Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,010,169 C→T 5.0% A69V (GCG→GTG)  uup → replication regulatory ABC‑F family DNA‑binding ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,010,1690CT 5.0% 256.2 / 2.0 79A69V (GCG→GTG) uupreplication regulatory ABC‑F family DNA‑binding ATPase
Reads supporting (aligned to +/- strand):  ref base C (35/40);  new base T (2/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

GATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAAC  >  NC_000913/1010021‑1010317
                                                                                                                                                    |                                                                                                                                                    
gATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAgc                                                                                                                                                      <  1:24493/149‑1 (MQ=255)
gATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAgc                                                                                                                                                      >  7:293342/1‑149 (MQ=255)
gATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAgc                                                                                                                                                      >  4:306110/1‑149 (MQ=255)
  tAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAgcgc                                                                                                                                                    <  8:283980/149‑1 (MQ=255)
  tAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAgcgc                                                                                                                                                    <  4:208383/149‑1 (MQ=255)
    aCGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCACCCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGt                                                                                                                                                  <  2:224453/149‑1 (MQ=255)
      gCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCt                                                                                                                                                <  3:306110/149‑1 (MQ=255)
      gCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCt                                                                                                                                                >  1:9331/1‑149 (MQ=255)
       cAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTg                                                                                                                                               <  8:16227/149‑1 (MQ=255)
        aGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTg                                                                                                                                               >  4:208864/1‑148 (MQ=255)
            cTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACa                                                                                                                                          <  7:61141/149‑1 (MQ=255)
               cATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGATGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGa                                                                                                                                       >  6:205332/1‑149 (MQ=255)
                atatCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGAt                                                                                                                                      >  4:88010/1‑149 (MQ=255)
                 tatCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATc                                                                                                                                     <  6:336319/149‑1 (MQ=255)
                 tatCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATc                                                                                                                                     >  6:207225/1‑149 (MQ=255)
                 tatCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATc                                                                                                                                     <  8:340421/149‑1 (MQ=255)
                  atCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATcc                                                                                                                                    >  6:207265/1‑149 (MQ=255)
                   tCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATccg                                                                                                                                   >  4:162383/1‑149 (MQ=255)
                       aGATAACGACCGTGGTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACAGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCcgccgc                                                                                                                               <  8:153639/149‑1 (MQ=255)
                        gATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCcgcg                                                                                                                              >  5:26937/1‑149 (MQ=255)
                           aacgaacgTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTaa                                                                                                                           >  8:12054/1‑149 (MQ=255)
                                 cgTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGa                                                                                                                     <  1:315008/149‑1 (MQ=255)
                                  gtgtTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAg                                                                                                                    <  2:128114/149‑1 (MQ=255)
                                   tgtTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAgg                                                                                                                   <  3:142028/149‑1 (MQ=255)
                                         tCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTAAACCGTGATCAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATGTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGCGTAGCg                                                                                                             >  5:319691/1‑149 (MQ=255)
                                            ggtggGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCaa                                                                                                                                            >  6:329936/1‑115 (MQ=255)
                                            ggtggGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCaa                                                                                                                                            <  5:329936/115‑1 (MQ=255)
                                             gtggGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGttt                                                                                                          <  1:151515/148‑1 (MQ=255)
                                             gtggGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTa                                                                                                         <  3:162383/149‑1 (MQ=255)
                                              tggGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGAATGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTAt                                                                                                        <  1:88131/149‑1 (MQ=255)
                                              tggGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGCATGACGGTCGCATTATTAACGAGCAAGATATGATTGAAGCGCGTCTGCAACAGGATCCCCCGCCTAACGATGAGGGTAGCGATTAt                                                                                                        >  6:174519/1‑149 (MQ=255)
                                                  ccGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGAtt                                                                                                    >  8:267084/1‑149 (MQ=255)
                                                   cGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGAttt                                                                                                   <  2:347865/149‑1 (MQ=255)
                                                       aCGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGtt                                                                                               <  8:169742/149‑1 (MQ=255)
                                                        cGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTg                                                                                              <  3:88010/149‑1 (MQ=255)
                                                        cGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTg                                                                                              <  5:6638/149‑1 (MQ=255)
                                                            gcAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCg                                                                                           >  3:185261/1‑148 (MQ=255)
                                                             cAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGaa                                                                                         <  4:252878/149‑1 (MQ=255)
                                                                 cAAATCGATGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCa                                                                                     <  5:205332/149‑1 (MQ=255)
                                                                  aaaTCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCAt                                                                                    >  3:16660/1‑149 (MQ=255)
                                                                  aaaTCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCAt                                                                                    >  1:42339/1‑149 (MQ=255)
                                                                   aaTCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGTGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCAtt                                                                                   >  7:112763/1‑149 (MQ=255)
                                                                   aaTCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGTGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCAtt                                                                                   >  7:118030/1‑149 (MQ=255)
                                                                   aaTCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCAtt                                                                                   <  1:355272/149‑1 (MQ=255)
                                                                    aTCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTg                                                                                  <  8:293342/149‑1 (MQ=255)
                                                                       gACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTgaag                                                                               <  7:267084/149‑1 (MQ=255)
                                                                       gACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTgaa                                                                                <  5:207265/148‑1 (MQ=255)
                                                                        aCGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTgaaga                                                                              <  3:17549/149‑1 (MQ=255)
                                                                           ttAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTGGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAAc                                                                            >  3:65317/1‑148 (MQ=255)
                                                                               tGAAAATCCTCAACCGTGAACAAGGGCTGGATGCCGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCg                                                                       >  1:60794/1‑149 (MQ=255)
                                                                               tGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCg                                                                       <  5:135307/149‑1 (MQ=255)
                                                                               tGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCg                                                                       >  3:51714/1‑149 (MQ=255)
                                                                               tGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCg                                                                       >  6:135307/1‑149 (MQ=255)
                                                                                        tCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGc                                                                        <  5:14054/139‑1 (MQ=255)
                                                                                        tCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGc                                                                        >  6:14054/1‑139 (MQ=255)
                                                                                        tCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCt                                                               >  5:21093/1‑148 (MQ=255)
                                                                                              gTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAg                                                                                                                    >  8:367508/1‑89 (MQ=255)
                                                                                              gTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAg                                                                                                                    <  7:367508/89‑1 (MQ=255)
                                                                                                   cAAGGGCTGGATGACTGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCa                                                   >  1:59903/1‑149 (MQ=255)
                                                                                                     aGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCAc                                                  >  4:59569/1‑148 (MQ=255)
                                                                                                      gggCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGTGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGa                                                <  8:112763/149‑1 (MQ=255)
                                                                                                      gggCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGTGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGa                                                <  8:118030/149‑1 (MQ=255)
                                                                                                      gggCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGa                                                >  6:63728/1‑149 (MQ=255)
                                                                                                            gATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTc                                          >  8:292319/1‑149 (MQ=255)
                                                                                                              tGACGGTCGCATTATTTACGAGCAAGATTTGATTGTGGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTcgc                                        <  4:65317/149‑1 (MQ=255)
                                                                                                              tGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGCTTTCGTTGCGGAAGGCATTGAAGAActt                                                                          >  8:274516/1‑113 (MQ=255)
                                                                                                                       cATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCt                                                               >  2:312584/1‑117 (MQ=255)
                                                                                                                       cATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCt                                                               <  1:312584/117‑1 (MQ=255)
                                                                                                                        attattTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTgaagaa                                                                             <  3:109378/102‑1 (MQ=255)
                                                                                                                        attattTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTgaagaa                                                                             >  4:109378/1‑102 (MQ=255)
                                                                                                                        attattTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTgaaga                                                                              >  7:70828/1‑101 (MQ=255)
                                                                                                                        attattTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTgaaga                                                                              <  8:70828/101‑1 (MQ=255)
                                                                                                                            tttACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGaa                           <  4:16660/148‑1 (MQ=255)
                                                                                                                               aCGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGa                        <  6:26937/148‑1 (MQ=255)
                                                                                                                               aCGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGAc                       >  1:177055/1‑149 (MQ=255)
                                                                                                                                    cAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGtgat                              <  4:35324/137‑1 (MQ=255)
                                                                                                                                    cAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGtgat                              >  3:35324/1‑137 (MQ=255)
                                                                                                                                     aaGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTa                                                                                                         <  4:149747/61‑1 (MQ=255)
                                                                                                                                     aaGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTa                                                                                                         >  3:149747/1‑61 (MQ=255)
                                                                                                                                       gATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCcgagc                 <  6:172804/147‑1 (MQ=255)
                                                                                                                                       gATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCcgagc                 >  5:172804/1‑147 (MQ=255)
                                                                                                                                        aTTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCcgagcgag              <  5:207225/149‑1 (MQ=255)
                                                                                                                                         tttGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTATCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCTGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCgaga             <  5:174519/149‑1 (MQ=255)
                                                                                                                                             aTTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGaaaaa         <  3:59569/149‑1 (MQ=255)
                                                                                                                                                   gcgcGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGGGATGAACGACCCGAGCGAGAAAAATCTCaa   <  4:335159/149‑1 (MQ=255)
                                                                                                                                                    cgcgTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAAc  <  3:208864/149‑1 (MQ=255)
                                                                                                                                                    cgcgTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAAc  <  2:59903/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGCCGCAACGGCGCAGGCAAATCGACGTTAATGAAAATCCTCAACCGTGAACAAGGGCTGGATGACGGTCGCATTATTTACGAGCAAGATTTGATTGTAGCGCGTCTGCAACAGGATCCGCCGCGTAACGTTGAGGGTAGCGTTTATGATTTCGTTGCCGAAGGCATTGAAGAACAAGCGGAATATCTGAAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAAC  >  NC_000913/1010021‑1010317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: