Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,596,2230GC23.1% 50.6 / 14.7 26L61V (CTG→GTG) livHbranched‑chain amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (11/9);  new base C (3/3);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACTCCAGCCGTAGGCGCTGGCAATGACGATTGCGCCGACGAATCCCGCAGCTACCAGCAGCCAGCCGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAGGTACTGCCCAGCGTGACGCCGTTAAA  >  NC_000913/3596086‑3596367
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aaTCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACTCCAGCCGTAGGCGCTGGCAATGACGATTGCGCCGACGAATCCCGCAGCTACCAGCAGCCAGCCGGTATCAATGCCCATCATCATCAGCGCGGCgatga                                                                                                                                       >  6:98856/1‑149 (MQ=255)
             gagTTACGCACCGGGCGGTAAGCCACCCGTTCGATACTCCAGCCGTAGGCGCTGGCAATGACGATTGCGCCGACGAATCCCGCAGCTACCAGCAGCCAGCCGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATgaga                                                                                                                           <  5:98856/148‑1 (MQ=255)
                                       cagTTCGATACTCCAGCCGTAGGCGCTGGCAATGACGATTGCGCCGACGAATCCCGCAGCTACCAGCAGCCAGCCTGTATCAATGCCCATCATCATCCGCGCGGCGATGATCATAAATCAGACGTAGCt                                                                                                                    >  2:109458/3‑129 (MQ=255)
                                                     aGCCGTAGGCGCTGGCAATGACGATTGCGCCGACGAATCCCGCAGCTACCAGCAGCCAGCCGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTg                                                                                  <  2:28879/149‑1 (MQ=255)
                                                                                       gaATCCCGCAGCTACCAGCAGCCAGCCGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATgg                                                      <  6:4030/143‑1 (MQ=255)
                                                                                       gaATCCCGCAGCTACCAGCAGCCAGCCGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATgg                                                      >  5:4030/1‑143 (MQ=255)
                                                                                                 gCTACCAGCAGCCAGCCGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTAt                                      <  2:84358/149‑1 (MQ=255)
                                                                                                        gcagcCAGCCGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATc                                                                               >  6:85644/1‑101 (MQ=255)
                                                                                                        gcagcCAGCCGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGcgcg                               >  2:152768/1‑149 (MQ=255)
                                                                                                             cagccGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAgg                           >  1:82051/1‑148 (MQ=255)
                                                                                                             cagccGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAg                            <  2:74876/147‑1 (MQ=255)
                                                                                                             cagccGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAg                            >  1:74876/1‑147 (MQ=255)
                                                                                                              agccGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGaa                                                                                      >  2:28342/1‑88 (MQ=255)
                                                                                                              agccGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGaa                                                                                      <  1:28342/88‑1 (MQ=255)
                                                                                                                 cGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCata                                                                                                          >  3:126442/1‑65 (MQ=255)
                                                                                                                 cGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCata                                                                                                          <  4:126442/65‑1 (MQ=255)
                                                                                                                           atttatgatcatcgCCGCGCTGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAGGTACTGCCCAGCGTGa            >  8:99998/8‑149 (MQ=255)
                                                                                                                           atttatgatcatcgCCGCGCTGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAGGTACTGCCCAGCGTGa            <  7:99998/142‑1 (MQ=255)
                                                                                                                             ttatgatcatcgCCGCGCTGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGgtg                                                    <  8:32757/102‑1 (MQ=255)
                                                                                                                             ttatgatcatcgCCGCGCTGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGgtg                                                    >  7:32757/6‑107 (MQ=255)
                                                                                                                             ttatgatcatcgCCGCGCTGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGgtg                                                    <  6:55328/102‑1 (MQ=255)
                                                                                                                             ttatgatcatcgCCGCGCTGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGgtg                                                    >  5:55328/6‑107 (MQ=255)
                                                                                                                              catcatcatCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATg                                                       <  2:145203/103‑1 (MQ=255)
                                                                                                                              catcatcatCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATg                                                       >  1:145203/1‑103 (MQ=255)
                                                                                                                                  atcatcaGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAGGTACTGCCCAGCGTGACGCCGt      <  1:152768/148‑1 (MQ=255)
                                                                                                                                      tcaGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAGGTACTGCCCAGCGTGACGCCGTTaaa  >  3:889/1‑148 (MQ=255)
                                                                                                                                         |                                                                                                                                                
AATCAGGCGCTTAGAGTTACGCACCGGGCGGTAAGCCACCCGTTCGATACTCCAGCCGTAGGCGCTGGCAATGACGATTGCGCCGACGAATCCCGCAGCTACCAGCAGCCAGCCGGTATCAATGCCCATCATCATCAGCGCGGCGATGATCATAAATGAGACGTAGCTGCCAATCATATAAACCTCGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAGGTACTGCCCAGCGTGACGCCGTTAAA  >  NC_000913/3596086‑3596367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: