Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,388,549 G→A 6.1% P88L (CCG→CTG)  tpx ← lipid hydroperoxide peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,388,5490GA 6.1% 208.4 / 2.4 65P88L (CCG→CTG) tpxlipid hydroperoxide peroxidase
Reads supporting (aligned to +/- strand):  ref base G (30/31);  new base A (2/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

CACAACGGCACGCGCTGCCAGACCTTTCAGTGGGCCATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGG  >  NC_000913/1388404‑1388695
                                                                                                                                                 |                                                                                                                                                  
cacaACGGCACGCGCTGCCAGACCTTTCAGTGGGCCATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACgg                                                                                                                                                   >  3:310757/1‑147 (MQ=255)
cacaACGGCACGCGCTGCCAGACCTTTCAGTGGGCCATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACgg                                                                                                                                                   >  8:300604/1‑147 (MQ=255)
 acaACGGCACGCGCTGCCAGACCTTTCAGTGGGCCATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACgg                                                                                                                                                   >  1:144814/1‑146 (MQ=255)
        cACGCGCTGCCAGACCTTTCAGTGGGCCATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCa                                                                                                                                         <  3:341350/149‑1 (MQ=255)
           gcgcTGCCAGACCTTTCAGTGGGCCATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACAGCAGATCGGCa                                                                                                                                         >  3:231390/1‑146 (MQ=255)
                  cAGACCTTTCAGTGGGCCATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACAGCAGATCgg                                                                                                                                           <  8:158531/137‑1 (MQ=255)
                  cAGACCTTTCAGTGGGCCATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACAGCAGATCgg                                                                                                                                           >  7:158531/1‑137 (MQ=255)
                           cAGTGGGCCATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACg                                                                                                                       <  7:300604/148‑1 (MQ=255)
                                 gCCATCAGCAATTGCCACACCGGAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAAAGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGtgttgt                                                                                                                <  6:104862/149‑1 (MQ=255)
                                  ccATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGgtg                                                                                                                    <  6:257419/144‑1 (MQ=255)
                                  ccATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGgtg                                                                                                                    >  5:257419/1‑144 (MQ=255)
                                  ccATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTc                                                                                                               >  4:180746/1‑149 (MQ=255)
                                    aTCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGAGTTGTCGa                                                                                                             >  1:309291/1‑149 (MQ=255)
                                     tCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGAt                                                                                                            >  7:277275/1‑149 (MQ=255)
                                            ttGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACAGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGtt                                                                                                     <  4:231390/149‑1 (MQ=255)
                                            ttGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCAGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGtt                                                                                                     >  1:277562/1‑149 (MQ=255)
                                               ccACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGGGATACACAGCACAACGGTGTTGTCGATCTCAGTTGc                                                                                                   >  5:44557/1‑148 (MQ=255)
                                                cacaCCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGc                                                                                                   >  4:305439/1‑147 (MQ=255)
                                                    ccGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGTAAACGGTGGTCAGACCTTCTGCGCCGCATAAACGAGACTGGGCGAAAGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTg                                                                                             <  4:237897/149‑1 (MQ=255)
                                                              gCAGAAATTCAGCGTTACGGAAAGTGGAGATGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGgt                                                                                                                     <  8:58364/115‑1 (MQ=255)
                                                              gCAGAAATTCAGCGTTACGGAAAGTGGAGATGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGgt                                                                                                                     >  7:58364/1‑115 (MQ=255)
                                                                aGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACg                                                                                 >  4:167184/1‑149 (MQ=255)
                                                                aGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACg                                                                                 >  1:104468/1‑149 (MQ=255)
                                                                       cAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGa                                                                           <  5:166045/148‑1 (MQ=255)
                                                                            ttACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATgcggc                                                                     <  3:180746/149‑1 (MQ=255)
                                                                            ttACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATgcggc                                                                     <  3:305439/149‑1 (MQ=255)
                                                                                ggAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCa                                                                  >  8:159722/1‑148 (MQ=255)
                                                                                   aaGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAaca                                                              >  8:241718/1‑149 (MQ=255)
                                                                                      tGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCg                                                           <  2:144814/149‑1 (MQ=255)
                                                                                      tGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGCTGTTGTCGATCTCAGTTGCCAGATGGTTAAACTTACGTACTGATGCGGCGCAAACCCCg                                                           >  2:314001/1‑149 (MQ=255)
                                                                                        gagaGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGgcgc                                                                   <  7:181416/139‑1 (MQ=255)
                                                                                        gagaGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGgcgc                                                                   >  8:181416/1‑139 (MQ=255)
                                                                                             ggTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCaa                                                    <  7:159722/149‑1 (MQ=255)
                                                                                               tGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCgg                                                          >  7:360508/1‑141 (MQ=255)
                                                                                               tGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCgg                                                          <  8:360508/141‑1 (MQ=255)
                                                                                                  tAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGa                                                                           >  6:150248/1‑121 (MQ=255)
                                                                                                  tAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGa                                                                           <  5:150248/121‑1 (MQ=255)
                                                                                                   aaCGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACCGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCg                                              >  2:132894/1‑149 (MQ=255)
                                                                                                    aCGTTGTTCAGAACTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCATATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGACAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCgg                                             <  6:20300/149‑1 (MQ=255)
                                                                                                      gttgttCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCggg                                            >  7:109538/1‑148 (MQ=255)
                                                                                                       ttgttCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGa                                           >  5:289881/1‑148 (MQ=255)
                                                                                                          ttCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACCTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAAt                                       <  8:358470/149‑1 (MQ=255)
                                                                                                             aGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGtt                                    >  7:85378/1‑149 (MQ=255)
                                                                                                              gACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTc                                   <  4:307921/149‑1 (MQ=255)
                                                                                                               aCCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGtt                                    >  4:227715/1‑147 (MQ=255)
                                                                                                               aCCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCa                                  <  4:70638/149‑1 (MQ=255)
                                                                                                                 cTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAg                                 >  6:171537/1‑148 (MQ=255)
                                                                                                                  ttCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCa                               >  7:263922/1‑149 (MQ=255)
                                                                                                                  ttCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCa                               >  8:167859/1‑149 (MQ=255)
                                                                                                                  ttCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCa                               >  1:182075/1‑149 (MQ=255)
                                                                                                                       cgccgcAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACttt                           <  2:182075/148‑1 (MQ=255)
                                                                                                                         ccgcAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCg                        <  7:241718/149‑1 (MQ=255)
                                                                                                                           gcAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGtt                      <  5:270475/149‑1 (MQ=255)
                                                                                                                            cAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGttt                     <  3:167184/149‑1 (MQ=255)
                                                                                                                              gAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTAc                   <  2:104468/149‑1 (MQ=255)
                                                                                                                              gAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTAc                   >  7:318348/1‑149 (MQ=255)
                                                                                                                               aaaCGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTc                                   <  1:8972/132‑1 (MQ=255)
                                                                                                                               aaaCGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTc                                   >  2:8972/1‑132 (MQ=255)
                                                                                                                                aaCGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTAccc                 >  7:37628/1‑149 (MQ=255)
                                                                                                                                      aCTGGGCGAACGGCAGATCGGCAGGGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAAc           <  6:44557/149‑1 (MQ=255)
                                                                                                                                        tGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACt          >  7:126589/1‑148 (MQ=255)
                                                                                                                                          ggCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCTGCACTTTGCGTTTACCCGCAAACTGAc       <  4:310757/149‑1 (MQ=255)
                                                                                                                                           gCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGAcc      <  8:109538/149‑1 (MQ=255)
                                                                                                                                             gAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGa    <  8:277275/149‑1 (MQ=255)
                                                                                                                                             gAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGa    <  6:289881/149‑1 (MQ=255)
                                                                                                                                             gAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGa    <  5:171537/149‑1 (MQ=255)
                                                                                                                                              aaCGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCTGATCAAACAACTTTATCAATACTCGGGAAAATGTTCAGCACTATGCGTTTCCCCGCAAACTGACCGAg   >  1:146142/1‑149 (MQ=255)
                                                                                                                                               aCGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAgg  <  7:265729/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CACAACGGCACGCGCTGCCAGACCTTTCAGTGGGCCATCAGCAATTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGG  >  NC_000913/1388404‑1388695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑