Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,806,380 T→C 5.9% I4T (ATC→ACC)  pfkB → 6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,806,3800TC 5.9% 206.9 / 3.2 68I4T (ATC→ACC) pfkB6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base T (36/28);  new base C (2/2);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

GCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGG  >  NC_000913/1806232‑1806527
                                                                                                                                                    |                                                                                                                                                   
gCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTAt                                                                                                                                                     <  6:17648/149‑1 (MQ=255)
       gCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTATCCGCTGATTCTGTGCCAGACTTAAATCAGCCTATAGGACGAAATGAAGGTACGAATCTATACg                                                                                                                                              >  1:246694/1‑149 (MQ=255)
         gAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATATATACGtt                                                                                                                                            <  4:305245/149‑1 (MQ=255)
            aaCAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                                                                                          >  5:248559/1‑148 (MQ=255)
                 aTTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACt                                                                                                                                      >  8:176183/1‑147 (MQ=255)
                 aTTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACt                                                                                                                                      <  7:176183/147‑1 (MQ=255)
                  tttttATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCTGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgc                                                                                                                                   <  6:229015/149‑1 (MQ=255)
                         ttatATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCAGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATCGTACGTATCTATACGTTGACACTTGCGACctc                                                                                                                             >  2:284607/1‑148 (MQ=255)
                             atatatTTAGCTGCAATATTTTAATTAAAGCTCCTATTAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                                                                                         >  6:28738/1‑148 (MQ=255)
                              tatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                                                                                       >  1:149080/1‑149 (MQ=255)
                              tatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                                                                                       >  8:60963/1‑149 (MQ=255)
                                 atTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAg                                                                                                                    >  7:68009/1‑149 (MQ=255)
                                      tCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaa                                                                                                               >  1:327746/1‑149 (MQ=255)
                                          cAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAAt                                                                                                           >  6:133247/1‑149 (MQ=255)
                                             aaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAc                                                                                                        >  3:329555/1‑149 (MQ=255)
                                                 ttAAATAAAGCTCCAATTAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTTAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcccc                                                                                                     <  6:69013/148‑1 (MQ=255)
                                                    aataaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTGTAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCa                                                                                                  >  6:205959/1‑148 (MQ=255)
                                                      taaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAt                                                                                               >  8:150752/1‑149 (MQ=255)
                                                       aaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGTCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaac                                                                                                              >  3:78726/1‑133 (MQ=255)
                                                       aaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGTCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaac                                                                                                              <  4:78726/133‑1 (MQ=255)
                                                          gCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTAt                                                                                           <  7:60963/149‑1 (MQ=255)
                                                            tCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCGTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATcc                                                                                         >  4:287899/1‑149 (MQ=255)
                                                            tCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATcc                                                                                         >  4:292746/1‑149 (MQ=255)
                                                             ccAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATccc                                                                                        >  5:134374/1‑149 (MQ=255)
                                                               aaTAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCg                                                                                       >  6:65327/1‑148 (MQ=255)
                                                               aaTAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCg                                                                                       >  1:60065/1‑148 (MQ=255)
                                                                aTAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCg                                                                                       >  3:165905/1‑147 (MQ=255)
                                                                aTAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa                                                                                     <  4:329555/149‑1 (MQ=255)
                                                                   aaTCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAgg                                                                                   <  7:144716/148‑1 (MQ=255)
                                                                     tCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGaaa                                                                                <  4:332733/149‑1 (MQ=255)
                                                                       atatTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTACCTATACGtt                                                                                                                                            <  6:210107/87‑1 (MQ=255)
                                                                       atatTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTACCTATACGtt                                                                                                                                            >  5:210107/1‑87 (MQ=255)
                                                                         atTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTg                                                                            >  4:157300/1‑149 (MQ=255)
                                                                          atGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgc                                                                           >  6:311118/2‑149 (MQ=255)
                                                                           tGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcg                                                                          >  3:84786/1‑149 (MQ=255)
                                                                            gTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcgc                                                                         >  1:302590/1‑149 (MQ=255)
                                                                            gTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGAATGAAATCAGCCTATAGGATTAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATTGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcg                                                                          <  1:284607/148‑1 (MQ=255)
                                                                               aaTTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTAGAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCACCAATTACCCCGCAAATTTATCCCGAAGGAATACTGCGCTg                                                                       >  6:239014/1‑148 (MQ=255)
                                                                                aTTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTa                                                                     <  5:133247/149‑1 (MQ=255)
                                                                                        aCTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTa                                                                                            <  5:44569/118‑1 (MQ=255)
                                                                                        aCTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTa                                                                                            >  6:44569/1‑118 (MQ=255)
                                                                                        aCTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCg                                                             <  8:162293/149‑1 (MQ=255)
                                                                                              cGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGtt                                                        <  5:65327/148‑1 (MQ=255)
                                                                                               gCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATAc                                                                                                                                               <  6:103208/60‑1 (MQ=255)
                                                                                               gCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATAc                                                                                                                                               >  5:103208/1‑60 (MQ=255)
                                                                                                   aTTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAAc                                                   <  5:311118/148‑1 (MQ=255)
                                                                                                   aTTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAAc                                                   <  2:327746/148‑1 (MQ=255)
                                                                                                       ggTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCgg                                               <  2:302590/148‑1 (MQ=255)
                                                                                                        gTGCCAGACTGAAATCAGCCTGTAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCgggg                                             <  5:205959/149‑1 (MQ=255)
                                                                                                              gACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGggcggcg                                       >  2:221301/1‑149 (MQ=255)
                                                                                                               aCTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGggcggcgg                                      <  6:223389/149‑1 (MQ=255)
                                                                                                                 tGAAATCAGCCTATAGGAGGAAATGATGGTACGTACCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcg                                                                          <  5:315086/111‑1 (MQ=255)
                                                                                                                 tGAAATCAGCCTATAGGAGGAAATGATGGTACGTACCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcg                                                                          >  6:315086/1‑111 (MQ=255)
                                                                                                                  gAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCAt                                   >  6:243473/1‑149 (MQ=255)
                                                                                                                     aTCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCaa                                <  1:221301/149‑1 (MQ=255)
                                                                                                                      tCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAAc                               >  3:229076/1‑149 (MQ=255)
                                                                                                                         gCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAg                                                                                    <  7:342985/93‑1 (MQ=255)
                                                                                                                         gCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAg                                                                                    >  8:342985/1‑93 (MQ=255)
                                                                                                                         gCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTc                            <  8:68009/149‑1 (MQ=255)
                                                                                                                                ggaggaAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCcgc                      <  7:150752/148‑1 (MQ=255)
                                                                                                                                 gaggaAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCgcgc                    >  8:269989/1‑149 (MQ=255)
                                                                                                                                     aaaTGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCAtt                <  2:149080/149‑1 (MQ=255)
                                                                                                                                     aaaTGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCAtt                >  8:88983/1‑149 (MQ=255)
                                                                                                                                      aaTGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTg               >  4:135857/1‑149 (MQ=255)
                                                                                                                                       atgatgGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCAt                 >  2:290813/1‑146 (MQ=255)
                                                                                                                                       atgatgGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCAt                 <  1:290813/146‑1 (MQ=255)
                                                                                                                                       atgatgGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGc              <  4:229076/149‑1 (MQ=255)
                                                                                                                                          atgGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCa           >  5:111672/1‑149 (MQ=255)
                                                                                                                                          atgGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCa           <  7:88983/149‑1 (MQ=255)
                                                                                                                                                    tCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAgg  <  6:117599/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGG  >  NC_000913/1806232‑1806527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: