| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,698,695 | A→G | 6.7% | V162V (GTT→GTC) | malI ← | transcriptional repressor of Mal regulon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,698,695 | 0 | A | G | 6.7% | 180.4 / 3.2 | 60 | V162V (GTT→GTC) | malI | transcriptional repressor of Mal regulon |
| Reads supporting (aligned to +/- strand): ref base A (23/33); new base G (2/2); total (25/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
ATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACAG > NC_000913/1698550‑1698838 | aTTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAAcc < 4:15904/149‑1 (MQ=255) gtagAGTTGCACAATCGCCCCCAACCCGTACTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATc < 8:94115/148‑1 (MQ=255) gtagAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATc < 3:231773/148‑1 (MQ=255) agagTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATc < 5:89905/146‑1 (MQ=255) agagTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATc > 6:89905/1‑146 (MQ=255) gagTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAc < 7:117541/148‑1 (MQ=255) gagTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAc < 1:149203/148‑1 (MQ=255) gTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTa > 6:284518/1‑141 (MQ=255) gTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTa < 5:284518/141‑1 (MQ=255) gTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcatc < 7:204605/149‑1 (MQ=255) gTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcat > 3:291422/1‑148 (MQ=255) tcattaaCCCGTGCAGAACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCCTTGCGAATGAGATGCTCCGTCCACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAGATCTTCAAGATTAcg > 6:109499/6‑148 (MQ=255) cccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTgg < 3:174041/149‑1 (MQ=255) ccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTgg > 4:164811/1‑148 (MQ=255) ccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTgg > 3:199128/1‑148 (MQ=255) aaCCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACACCTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCAc > 1:329210/1‑149 (MQ=255) gCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGa > 4:260365/1‑148 (MQ=255) tGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGa > 1:180632/1‑149 (MQ=255) aGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATa < 6:10880/149‑1 (MQ=255) aGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTCGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGAtc < 5:249769/149‑2 (MQ=255) aGGAACTTTGCCCTCCCAGCCAGGCGATCAGCTGATGCACATTGCGAATGAGATTCTCCGTCAACACCTGTGCCGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGCGa > 2:78220/1‑147 (MQ=255) aCTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCtt < 3:223842/149‑1 (MQ=255) cTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCttt < 5:26224/149‑1 (MQ=255) gCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTttc < 1:46279/148‑1 (MQ=255) ccTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTttcttc < 1:283928/149‑1 (MQ=255) cTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTtcttct < 4:304247/149‑1 (MQ=255) tCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTg > 5:116704/1‑149 (MQ=255) ccagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGc < 5:304331/148‑1 (MQ=255) ccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATcc > 2:303847/1‑149 (MQ=255) ccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATcc < 5:249316/149‑1 (MQ=255) aTCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGTACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAg < 5:109499/149‑1 (MQ=255) aTGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATc < 6:116704/147‑1 (MQ=255) ccATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGGACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACt > 7:342722/1‑149 (MQ=255) ccATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGGACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACt > 7:347621/1‑149 (MQ=255) ccATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACt > 6:168096/1‑149 (MQ=255) aTTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTc < 2:329210/149‑1 (MQ=255) gCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATCACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCt < 1:200889/148‑1 (MQ=255) aaTGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAg > 8:322753/1‑129 (MQ=255) aaTGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAg < 7:322753/129‑1 (MQ=255) gaTGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCg > 6:357031/1‑149 (MQ=255) tCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGAt < 3:121442/61‑1 (MQ=255) tCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGAt > 4:121442/1‑61 (MQ=255) cGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAAtt < 3:164811/148‑1 (MQ=255) gTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGGACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTAc < 8:347621/149‑1 (MQ=255) gTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGGACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTAc < 8:342722/149‑1 (MQ=255) gTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTAc < 3:260365/149‑1 (MQ=255) aacaacTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACca > 6:203180/1‑148 (MQ=255) aacaacTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACca > 6:208302/1‑148 (MQ=255) caacTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCacac > 4:185433/1‑149 (MQ=255) aacTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCaa < 2:358494/139‑1 (MQ=255) aacTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCaa > 1:358494/1‑139 (MQ=255) tgCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATc < 3:185433/147‑1 (MQ=255) cAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGaca < 4:291422/149‑1 (MQ=255) gCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAAtt > 4:209742/1‑132 (MQ=255) gCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAAtt < 3:209742/132‑1 (MQ=255) gCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACAcc < 5:168096/149‑1 (MQ=255) aTGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGAtt < 4:199128/149‑1 (MQ=255) tgcGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAATTCACCGGGATAGCTTTTTCTTCTGCCATCAGTCGCACTTCATCGCTACTTCCTGCAGAGCCGGCAATTACCAAACCATCTACACCCTGCTTCAGTa > 3:42102/3‑148 (MQ=255) cGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACa < 1:303847/149‑1 (MQ=255) gggCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACAg > 8:157096/1‑149 (MQ=255) | ATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAAAATCACCGGGATAGCTTTTTCTTCTGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACAG > NC_000913/1698550‑1698838 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |