Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258674 258683 10–776 110 [0] [2] 111 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)97 (1.140) 69/228 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 111 total aligned reads displayed.

TGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTC  >  NC_000913/258541‑258827
                                                                                                                                               |                                                                                                                                               
gtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGt                                                                                                                                               <  5:180012‑M2/3‑1 (MQ=255)
   ttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaa                                                                                                                                           <  5:347543‑M2/7‑1 (MQ=255)
     aaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAg                                                                                                                                         <  5:12780‑M2/9‑1 (MQ=255)
      aaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggtcacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGa                                                                                                                                        >  6:333930‑M2/138‑147 (MQ=255)
      aaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGa                                                                                                                                        >  6:286354‑M2/138‑147 (MQ=255)
      aaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGa                                                                                                                                        >  8:169805‑M2/138‑147 (MQ=255)
      aaaatttgctaaattttgccaatttggtaaaacagttgaatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGa                                                                                                                                        <  2:66162‑M2/10‑1 (MQ=255)
       aaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGAt                                                                                                                                       >  5:267450‑M2/137‑147 (MQ=255)
        aatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagctatgacgttaccgagttgacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATaa                                                                                                                                     <  6:109587‑M2/13‑1 (MQ=255)
           tttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaattaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCg                                                                                                                                  <  7:393982‑M2/16‑1 (MQ=255)
                atattttgccaatttggtaaaacagttgcatcacaacacgagatagcaatgacgttaccgagaggacacccgatgaggagattgatcaaaaaatttaccgcactaggcccgtattttcgtcaaggttAGTGCAATGATAATCGATTCt                                                                                                                             >  3:392223‑M2/128‑148 (MQ=255)
                aaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcaccaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                                                             >  7:332624‑M2/128‑148 (MQ=255)
                   ttttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCtttt                                                                                                                          <  5:286354‑M2/24‑1 (MQ=255)
                   ttttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCtttt                                                                                                                          <  3:115000‑M2/24‑1 (MQ=255)
                        ccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                                                                <  8:23142‑M2/2‑1 (MQ=255)
                        ccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                                                                >  7:23142‑M2/120‑121 (MQ=255)
                            tttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                                                                 >  6:436806‑M2/116‑148 (MQ=255)
                            tttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                                                                 >  7:57116‑M2/116‑148 (MQ=255)
                                gtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                                                             >  1:427089‑M2/112‑148 (MQ=255)
                                   aaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaatagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                          <  8:409261‑M2/40‑1 (MQ=255)
                                   aaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                          <  4:272104‑M2/40‑1 (MQ=255)
                                   aaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                          <  2:445482‑M2/40‑1 (MQ=255)
                                    aacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                                                                         <  7:29932‑M2/41‑1 (MQ=255)
                                    aacagttgcatcacaacaggagatagcaatgacgttaccgagtggacaaccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                                                                         <  5:196000‑M2/41‑1 (MQ=255)
                                       aggtgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGc                                                                                                      >  3:164692‑M2/105‑148 (MQ=255)
                                          tgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttacagcagtaggcccgtatattcgtgaaggttAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                                                                    >  6:301630‑M2/102‑147 (MQ=255)
                                            catcacaacaggagatagcaatgacgttaccgagtggtcacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                                                                 <  5:333930‑M2/49‑1 (MQ=255)
                                            catcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                                                                 <  7:247313‑M2/49‑1 (MQ=255)
                                            catcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcacaaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                                                                 <  8:332624‑M2/49‑1 (MQ=255)
                                              tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgatggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACg                                                                                               >  6:322228‑M2/98‑148 (MQ=255)
                                              tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACg                                                                                               <  8:293802‑M2/51‑1 (MQ=255)
                                               cacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                                                              >  3:206962‑M2/97‑148 (MQ=255)
                                                acaaccggagattgctatgacgttaacgattggacacacgaagtgctgattgatcaaaaaattttccgctctaggcccgtttattcgtgaaggtaAGTGCAAAGATATTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                                                              <  1:328084‑M2/52‑1 (MQ=255)
                                                   acaggagatagcaatgacgttaccgagtggacacccgaagagcagtttgatcaaaaaatttaccgctctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaaa                                                                                          <  3:213519‑M2/56‑1 (MQ=255)
                                                   acaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACg                                                                                               >  7:8491‑M2/93‑143 (MQ=255)
                                                    caggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAc                                                                                         <  6:267450‑M2/57‑1 (MQ=255)
                                                     aggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                        <  2:427089‑M2/58‑1 (MQ=255)
                                                           tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttacctcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAcc                                                                                  >  3:185957‑M2/85‑148 (MQ=255)
                                                           tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTg                                                                                                           <  1:332503‑M2/39‑1 (MQ=255)
                                                           tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTg                                                                                                           >  2:332503‑M2/85‑123 (MQ=255)
                                                           tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCGcc                                                                                  >  5:388053‑M2/85‑148 (MQ=255)
                                                            agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                          <  1:204204‑M2/40‑1 (MQ=255)
                                                            agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                          >  2:204204‑M2/84‑123 (MQ=255)
                                                                 tgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                            >  2:95568‑M2/79‑148 (MQ=255)
                                                                    cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCg                                                                         >  1:270082‑M2/76‑148 (MQ=255)
                                                                            agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt                                                                 <  4:185957‑M2/81‑1 (MQ=255)
                                                                             agtggacacccgaagagcagattgatcaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt                                                                 <  6:433643‑M2/81‑1 (MQ=255)
                                                                                  cccccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCt                                                           >  1:39860‑M2/62‑148 (MQ=255)
                                                                                    cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGctga                                                         <  4:297354‑M2/89‑2 (MQ=255)
                                                                                      cgaagagcagattgattaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                       >  7:17100‑M2/58‑148 (MQ=255)
                                                                                       gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                       >  2:43852‑M2/57‑147 (MQ=255)
                                                                                        aagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtgga                                                     >  7:201069‑M2/56‑148 (MQ=255)
                                                                                           agcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                              >  1:50229‑M2/53‑88 (MQ=255)
                                                                                           agcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                              <  2:50229‑M2/36‑1 (MQ=255)
                                                                                            gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                    >  4:155581‑M2/52‑81 (MQ=255)
                                                                                            gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                    <  3:155581‑M2/30‑1 (MQ=255)
                                                                                              agattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc                                               <  1:95568‑M2/99‑1 (MQ=255)
                                                                                               gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGAGCAAAGATAATCGAGTCTTTTTCGATTGTCTGTATGTATGCTTCAAAGTGAAACCGGCACCGTAACTGCGTGAATTCTGTGGCTGGTGGTTGGAg                                                >  8:414737‑M2/49‑146 (MQ=255)
                                                                                                attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGAGTGAATTCTGGGGCTGGTGGATGGAGCTg                                             >  7:430332‑M2/48‑148 (MQ=255)
                                                                                                  tgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTgg                                            >  3:357404‑M2/46‑147 (MQ=255)
                                                                                                   gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGa                                                                               <  7:164109‑M2/67‑1 (MQ=255)
                                                                                                    atcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTGTTCGATTGTCTGGCTGTATGCGTCACCGTGCAACCGGCCCCGGaa                                                                              >  8:164109‑M2/44‑111 (MQ=255)
                                                                                                      caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGaa                                                                              <  2:428161‑M2/68‑1 (MQ=255)
                                                                                                        aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                     <  1:43852‑M2/109‑1 (MQ=255)
                                                                                                           aatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGa                                  <  5:436806‑M2/112‑1 (MQ=255)
                                                                                                                  cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                           <  1:93772‑M2/119‑1 (MQ=255)
                                                                                                                  cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                           <  2:270082‑M2/119‑1 (MQ=255)
                                                                                                                   gcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTGGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtt                          <  7:169805‑M2/120‑1 (MQ=255)
                                                                                                                      ctaggcccgtctctgcgtgaaggtcAGTGCAAAGAGAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAACCCGGCACCGGAAGTGCGGGAATTCTGGGGCGGGTGGATGGAGCTGGCAGCGCAGGACTCCCGTTTTa                       <  4:225993‑M2/123‑1 (MQ=255)
                                                                                                                       taggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                                                                 >  6:238149‑M2/25‑57 (MQ=255)
                                                                                                                       taggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                                                                 <  5:238149‑M2/33‑1 (MQ=255)
                                                                                                                       taggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtg                                                          <  1:147537‑M2/88‑1 (MQ=255)
                                                                                                                       taggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtg                                                          >  2:147537‑M2/25‑112 (MQ=255)
                                                                                                                         ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACTGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAcc                     >  4:368986‑M2/23‑147 (MQ=255)
                                                                                                                             cgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAg                >  6:275027‑M2/19‑148 (MQ=255)
                                                                                                                              gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGTGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGt               <  1:327803‑M2/131‑1 (MQ=255)
                                                                                                                              gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGTGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGt               >  2:327803‑M2/18‑148 (MQ=255)
                                                                                                                              gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGt               >  8:201606‑M2/18‑148 (MQ=255)
                                                                                                                              gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGt               >  3:271396‑M2/18‑148 (MQ=255)
                                                                                                                               tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGCCTGGTGGATGGAGCTGGAAGCGCAgg                                   <  4:219419‑M2/111‑1 (MQ=255)
                                                                                                                               tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGCCTGGTGGATGGAGCTGGAAGCGCAgg                                   >  3:219419‑M2/17‑127 (MQ=255)
                                                                                                                               tatattcgtgaaggtaAGTGAAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGtt              <  4:271396‑M2/132‑1 (MQ=255)
                                                                                                                                atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg                                                      <  2:137596‑M2/92‑1 (MQ=255)
                                                                                                                                atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg                                                      >  1:137596‑M2/16‑107 (MQ=255)
                                                                                                                                atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTg                                             >  1:147488‑M2/16‑116 (MQ=255)
                                                                                                                                atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTg                                             <  2:147488‑M2/101‑1 (MQ=255)
                                                                                                                                atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcgc                                      <  4:137465‑M2/108‑1 (MQ=255)
                                                                                                                                atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcgc                                      >  3:137465‑M2/16‑123 (MQ=255)
                                                                                                                                 tattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTa             <  7:201606‑M2/133‑1 (MQ=255)
                                                                                                                                   ttcgtgaaggtaAGTGCAAAGATACTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAcc           <  8:448558‑M2/135‑1 (MQ=255)
                                                                                                                                   ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                           >  7:423944‑M2/13‑131 (MQ=255)
                                                                                                                                   ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                           <  4:290367‑M2/119‑1 (MQ=255)
                                                                                                                                   ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                           >  3:290367‑M2/13‑131 (MQ=255)
                                                                                                                                   ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                           <  8:423944‑M2/119‑1 (MQ=255)
                                                                                                                                   ttcgggtaGGTAAGTGCAAAGATAATCGATTGTTTTTCGTTTGTCTGGATGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAATCGCAGGAACCCCGTTTTACCTACAGTTAcc           <  4:164692/140‑1 (MQ=255)
                                                                                                                                      gtgaaggtaAGTGCAAAGATAATCGATTCATTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGTCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGt        <  2:39860‑M2/138‑1 (MQ=255)
                                                                                                                                       tgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt      >  8:427865‑M2/9‑148 (MQ=255)
                                                                                                                                           ggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                                                                  >  4:234688‑M2/5‑84 (MQ=255)
                                                                                                                                           ggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                                                                  <  3:234688‑M2/80‑1 (MQ=255)
                                                                                                                                            gTAAGTGCAAAGATAATCCCTACTTTTTCGATTGTCAGGCTGTATGCGTCAACGTGATACCGGCTCCTGAAGTGCGTGAATTCTGGGGCTGGTGGACGGAGCTGGAAGCGCAGGAATCCCCTTTTACCTTCACTTACCAGTTTgggc  <  4:392223/147‑3 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTC  >  NC_000913/258541‑258827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: