Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,330,3961.T22.2% 83.3 / 32.9 36intergenic (+15/+21)sohB/yciNinner membrane protein, S49 peptidase family protein/DUF2498 protein YciN
Reads supporting (aligned to +/- strand):  ref base . (13/15);  new base T (4/4);  total (17/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCA  >  NC_000913/1330248‑1330541
                                                                                                                                                     |                                                                                                                                                 
aaGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAgg                                                                                                                                                    >  7:62797/1‑149 (MQ=255)
    ccGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTaa                                                                                                                                               <  5:51438/149‑1 (MQ=255)
      gTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGa                                                                                                                                             >  5:15947/1‑149 (MQ=255)
                cAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGttt                                                                                                                                   <  7:100691/149‑1 (MQ=255)
                 aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGttt                                                                                                                                   <  2:22521/148‑1 (MQ=255)
                       cGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTaa                                                                                                                                               >  1:120973/1‑130 (MQ=255)
                       cGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTaa                                                                                                                                               <  2:120973/130‑1 (MQ=255)
                        gCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTaa                                                                                                                           <  1:64214/149‑1 (MQ=255)
                                      gaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGata                                                                                                             >  4:19061/2‑149 (MQ=255)
                                      aaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGtttgtc                                                                                                                                >  7:88243/1‑127 (MQ=255)
                                      aaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGtttgtc                                                                                                                                <  8:88243/130‑4 (MQ=255)
                                            cTCATTGACCGATTCTCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTc                                                                                                       <  6:15947/149‑1 (MQ=255)
                                            cTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTc                                                                                                       <  8:62797/149‑1 (MQ=255)
                                               aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt                                                                                                      >  5:82153/1‑147 (MQ=255)
                                               aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt                                                                                                      <  6:82153/147‑1 (MQ=255)
                                                   aCCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAg                                                                                                 <  8:55828/148‑1 (MQ=255)
                                                   aCCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAg                                                                                                 >  7:55828/1‑148 (MQ=255)
                                                            ccGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGtttg                                                                                                                                  <  6:28081/106‑2 (MQ=39)
                                                            ccGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGtttg                                                                                                                                  >  5:28081/1‑105 (MQ=39)
                                                                                gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAgat                                                                                                                 <  5:57421/103‑1 (MQ=255)
                                                                                gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAgat                                                                                                                 >  6:57421/1‑103 (MQ=255)
                                                                                                        tggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTgg                                           <  3:19061/149‑1 (MQ=255)
                                                                                                            aGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAAt                                       <  8:25150/149‑1 (MQ=255)
                                                                                                                     aaaaGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAAc                                                                                                                      <  2:57945/61‑1 (MQ=255)
                                                                                                                     aaaaGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAAc                                                                                                                      >  1:57945/1‑61 (MQ=255)
                                                                                                                           cATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaa                         >  8:9770/1‑148 (MQ=255)
                                                                                                                                   tAAAAGACAAACGCGAGGCTAAGCCCTCGCGTTTTGATTTAATCAACCAGATTATATTTTTCTGAAAGAACATGGGCCAGGTGTTTTAACATATTAAACACAGAGTTGATTTTGTCTGTTGGAAATACTTGATAATCTAAAAagtaga                 >  7:32524/1‑147 (MQ=255)
                                                                                                                                   tAAAAGACAAACGCGAGGCTAAGACCTAGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagtagt                 <  8:32524/148‑1 (MQ=255)
                                                                                                                                    aaaaGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg               >  1:84160/1‑149 (MQ=255)
                                                                                                                                       agcaaaaCGCGAGGTCTTAGCCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTg                                                   <  6:72052/106‑1 (MQ=38)
                                                                                                                                       agcaaaaCGCGAGGTCTTAGCCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTg                                                   >  5:72052/5‑110 (MQ=38)
                                                                                                                                       aGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCg            >  6:54991/1‑149 (MQ=255)
                                                                                                                                        gACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCgg           <  7:9770/149‑1 (MQ=255)
                                                                                                                                          aaaaCGCGAGGTCTTAGCCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGt          >  3:53257/2‑148 (MQ=255)
                                                                                                                                           aaaCGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                      >  6:125594/1‑135 (MQ=255)
                                                                                                                                           aaaCGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                      <  5:125594/135‑1 (MQ=255)
                                                                                                                                           aaaCGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa         >  5:48763/1‑148 (MQ=255)
                                                                                                                                                   ggCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCa  <  2:84160/147‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
AAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCA  >  NC_000913/1330248‑1330541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: