Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,419,711 T→C 6.3% intergenic (‑255/+54) ydaG ← / ← racR Rac prophage; uncharacterized protein/Rac prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,419,7110TC 6.3% 227.4 / 4.7 80intergenic (‑255/+54)ydaG/racRRac prophage; uncharacterized protein/Rac prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (44/30);  new base C (2/3);  total (46/34)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

CACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCGCTGCTATGT  >  NC_000913/1419563‑1419856
                                                                                                                                                    |                                                                                                                                                 
cACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGt                                                                                                                                                   >  1:275213/1‑149 (MQ=255)
  cTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGa                                                                                                                                                 <  7:51937/149‑1 (MQ=255)
       tGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATgggg                                                                                                                                            >  3:141969/1‑149 (MQ=255)
         gTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGGTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATgggggg                                                                                                                                          >  1:40578/1‑149 (MQ=255)
            aCTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGt                                                                                                                                        <  8:60835/148‑1 (MQ=255)
             cTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGtt                                                                                                                                       >  3:109414/1‑148 (MQ=255)
              tCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTa                                                                                                                                      >  7:331387/1‑148 (MQ=255)
              tCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGATTa                                                                                                                                      >  3:288000/1‑148 (MQ=255)
               cGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTaaa                                                                                                                                    >  3:281574/1‑149 (MQ=255)
                   gTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAAATGATTGTCAAAAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGGTAAATAAc                                                                                                                                <  4:38767/149‑1 (MQ=255)
                         cccGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGTGGGGTTAAATAACGGAATc                                                                                                                          >  7:166042/1‑149 (MQ=255)
                          ccGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATc                                                                                                                          >  3:227920/1‑148 (MQ=255)
                                  cTGGGGGGCGGGGTGGGGGGTATGCTGGAACTATAGGAAATGCCTAATTGATTGGCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATTGGTGATGGCGACAGCAGAAAGTGCTGGGGGGGTTAAATAACGGAATCCAGGAGttt                                                                                                                 <  4:195168/149‑1 (MQ=255)
                                     tgGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGtttt                                                                                                                <  8:331387/147‑1 (MQ=255)
                                     tgGGGCGGGGTGGGGGGCAAGCTGGAACTAACTGTAATCCCTAAGTCATTGTCAAAAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGCCGACACCAGAAAGCGATGGGGGGCTTAAATAACGGAATCCAGGAGTTTTcc                                                                                                              <  7:183491/149‑1 (MQ=255)
                                     gggggAGGGGTGGGTGGTCTCCTGGAACTAGAGGTAAATCCAATTTGATTGTCAATCGGCTATGCCTAATGTTTTGATCGTAACCTCATAGGTGATGTCCACAGAAGAACGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTcc                                                                                                              <  1:108230/148‑1 (MQ=255)
                                                   tggtATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCatat                                                                                                  >  5:67353/1‑147 (MQ=255)
                                                      tATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagtt                                                                                             >  7:278714/1‑149 (MQ=255)
                                                        tGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagttta                                                                                           <  8:278714/149‑1 (MQ=255)
                                                        tGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATCGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATTtaagttt                                                                                            >  5:309920/1‑148 (MQ=255)
                                                          cTGGAACAATACGTAATGCCTACTTGAAGCTCCATAGGCTATGCCTAATGTTCTGAGCGTACCCTAATAGGTGATGGCCACCGCACAAAGTGATGGGCGGGGTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagtttaag                                                                                         <  4:141969/149‑1 (MQ=255)
                                                                caaaaGGTACAGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAg                                                                                   <  4:281574/145‑1 (MQ=255)
                                                                   taGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGttt                                                                                >  6:64643/1‑149 (MQ=255)
                                                                      gTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTtg                                                                             >  1:182721/1‑149 (MQ=255)
                                                                      gTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTtg                                                                             >  7:54637/1‑149 (MQ=255)
                                                                       gttctgcTAATTGATTTTCAATATGCTTTGGCTAATGTTTTGAGCGTAACCTAATAGGTGATTGGTACCGGAGAAAGTGATGGGGGGGTTAAATAAGGGAATGCAGGAGTTTTGCGTCAGACGATATATGTTTATGTTCGAGTTTTtgt                                                                            <  2:30861/143‑1 (MQ=255)
                                                                         aTGCCTAAATGATTGGCAATAGGCTATGCCTAATGTTTTGAGCGAAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTtgtg                                                                           <  2:148989/148‑1 (MQ=255)
                                                                         aTGACTAATTGATTGTAAATAGGCTATGCCTAATGTTTTGAGCGTAAACTAATAGTTGATGGCTACATCAGAATGTGATGTGTGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGATTAAGTTCCAGTTTTtgtta                                                                          <  6:309920/149‑3 (MQ=255)
                                                                             cTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGCGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGtt                                                                      >  5:28522/1‑149 (MQ=255)
                                                                             cTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGCGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGtt                                                                      >  5:23545/1‑149 (MQ=255)
                                                                             cTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGAAGGCGACAGCAGATAGTGATGACGGGGTTAAAAAAAGTAATCCAGGAGTTTTCCGTCACACCAAATAAGTTTAAGTACCAGTTTTTGTGagaac                                                                      >  1:30861/1‑145 (MQ=255)
                                                                               aaTTGATTGTCCATAGCCTATGCCTAATGATTTGACCCTCACCTACTCCGCCACCCCGACAGCAGCAAGTGATGGGCGCGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCt                                                                    <  2:275213/149‑1 (MQ=255)
                                                                               aaTTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCt                                                                    >  6:186001/1‑149 (MQ=255)
                                                                                 ttgattgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTg                                                                   >  3:93103/1‑148 (MQ=255)
                                                                                     ttgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCttt                                                              >  3:116945/1‑149 (MQ=255)
                                                                                        tCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTcc                                                           >  7:128903/1‑149 (MQ=255)
                                                                                        tCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTcc                                                           >  1:137337/1‑149 (MQ=255)
                                                                                        gccaTAGCCTATGCCGAATGTGTACAGCCTCACCTAATACGCGACGCCGCCCGCAGAACGTGACGGGGGGGCTAAATAACGGAATCCACGAGGTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTcc                                                           <  6:113726/146‑1 (MQ=255)
                                                                                          aaTAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGt                                                         >  4:222608/1‑149 (MQ=255)
                                                                                          aaTAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGt                                                         >  5:138179/1‑149 (MQ=255)
                                                                                          aaTAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGt                                                         >  5:118303/1‑149 (MQ=255)
                                                                                           aTAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagtttaag                                                                                         <  2:244868/116‑1 (MQ=255)
                                                                                              ggCTATGCCATATGTATTGCGCGTAACCACATAGGTGCTGGCGCCAGCAGAAAGGGACGGTGCGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGTCCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCa                                                      <  8:166042/148‑1 (MQ=255)
                                                                                               gCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGtt                                                        >  5:260353/1‑145 (MQ=255)
                                                                                               ccgaTGCCTAATGTGTTGCGCGTACCCTACTAGCTCATGGCGACAGCACAAAGTGATGGGGGGGCTAAATAACGGAATCCAGGAGTTTTCCGGCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGa                                                    <  7:28021/146‑1 (MQ=255)
                                                                                                   tGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTc                                                 >  7:238697/1‑148 (MQ=255)
                                                                                                    gCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCa                                                >  4:1565/1‑148 (MQ=255)
                                                                                                     ccgactGTTTGGAGCGTACCCTAATCGGGGATGGCGACAGCAGAAACTGATGCGGGGGGTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCaa                                               <  3:323106/143‑1 (MQ=255)
                                                                                                      cTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGtt                                                                      >  5:362252/1‑124 (MQ=255)
                                                                                                      cTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGtt                                                                      <  6:362252/124‑1 (MQ=255)
                                                                                                       tAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCGAGCAGTTTTCCGTCAGACAAAATAAGTTTAAGTTACAGTTTTTGTGATGTACTGGCTTTTCCGTTCAGATTCaa                                               >  6:103164/1‑146 (MQ=255)
                                                                                                       tAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAagag                                            >  3:141756/1‑149 (MQ=255)
                                                                                                       tAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAagag                                            >  6:272844/1‑149 (MQ=255)
                                                                                                       tAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAagag                                            >  6:344001/1‑149 (MQ=255)
                                                                                                        aaTGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGc                                           >  1:241912/1‑149 (MQ=255)
                                                                                                        aaTGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGATAAAGTACCATTATTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGc                                           >  2:135253/1‑149 (MQ=255)
                                                                                                         aTGTTTTGCCCGTCACCTAATCCGTGATGGCGACTGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCATGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAagag                                            <  2:40578/147‑1 (MQ=255)
                                                                                                           gatttGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACTGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGatttttt                                                                            >  6:106778/3‑111 (MQ=255)
                                                                                                           gTTTTGAGCGTAACCTAATAGGTGATGGCGACTTCAGAAAGTGATGGTGGTGTTAAATAAGTGAATCCTGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTtgt                                                                            <  5:106778/113‑1 (MQ=255)
                                                                                                           gTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACTGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCttt                                        >  1:318962/1‑149 (MQ=255)
                                                                                                            ttttGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTc                                       <  2:241912/149‑1 (MQ=255)
                                                                                                            ttttGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGCGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCttt                                        <  6:28522/148‑1 (MQ=255)
                                                                                                            ttttGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGCGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCttt                                        <  6:23545/148‑1 (MQ=255)
                                                                                                             tttGAGCGTAACCTAATAGGCGATGGCGACAGCAGAAAGTCATGGTGGGGCTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTc                                       <  4:109414/148‑1 (MQ=255)
                                                                                                              ttGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCttt                                        >  1:106208/1‑146 (MQ=255)
                                                                                                                gAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGa                                    <  5:272844/148‑1 (MQ=255)
                                                                                                                  gCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATAc                                 >  8:39005/1‑149 (MQ=255)
                                                                                                                   cGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTGCTGGCTTTTCCTTTCAGATTCAAGAGCTTTCAgccagc                                >  6:353058/1‑144 (MQ=255)
                                                                                                                     tAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCt                                                                    >  4:187220/1‑111 (MQ=255)
                                                                                                                     tAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCt                                                                    <  3:187220/111‑1 (MQ=255)
                                                                                                                     tAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTa                              >  6:313749/1‑149 (MQ=255)
                                                                                                                     tAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTa                              <  8:238697/149‑1 (MQ=255)
                                                                                                                          tAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACCGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTAAGATTCAAGAGCTTTCAGATACTTACCCAc                         >  7:50908/1‑149 (MQ=255)
                                                                                                                           aaTAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAATTTTAAGTTCCAGTTTTTGTGATGTTATGGCTTTTCCGTTCAGATTCAATAGCTTTCAGATACTTACCCACt                        >  5:302943/1‑149 (MQ=255)
                                                                                                                            aTAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACtt                       <  5:186001/149‑1 (MQ=255)
                                                                                                                             tAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTTGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACttt                      >  1:83239/1‑149 (MQ=255)
                                                                                                                               ggTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCa                    >  3:315908/1‑149 (MQ=255)
                                                                                                                                          cagcagAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCgct         <  6:260353/149‑1 (MQ=255)
                                                                                                                                              agAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCGCTGCTa     <  8:128903/149‑1 (MQ=255)
                                                                                                                                                 aaGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTTGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCGCTGCTATGt  <  2:83239/149‑1 (MQ=255)
                                                                                                                                                 aaGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCGCTGCTATGt  <  5:344001/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCGCTGCTATGT  >  NC_000913/1419563‑1419856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: