTTTACTTTAGCTGGCATGATgg                                                                                                                            >  4:138906/1‑147 (MQ=255)
                          ctggccAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATgg                                                                                                                            >  8:61483/1‑146 (MQ=255)
                             gccAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGc                                                                                                                      >  7:150534/1‑149 (MQ=255)
                               cAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGccc                                                                                                                    >  6:46890/1‑149 (MQ=255)
                                aaTGACCGGCTAAATAACGTTCAACTGCAGCTGACCTGCGTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGCAGCATACCTGGTTAAACACTACCGGAACACCCGGGTTTACTTTAGCTGGCATGATGTAAGCGCCCg                                                                                                                   >  5:120915/1‑149 (MQ=255)
                                     ccGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTg                                                                                                               >  1:194310/1‑148 (MQ=255)
                                       ggCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAg                                                                                                            >  1:363979/1‑149 (MQ=255)
                                            cATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCg                                                                                                       >  8:130757/1‑149 (MQ=255)
                                             aTAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCgg                                                                                                      <  2:194310/149‑1 (MQ=255)
                                              tAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCgg                                                                                                      >  5:238044/1‑148 (MQ=255)
                                               aaCGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGaa                                                                                                                          <  5:78994/127‑1 (MQ=255)
                                               aaCGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGaa                                                                                                                          >  6:78994/1‑127 (MQ=255)
                                                   ttCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGCGCCCGCCTGCAGTTCCGGCAGGtt                                                                                                >  5:164596/1‑149 (MQ=255)
                                                            aGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGtt                                                                                       <  5:12959/149‑1 (MQ=255)
                                                            aGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGt                                                                                        >  4:298368/1‑148 (MQ=255)
                                                                 aCCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGGGCCCGCCTGCAGTTc                                                                                                         >  4:49048/1‑126 (MQ=255)
                                                                 aCCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGGTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGc                                                                                  <  6:248482/149‑1 (MQ=255)
                                                                   cTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCg                                                                                >  1:333753/1‑149 (MQ=255)
                                                                   cTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGGTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCg                                                                                >  6:336009/1‑149 (MQ=255)
                                                                    tGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCgg                                                                               >  1:180646/1‑149 (MQ=255)
                                                                    tGCGTCTGCTGCCATGGTAACAGTGGTGTCGGTACCGATGACTGTGCCGCATACCTGGTTAACCACGTCCGGAACAACCGGGGGGACGTTAGCTGGCCGGAGGGAAGAGCCCGCCTGAAGTGACGGCAGGTGGAGCGCGGTccggccgg                                                                               >  6:159268/1‑149 (MQ=255)
                                                                      cgtCTGCGGCCAGGGGACCAGTGGGGGCGTTACCGATGACTTTGAAGCATACCTGGGTCACCACTTCCGGAACCACCGGGGTTACTTTAGCTGGCCTGAGGGAAGAGCCCGCCGGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCg                                                                                <  5:159268/144‑1 (MQ=255)
                                                                      cTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCa                                                                             >  4:45565/1‑149 (MQ=255)
                                                                            ctgcCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGttg                                                                        <  2:190945/148‑3 (MQ=255)
                                                                            ctgcCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTgg                                                                        <  2:363979/148‑1 (MQ=255)
                                                                               ccATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGcc                                                                     <  8:120083/148‑1 (MQ=255)
                                                                               ccATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGcc                                                                     <  5:46890/148‑1 (MQ=255)
                                                                                  tGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTg                                                                   >  3:38958/1‑147 (MQ=255)
                                                                                  tGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGaa                                                                 <  7:130757/149‑1 (MQ=255)
                                                                                    gTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGACCAACCGGGTTTACTTTAGCTGGCAGGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAaga                                                               <  5:111537/149‑1 (MQ=255)
                                                                                       aCAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAagagag                                                            >  7:233678/1‑149 (MQ=255)
                                                                                       aCAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAagaga                                                             <  2:333753/148‑1 (MQ=255)
                                                                                       aCAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCAGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAagaga                                                             <  3:138906/148‑1 (MQ=255)
                                                                                       aCAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCAGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAagaga                                                             <  7:275565/148‑1 (MQ=255)
                                                                                         aGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACtt                                                                                                                                           >  4:292454/1‑68 (MQ=255)
                                                                                         aGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACtt                                                                                                                                           <  3:292454/68‑1 (MQ=255)
                                                                                         aGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGc                                                                      >  6:199790/1‑137 (MQ=255)
                                                                                         aGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGc                                                                      <  5:199790/137‑1 (MQ=255)
                                                                                          gtggtgTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCaa                                                         <  7:61483/149‑1 (MQ=255)
                                                                                              tgtCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAgcg                                                      >  3:154605/1‑148 (MQ=255)
                                                                                                tCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAg                                                   >  5:331413/1‑149 (MQ=255)
                                                                                                tCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAg                                                   <  3:45565/149‑1 (MQ=255)
                                                                                                    tACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCg                                               >  3:23911/1‑149 (MQ=255)
                                                                                                      ccGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGt                                              >  3:182749/1‑148 (MQ=255)
                                                                                                        gATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCTCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTa                                            >  7:260413/1‑148 (MQ=255)
                                                                                                               ttGCAGCATACCTGGTTAACCTCTTCAGGAACAACGGTGTTTACTTTAGCTGTCATGATGGAAGAGGCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGACTGAAGAGAGCAAGCGCAGGTCGTTACAGAttt                                     <  2:113883/148‑1 (MQ=255)
                                                                                                                tGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGAttt                                     <  4:38958/147‑1 (MQ=255)
                                                                                                                tGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTg                                   >  3:137982/1‑149 (MQ=255)
                                                                                                                         ccTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCtt                           >  8:185945/1‑148 (MQ=255)
                                                                                                                         ccTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTc                          <  4:154605/149‑1 (MQ=255)
                                                                                                                         ccTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTc                          <  6:238044/149‑1 (MQ=255)
                                                                                                                             gTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCTCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTcac                        <  8:260413/147‑1 (MQ=255)
                                                                                                                               tAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGc                     >  7:328815/1‑148 (MQ=255)
                                                                                                                                 aCCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCa                   <  5:232774/148‑1 (MQ=255)
                                                                                                                                 aCCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAg                  <  8:150534/149‑1 (MQ=255)
                                                                                                                                  ccACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAgg                 >  7:286692/1‑149 (MQ=255)
                                                                                                                                  ccACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAgg                 >  7:113050/1‑149 (MQ=255)
                                                                                                                                   cACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGc                <  7:185945/149‑1 (MQ=255)
                                                                                                                                   cACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGc                >  4:20835/1‑149 (MQ=255)
                                                                                                                                      ttCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCa                                                                                     <  2:348182/77‑1 (MQ=255)
                                                                                                                                       tCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGcc                    >  1:61761/1‑141 (MQ=255)
                                                                                                                                       tCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGGGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGcc                    <  2:61761/141‑1 (MQ=255)
                                                                                                                                        ccGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGttt            >  1:216489/1‑148 (MQ=255)
                                                                                                                                          ggAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAg         >  5:92543/1‑149 (MQ=255)
                                                                                                                                           gAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAgc        >  7:1359/1‑149 (MQ=255)
                                                                                                                                               aacCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAgcgcgc    >  8:62155/1‑149 (MQ=255)
                                                                                                                                                 ccGGGTTTACTTTAGCTGGCATGATGGAAGCGCCCGCCTGCAGTTCCGGCAGGTTGATCACGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCCGCGCGCCg  >  1:293778/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGAATGTGAACGGATTCGAACATGGCCTGGCCAATGACCGGCTCCATAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCG  >  NC_000913/4367192‑4367485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑