Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,433,237 C→T 5.5% P230P (CCG→CCA)  accD ← acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,433,2370CT 5.5% 228.5 / 2.6 72P230P (CCG→CCA) accDacetyl‑CoA carboxylase, beta (carboxyltransferase) subunit
Reads supporting (aligned to +/- strand):  ref base C (31/37);  new base T (2/2);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

GATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCA  >  NC_000913/2433089‑2433384
                                                                                                                                                    |                                                                                                                                                   
gATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGATGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCTAACGGTCTGTTCGATAACAcgc                                                                                                                                                     <  5:220886/149‑1 (MQ=255)
     atcaACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGAcc                                                                                                                                                >  1:352491/1‑149 (MQ=255)
     atcaACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGACTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGAcc                                                                                                                                                >  3:251743/1‑149 (MQ=255)
     atcaACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATATCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGAcc                                                                                                                                                >  6:231488/1‑149 (MQ=255)
      tcaACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGGCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCg                                                                                                                                               <  2:89172/149‑1 (MQ=255)
           ttCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCa                                                                                                                                            >  5:278097/1‑147 (MQ=255)
                 aGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGAt                                                                                                                                    <  5:231488/149‑1 (MQ=255)
                 aGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGAt                                                                                                                                    <  3:237225/149‑1 (MQ=255)
                        tCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCCGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACCCGCGGACCGGCAAAGCCGATTAACGCt                                                                                                                             >  6:27408/1‑149 (MQ=255)
                        tCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCt                                                                                                                             >  6:226263/1‑149 (MQ=255)
                        tCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCt                                                                                                                             >  2:331030/1‑149 (MQ=255)
                          gCCAGTTTCAGGCGCATTTCCGGATGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCttt                                                                                                                           >  8:318124/1‑149 (MQ=255)
                          gCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCttt                                                                                                                           <  2:193990/149‑1 (MQ=255)
                          gCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCttt                                                                                                                           >  5:139046/1‑149 (MQ=255)
                          gCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCtt                                                                                                                            <  7:76904/148‑1 (MQ=255)
                          gCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCtt                                                                                                                            <  6:278097/148‑1 (MQ=255)
                            cAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACAcgc                                                                                                                                                     >  5:51602/1‑121 (MQ=255)
                            cAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACAcgc                                                                                                                                                     <  6:51602/121‑1 (MQ=255)
                            cAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCg                                                                                                                         <  6:139046/149‑1 (MQ=255)
                                    ggCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGc                                                                                                                  >  7:60829/1‑148 (MQ=255)
                                       gcATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATg                                                                                                              >  5:17275/1‑149 (MQ=255)
                                          tttCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGa                                                                                                                <  6:250815/144‑1 (MQ=255)
                                          tttCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGa                                                                                                                >  5:250815/1‑144 (MQ=255)
                                              cggacgacggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTgaga                                                                                                        <  7:277369/148‑1 (MQ=255)
                                              cggacgacggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGAt                                                                                                       <  6:53865/149‑1 (MQ=255)
                                               ggacgacggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATc                                                                                                      <  2:140780/149‑1 (MQ=255)
                                                    acggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGcc                                                                                                   >  3:72343/1‑147 (MQ=255)
                                                    acggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCa                                                                                                 <  6:43303/149‑1 (MQ=255)
                                                      ggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAg                                                                                                >  6:230080/1‑148 (MQ=255)
                                                       gacgaTCATGTCGATCGCGCCTTTCTCGAGCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCa                                                                                              <  1:228222/149‑1 (MQ=255)
                                                        acgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCAt                                                                                             >  2:221503/1‑149 (MQ=255)
                                                         cgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATg                                                                                            <  5:226263/149‑1 (MQ=255)
                                                          gaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATgg                                                                                           <  8:108546/149‑1 (MQ=255)
                                                           aTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGc                                                                                          <  3:142242/149‑1 (MQ=255)
                                                            tCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGtt                                                                                                            <  4:238548/130‑1 (MQ=255)
                                                            tCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGtt                                                                                                            >  3:238548/1‑130 (MQ=255)
                                                            tCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTATCGGTTAAGCGATGTTGAGATCGCCCAGCATGGc                                                                                          >  3:55006/1‑148 (MQ=255)
                                                                 tCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAAc                                                                                     <  4:251743/148‑1 (MQ=255)
                                                                  cGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCAAGGCGAAACtt                                                                                   >  4:63566/1‑149 (MQ=255)
                                                                   gATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGTGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTg                                                                                  >  6:11169/1‑149 (MQ=255)
                                                                    aTCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCGCAGCATGGCGAAACTTGc                                                                                 <  1:331030/149‑1 (MQ=255)
                                                                    aTCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGc                                                                                 <  5:230080/149‑1 (MQ=255)
                                                                     tCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGTGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCa                                                                                <  5:11169/149‑1 (MQ=255)
                                                                     tCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCa                                                                                <  8:307708/149‑1 (MQ=255)
                                                                           cTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGTGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATgg                                                                                           <  2:75593/132‑1 (MQ=255)
                                                                           cTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGTGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATgg                                                                                           >  1:75593/1‑132 (MQ=255)
                                                                           cTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAaca                                                                          >  6:13492/1‑149 (MQ=255)
                                                                             ttCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCa                                                                                <  7:82538/141‑1 (MQ=255)
                                                                             ttCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCa                                                                                >  8:82538/1‑141 (MQ=255)
                                                                              tctcGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACAcc                                                                        <  1:221503/148‑1 (MQ=255)
                                                                                tcGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGcc                                                                     >  7:259904/1‑149 (MQ=255)
                                                                                  gATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCa                                                                   >  7:178536/1‑149 (MQ=255)
                                                                                        gAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCAtcgtcg                                                             <  6:17275/149‑1 (MQ=255)
                                                                                        gAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCAtcgtcg                                                             <  8:178536/149‑1 (MQ=255)
                                                                                              aCTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGTTATGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTAAGCGATGTTGAGATCGGCGAGCATTGCGAAACTTGCAGAAACACCGCCCATCgtcgggtcgg                                                       <  1:32443/149‑1 (MQ=255)
                                                                                                tGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGggtcggt                                                      >  1:117683/1‑148 (MQ=255)
                                                                                                tGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGggtcggt                                                      >  7:106973/1‑148 (MQ=255)
                                                                                                        aaTCCAGGCGGCAGTTTTTCGCTAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAATCTTGCAGAAACACCGCCCATCGTCGGGGCGGTCAGCACGga                                             <  5:71929/149‑1 (MQ=255)
                                                                                                         aTCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGgag                                            <  8:60829/149‑1 (MQ=255)
                                                                                                         aTCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGgag                                            <  2:117683/149‑1 (MQ=255)
                                                                                                          tCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACg                                               >  4:89829/1‑145 (MQ=255)
                                                                                                          tCCAGGCGGCAGTTTTTCGCGAACGGGCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGGCGGTCAGCACGgaga                                           <  4:45677/149‑1 (MQ=255)
                                                                                                             aGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTATCGGTTCAGCGATGTTCAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATg                                         >  2:263284/1‑148 (MQ=255)
                                                                                                                   cAGTTTTTCGAGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGAGGTTCAGATCGCCCAGCATGGCCAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCCGCACGGCGATGTACGGc                                   >  6:119356/1‑148 (MQ=255)
                                                                                                                    aGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCaa                                 <  8:259904/149‑1 (MQ=255)
                                                                                                                      tttttCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTAAGCACGGAGATGTACGGCAAGc                               >  5:203701/1‑149 (MQ=255)
                                                                                                                        tttCGCGAACGGTCTGTTCGATAACACGCGGACCTGCAAAGCCGATTTACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGACACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGcc                              <  2:352491/148‑1 (MQ=255)
                                                                                                                                      tGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCAtttt               >  2:300440/1‑149 (MQ=255)
                                                                                                                                      tGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCAtttt               >  7:91831/1‑149 (MQ=255)
                                                                                                                                        ttCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCAttttt              <  8:106973/148‑1 (MQ=255)
                                                                                                                                        ttCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCAttttt              <  5:13492/148‑1 (MQ=255)
                                                                                                                                            aTAACACGCTGACCGGCTAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGTGTTCGCCCAGCATGGCGAAACTTTCAGAAACACCGCCCATCGTCGGGTAGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCGAg         <  4:55006/149‑1 (MQ=255)
                                                                                                                                               acacGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGc      <  3:63566/149‑1 (MQ=255)
                                                                                                                                                cacGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGcc     >  3:78455/1‑149 (MQ=255)
                                                                                                                                                 acGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCg    <  4:72343/149‑1 (MQ=255)
                                                                                                                                                   gcgGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCg    >  5:7551/1‑147 (MQ=255)
                                                                                                                                                   gcgGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCg    >  7:218508/1‑147 (MQ=255)
                                                                                                                                                   gcgGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCa  <  8:218508/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCA  >  NC_000913/2433089‑2433384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑