New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4530003 =42 (1.260)5 (0.160) 5/250 3.3 11.7% coding (241/1314 nt) sgcC putative PTS system EIIC permease component
?NC_000913 4530040 = 35 (1.090)coding (204/1314 nt) sgcC putative PTS system EIIC permease component
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGA  >  NC_000913/4529901‑4530188
                                                                                                                                           |                                                                                                                                                    
tCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTc                                                                                                                                              >  6:112780/1‑148 (MQ=255)
       ggTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGa                                                                                                                                                     <  6:25576/134‑1 (MQ=255)
       ggTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGa                                                                                                                                                     >  5:25576/1‑134 (MQ=255)
         tCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACt                                                                                                                                    >  1:120674/1‑149 (MQ=255)
                     ccAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGa                                                                                                                        >  1:163538/1‑149 (MQ=255)
                           tGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAgg                                                                                                                  <  2:120674/149‑1 (MQ=255)
                               gACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGt                                                                                                              >  2:8925/1‑149 (MQ=255)
                                     gagaAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATc                                                                                                         <  5:27603/148‑1 (MQ=255)
                                     gagaAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATc                                                                                                         <  1:8925/148‑1 (MQ=255)
                                        aagaaAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAACCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGcc                                                                                                      >  6:8345/1‑148 (MQ=255)
                                        aagaaAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCaa                                                                                                                                            >  2:25845/1‑110 (MQ=255)
                                        aagaaAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCa                                                                                                     >  1:88017/1‑149 (MQ=255)
                                        aagaaAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCa                                                                                                     >  3:58122/1‑149 (MQ=255)
                                          gaaAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATg                                                                                                   <  2:163538/149‑1 (MQ=255)
                                                              aTGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAc                                                                                <  3:5293/148‑1 (MQ=255)
                                                              aTGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAc                                                                                >  4:5293/1‑148 (MQ=255)
                                                               tGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCAc                                                                                           >  5:130320/1‑136 (MQ=255)
                                                               tGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCAc                                                                                           <  6:130320/136‑1 (MQ=255)
                                                                                  aTAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCAc                                                                                           >  2:11095/1‑117 (MQ=255)
                                                                                  aTAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCAc                                                                                           <  1:11095/117‑1 (MQ=255)
                                                                                      ccAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGa                                                                                               >  6:95751/1‑109 (MQ=255)
                                                                                      ccAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGa                                                                                               <  5:95751/109‑1 (MQ=255)
                                                                                        aaCGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATc                                                                                             >  5:39860/1‑109 (MQ=255)
                                                                                        aaCGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATc                                                                                             <  6:39860/109‑1 (MQ=255)
                                                                                                  gCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAAt                                           <  5:8345/149‑1 (MQ=255)
                                                                                                    aGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCg                                         >  1:43061/1‑149 (MQ=255)
                                                                                                               cAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAg                              >  3:27233/1‑149 (MQ=255)
                                                                                                                       aaGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCa                                                                                         <  3:86616/82‑1 (MQ=255)
                                                                                                                       aaGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCa                                                                                         >  4:86616/1‑82 (MQ=255)
                                                                                                                       aaGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCg                      <  2:88017/149‑1 (MQ=255)
                                                                                                                            aTGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTc                                                                                                             <  4:55726/57‑1 (MQ=255)
                                                                                                                            aTGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTc                                                                                                             >  3:55726/1‑57 (MQ=255)
                                                                                                                              gCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGAt                >  3:73395/1‑148 (MQ=255)
                                                                                                                                    cTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGc           <  8:10453/147‑1 (MQ=255)
                                                                                                                                    cTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCa         >  7:10453/1‑149 (MQ=255)
                                                                                                                                         ccGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAc    >  1:154250/1‑149 (MQ=255)
                                                                                                                                          cGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCAc    >  3:35336/1‑148 (MQ=255)
                                                                                                                                           gAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGa  <  4:27233/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
TCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGA  >  NC_000913/4529901‑4530188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.