Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 648,709 A→T 5.1% F288Y (TTC→TAC)  citF ← citrate lyase, citrate‑ACP transferase (alpha) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913648,7090AT 5.1% 261.2 / 3.5 79F288Y (TTC→TAC) citFcitrate lyase, citrate‑ACP transferase (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base A (36/39);  new base T (2/2);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

GCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTAGTGGTCATACGGGTCGCGC  >  NC_000913/648563‑648850
                                                                                                                                                  |                                                                                                                                             
gCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGa                                                                                                                                               >  5:362524/1‑147 (MQ=255)
   aTTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCACCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACg                                                                                                                                           <  2:335471/148‑1 (MQ=255)
        gcagcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTa                                                                                                                                      >  6:344164/1‑148 (MQ=255)
        gcagcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTa                                                                                                                                      >  7:173788/1‑148 (MQ=255)
                     cAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCCACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGAGgc                                                                                                                         >  2:321502/1‑148 (MQ=255)
                       acgCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATAGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATgcgc                                                                                                                       >  4:251028/3‑148 (MQ=255)
                       aaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTCTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATgcgc                                                                                                                       >  5:309904/1‑148 (MQ=255)
                               cacaTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCgccggtg                                                                                                               <  8:173788/148‑1 (MQ=255)
                               cacaTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCgccggtg                                                                                                               >  5:342314/1‑148 (MQ=255)
                               cacaTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGGCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCgccgg                                                                                                                 <  2:430223/146‑1 (MQ=255)
                                  aTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGTAACGGGTTACCGCCAGCGATGCGCCgccggtgccg                                                                                                            >  6:101765/1‑148 (MQ=255)
                                  aTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGTAACGGGTTACCGCCAGCGATGCGCCgccggtgccg                                                                                                            >  6:107816/1‑148 (MQ=255)
                                     cagcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGtt                                                                                                         <  4:70334/148‑1 (MQ=255)
                                     cagcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGtt                                                                                                         >  8:428713/1‑148 (MQ=255)
                                     cagcagTTTGCGGATCAGACCTCTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGtt                                                                                                         <  6:309904/148‑1 (MQ=255)
                                            ttGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGa                                                                                                                            <  6:98306/122‑1 (MQ=255)
                                            ttGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGa                                                                                                                            >  5:98306/1‑122 (MQ=255)
                                             tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATgg                                                                                                  <  6:342314/147‑1 (MQ=255)
                                             tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATgg                                                                                                  <  1:213087/147‑1 (MQ=255)
                                                   tCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGaa                                                                                                                                              <  7:152912/97‑1 (MQ=255)
                                                   tCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGaa                                                                                                                                              >  8:152912/1‑97 (MQ=255)
                                                      gACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACcttc                                                                                        >  4:345247/1‑148 (MQ=255)
                                                       aCCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACcttct                                                                                       >  6:345951/1‑148 (MQ=255)
                                                       aCCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACcttct                                                                                       >  4:224891/1‑148 (MQ=255)
                                                        ccTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACcttctt                                                                                      >  7:100599/1‑148 (MQ=255)
                                                         cTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCttt                                                                                     <  3:224891/148‑1 (MQ=255)
                                                          tttttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCttt                                                                                     >  7:51865/1‑147 (MQ=255)
                                                            tttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGa                                                                                   <  5:345951/147‑1 (MQ=255)
                                                            tttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGGCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGa                                                                                   <  5:344164/147‑1 (MQ=255)
                                                             ttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGaa                                                                                  <  3:345247/147‑1 (MQ=255)
                                                               cGTTCAGGTCAACCATCGTCGAGGTAATACCGCCAAGGTCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAAGGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGTATGGAGAAACCTTCTTTGAAGt                                                                                <  8:99806/147‑1 (MQ=255)
                                                               cGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGt                                                                                <  5:424739/147‑1 (MQ=255)
                                                                gggCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAg                                                                              <  5:254607/146‑1 (MQ=255)
                                                                  gCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGCAACCTTCTTTGAAGTAGc                                                                             >  1:335970/1‑147 (MQ=255)
                                                                   cAGGTCAACCATCGTCGCGGTAATTCCGCCAAGTGCGAAGTCGGCGCGAATATCTCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCa                                                                           <  7:57265/148‑1 (MQ=255)
                                                                   cAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGTAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCa                                                                           <  5:101765/148‑1 (MQ=255)
                                                                   cAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGTAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCa                                                                           <  5:107816/148‑1 (MQ=255)
                                                                      gTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCagag                                                                        <  6:362524/148‑1 (MQ=255)
                                                                      gTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCagag                                                                        >  3:337608/1‑148 (MQ=255)
                                                                       tCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGt                                                                       >  2:235295/1‑148 (MQ=255)
                                                                            cATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGAc                                                                   >  2:427703/1‑147 (MQ=255)
                                                                              tcgtcgCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAAt                                                                <  7:428713/148‑1 (MQ=255)
                                                                                  cgcgGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCttt                                                                                     <  8:335402/123‑1 (MQ=255)
                                                                                  cgcgGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCttt                                                                                     >  7:335402/1‑123 (MQ=255)
                                                                                   gcgGTAATACCGCCAAGGGCGAAGTCGGAGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATcaca                                                            <  4:337608/147‑1 (MQ=255)
                                                                                       tAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACctt                                                                                         >  4:52904/1‑114 (MQ=255)
                                                                                       tAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACctt                                                                                         <  3:52904/114‑1 (MQ=255)
                                                                                           aCCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGaa                                                                                                                                              >  1:314558/1‑57 (MQ=255)
                                                                                           aCCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGaa                                                                                                                                              <  2:314558/57‑1 (MQ=255)
                                                                                           aCCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACcttct                                                                                       >  1:405348/1‑112 (MQ=255)
                                                                                           aCCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACcttct                                                                                       <  2:405348/112‑1 (MQ=255)
                                                                                           aCCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACAGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATTGAGAAACCTTCTTTGa                                                                                   >  4:299047/1‑116 (MQ=255)
                                                                                           aCCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACAGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGa                                                                                   <  3:299047/116‑1 (MQ=255)
                                                                                            ccGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGc                                                                                                      <  7:322495/96‑1 (MQ=255)
                                                                                               ccAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTAc                                               <  8:51865/148‑1 (MQ=255)
                                                                                                       gAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAg                                                                              <  8:327071/109‑1 (MQ=255)
                                                                                                       gAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAg                                                                              >  7:327071/1‑109 (MQ=255)
                                                                                                         aGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGc                                                                                                      >  3:374200/1‑83 (MQ=255)
                                                                                                         aGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGc                                                                                                      <  4:374200/83‑1 (MQ=255)
                                                                                                         aGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAg                                     <  8:100599/148‑1 (MQ=255)
                                                                                                          gTCGGCGCGAATATCGCGGCTACTCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCACCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACTGGCAATAAGc                                    <  8:89351/148‑1 (MQ=255)
                                                                                                             ggCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGt                                 <  8:318660/148‑1 (MQ=255)
                                                                                                                 cgAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCATCGCTACGGGCAATAAGCAGTTc                               >  7:339737/1‑146 (MQ=255)
                                                                                                                        cgcgGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGc                                                                                                      <  5:408431/68‑1 (MQ=255)
                                                                                                                        cgcgGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGc                                                                                                      >  6:408431/1‑68 (MQ=255)
                                                                                                                         gcgGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTa                     <  8:339737/148‑1 (MQ=255)
                                                                                                                               aCGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTAGTGGt                >  2:129617/1‑147 (MQ=255)
                                                                                                                                cGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATCAGCAGTTCGCGCGGGTTAGTGGTCa              >  4:198161/1‑148 (MQ=255)
                                                                                                                                cGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTAGTGGTc               <  1:427703/147‑1 (MQ=255)
                                                                                                                                 gCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTAGTGGTCAt             <  1:321502/148‑1 (MQ=255)
                                                                                                                                     tttGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCgccggtgccggt                                                                                                          >  1:99353/1‑51 (MQ=255)
                                                                                                                                     tttGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCgccggtgccggt                                                                                                          <  2:99353/51‑1 (MQ=255)
                                                                                                                                     tttGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGa                                                                                   >  4:148823/1‑74 (MQ=255)
                                                                                                                                     tttGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGa                                                                                   <  3:148823/74‑1 (MQ=255)
                                                                                                                                     tttGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTa                     >  4:328494/1‑136 (MQ=255)
                                                                                                                                     tttGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTa                     <  3:328494/136‑1 (MQ=255)
                                                                                                                                     tttGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTAGTGGTCAt             <  5:111979/144‑1 (MQ=255)
                                                                                                                                     tttGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTAGTGGTCAt             >  6:111979/1‑144 (MQ=255)
                                                                                                                                          cTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACg                                              >  1:53186/1‑106 (MQ=255)
                                                                                                                                          cTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACg                                              <  2:53186/106‑1 (MQ=255)
                                                                                                                                            tCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAGTCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTAGTGGTCATACGGGTCgcgc  >  5:236722/1‑148 (MQ=255)
                                                                                                                                            tCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTAGTGGTCATACGGGTCgcgc  >  1:419448/1‑148 (MQ=255)
                                                                                                                                            tCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTAGTGGTCATACGGGTCgcgc  >  8:405089/1‑148 (MQ=255)
                                                                                                                                            tCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTAGTGGTCATACGGGTCgcgc  >  5:7249/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
GCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGAAACGGGTTACCGCCAGCGATGCGCCGCCGGTGCCGGTTTGCATGGAGAAACCTTCTTTGAAGTAGCCAGAGTTGACAATCACATCCGCAGCGCTACGGGCAATAAGCAGTTCGCGCGGGTTAGTGGTCATACGGGTCGCGC  >  NC_000913/648563‑648850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: