Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,860,504 C→A 6.6% G277* (GGA→TGA)  ydjL ← putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,860,5040CA 6.6% 223.8 / 4.0 69G277* (GGA→TGA) ydjLputative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (27/37);  new base A (3/2);  total (30/39)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

ATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAGCGCGCCACCACATCTTCGGTAGAACCATT  >  NC_000913/1860359‑1860651
                                                                                                                                                 |                                                                                                                                                   
atcGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAAc                                                                                                                                                  <  5:33056/149‑1 (MQ=255)
   ggTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAAcgc                                                                                                                                                <  6:262515/148‑1 (MQ=255)
     tttGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTa                                                                                                                                             >  6:320329/1‑149 (MQ=255)
     tttGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTa                                                                                                                                             <  1:269214/149‑1 (MQ=255)
       tGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTAc                                                                                                                                            <  2:278890/148‑1 (MQ=255)
         aCTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCAc                                                                                                                                         >  7:287899/1‑149 (MQ=255)
           tttGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACtt                                                                                                                                       >  6:131258/1‑149 (MQ=255)
                     ccgcTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCgg                                                                                                                             <  1:351659/149‑1 (MQ=255)
                         tGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCg                                                                                                                          >  7:109846/1‑148 (MQ=255)
                           gCTAATAGCCTGATAGCGTTACGCCAAGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAg                                                                                                                       >  5:82527/1‑149 (MQ=255)
                            cTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAg                                                                                                                       >  5:24457/1‑148 (MQ=255)
                                tAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCAttt                                                                                                                  <  5:320329/149‑1 (MQ=255)
                                tAGCCTGATAGCGTTACGCCATGAGGTGGAGGCATAGGCCATATGCCCAATGATGCTGTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCTAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCAttt                                                                                                                  <  5:33989/149‑1 (MQ=255)
                                    cTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGAt                                                                                                              >  8:310306/1‑149 (MQ=255)
                                       aTAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACACGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGc                                                                                                           <  3:53269/149‑1 (MQ=255)
                                             ttACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCa                                                                                                                     >  6:354144/1‑133 (MQ=255)
                                             ttACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCa                                                                                                                     <  5:354144/133‑1 (MQ=255)
                                                cGCCATGAGGGGGAGTCAAAGGCCATATGACCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCa                                                                                                   <  6:256575/148‑1 (MQ=255)
                                                 gCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGt                                                                                                 <  6:16531/149‑1 (MQ=255)
                                                    aTGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGc                                                                                               >  8:66717/1‑148 (MQ=255)
                                                     tGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGa                                                                                             <  5:257262/149‑1 (MQ=255)
                                                             aGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCa                                                                                     <  7:66717/149‑1 (MQ=255)
                                                                cATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTcccc                                                                                                                                   >  7:158110/1‑100 (MQ=255)
                                                                cATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTcccc                                                                                                                                   <  8:158110/100‑1 (MQ=255)
                                                                 aTAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCAAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCg                                                                                  >  3:30048/1‑148 (MQ=255)
                                                                 aTAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCAAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCg                                                                                  >  1:213595/1‑148 (MQ=255)
                                                                  tAGGCCATATTCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGa                                                                                <  5:148643/149‑1 (MQ=255)
                                                                  tAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCg                                                                                  >  5:318190/1‑147 (MQ=255)
                                                                  tAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGa                                                                                <  7:119929/149‑1 (MQ=255)
                                                                  tAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGa                                                                                <  8:287899/149‑1 (MQ=255)
                                                                  tAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGa                                                                                <  2:205863/149‑1 (MQ=255)
                                                                     gCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCa                                                                             >  1:211964/1‑149 (MQ=255)
                                                                     gCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCa                                                                             >  8:193455/1‑149 (MQ=255)
                                                                       cATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCAtt                                                                           <  6:82527/149‑1 (MQ=255)
                                                                       cATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCAtt                                                                           <  2:211964/149‑1 (MQ=255)
                                                                        atatGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCAtt                                                                           >  1:76846/1‑148 (MQ=255)
                                                                         tatGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCa                                                                         <  7:77759/149‑1 (MQ=255)
                                                                                aaTGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCAc                                                                    >  1:125840/1‑147 (MQ=255)
                                                                                  tgatgCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCa                                                                                                   <  6:55753/114‑1 (MQ=255)
                                                                                  tgatgCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCa                                                                                                   >  5:55753/1‑114 (MQ=255)
                                                                                  tgatgCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGt                                                                >  3:150723/1‑149 (MQ=255)
                                                                                   gatgCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTc                                                               >  7:303919/1‑149 (MQ=255)
                                                                                    atgCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCCTTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTc                                                               <  8:109846/148‑1 (MQ=255)
                                                                                               ccAGGCGCTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCAGTCAAACGCGTACCACTTCCCCGTTCGGGCGCAGCATTTCGCTGGCTTGTTTCAGTGAGATATTGGCACCGGAGCATACAATCACCAGACCCAGATTGACtt                                                   >  8:76045/1‑149 (MQ=255)
                                                                                                cAGGCGGTAAGGTCATTAATCGAGAAATCAAGAGGATTGAAGCCGATTACAACGCGTACCACTTCCCCGTGCGGGCGGAGCATATCGATGGCTTGTTTCAGTGCGATATTGCCACCGGAGCATTCAATCACCAGTCCCAGATTGTCttt                                                  <  5:251494/149‑1 (MQ=255)
                                                                                                 aGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTAGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTg                                                 >  4:32220/1‑149 (MQ=255)
                                                                                                      gTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCa                                                                  <  1:46707/127‑1 (MQ=255)
                                                                                                      gTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCa                                                                  >  2:46707/1‑127 (MQ=255)
                                                                                                         aTGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAAt                                         >  6:344821/1‑149 (MQ=255)
                                                                                                            tCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCtt                                                   <  3:132284/136‑1 (MQ=255)
                                                                                                            tCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCtt                                                   >  4:132284/1‑136 (MQ=255)
                                                                                                              aTTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCt                                    <  3:32220/149‑1 (MQ=255)
                                                                                                              aTTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCt                                    >  7:365598/1‑149 (MQ=255)
                                                                                                               ttAATCGAGAAATCAAGAGGTTTGAAGCCCATTCAAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTg                                   <  2:213595/149‑1 (MQ=255)
                                                                                                               ttAATCGAGAAATCAAGAGGTTTGAAGCCCATTCAAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTg                                   <  4:30048/149‑1 (MQ=255)
                                                                                                                 aaTCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTgg                                  <  7:314247/148‑1 (MQ=255)
                                                                                                                  aTCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCa                                <  8:303919/149‑1 (MQ=255)
                                                                                                                   tCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAAt                                                                       >  4:28297/1‑109 (MQ=255)
                                                                                                                   tCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAAt                                                                       <  3:28297/109‑1 (MQ=255)
                                                                                                                   tCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAg                               >  1:234976/1‑149 (MQ=255)
                                                                                                                   tCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAg                               <  7:193455/149‑1 (MQ=255)
                                                                                                                     gagaAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAgc                              <  6:318190/148‑1 (MQ=255)
                                                                                                                             aaGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAGCGCGccacca                     <  7:310306/149‑1 (MQ=255)
                                                                                                                              agagGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAGCGCGCcaccac                    <  2:234976/149‑1 (MQ=255)
                                                                                                                                  gTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAGCGCGCCACCACATCt                >  5:143666/1‑149 (MQ=255)
                                                                                                                                      gAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAGCGCGCCACCACATCTTCg             <  4:150723/148‑1 (MQ=255)
                                                                                                                                         gCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAGCGCGCCACCACATCTTCGGTAg         <  5:344821/149‑1 (MQ=255)
                                                                                                                                         gCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAGCGCGCCACCACATCTTCGGTAg         <  6:143666/149‑1 (MQ=255)
                                                                                                                                            cATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAGCGCGCCACCACATCTTCGGTAGAAc      >  2:313379/1‑149 (MQ=255)
                                                                                                                                                 cAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAGCGCGCCACCACATCTTCGGTAGAACCAtt  >  7:180657/1‑148 (MQ=255)
                                                                                                                                                 cAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAGCGCGCCACCACATCTTCGGTAGAACCAtt  >  7:175717/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTTGTTCCAGGCGGTAATGTCATTAATCGAGAAATCAAGAGGTTTGAAGCCCATTCCAACGCGTACCACTTCCCCGTTCGGGCGGAGCATTTCGATGGCTTGTTTCAGTGCGATATTGGCACCGGAGCATTCAATCACCAGTCCCAGATTGTCTTTGCCACAAATTTGCTGGCAGCGCGCCACCACATCTTCGGTAGAACCATT  >  NC_000913/1860359‑1860651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: