Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,212,074 T→C 5.5% intergenic (‑71/+629) iraM ← / ← ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,212,0740TC 5.5% 226.5 / 2.2 73intergenic (‑71/+629)iraM/ycgXRpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
Reads supporting (aligned to +/- strand):  ref base T (42/27);  new base C (2/2);  total (44/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTA  >  NC_000913/1211932‑1212219
                                                                                                                                              |                                                                                                                                                  
atTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTatat                                                                                                                                                >  8:300/1‑147 (MQ=255)
          atGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCt                                                                                                                                     >  8:442448/1‑148 (MQ=255)
          atGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCt                                                                                                                                     >  7:186245/1‑148 (MQ=255)
                  aaaaGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCaaaaaaa                                                                                                                            <  2:261077/148‑1 (MQ=255)
                    aaGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAaaat                                                                                                                           >  6:416217/1‑147 (MQ=255)
                        tataCCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGtat                                                                                                                                                  <  5:388654/121‑1 (MQ=255)
                        tataCCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGtat                                                                                                                                                  >  6:388654/1‑121 (MQ=255)
                           aCCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAaattaat                                                                                                                   >  6:118741/1‑148 (MQ=255)
                           aCCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAaattaa                                                                                                                    <  1:387834/147‑1 (MQ=255)
                           aCCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAaattaa                                                                                                                    >  2:387834/1‑147 (MQ=255)
                            ccACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAaattaatt                                                                                                                  <  8:426096/148‑1 (MQ=255)
                             cacaTGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAaattaatt                                                                                                                  >  8:141094/1‑147 (MQ=255)
                               caTGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCGCCGCAAAAAAATAAAttaattaa                                                                                                                >  2:370382/1‑147 (MQ=255)
                                   tAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAatga                                                                                                           >  1:353187/1‑148 (MQ=255)
                                     ggTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATATAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAatgat                                                                                                          <  7:236757/147‑1 (MQ=255)
                                       ttGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGc                                                                                                       >  7:225380/1‑148 (MQ=255)
                                       ttGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGc                                                                                                       >  3:3381/1‑148 (MQ=255)
                                          aaTTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTta                                                                                                    >  7:367795/1‑148 (MQ=255)
                                           aTTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTAATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCATCAAAAAAAAAAATAAATAAAAATCATggggta                                                                                                    >  6:13193/1‑143 (MQ=255)
                                               ccGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTtatata                                                                                                >  7:265674/1‑147 (MQ=255)
                                                cGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATataaa                                                                                              >  1:31505/1‑148 (MQ=255)
                                                  tgtCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGATTATATTAGACCCCCATAAAAAAAATTATTTAATTTATCTGATGGCTTATataaaa                                                                                             >  5:239544/1‑147 (MQ=255)
                                                  tgtCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGGTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATataaaa                                                                                             >  4:135960/1‑147 (MQ=255)
                                                   gtCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGtat                                                                                                                                                  >  3:309347/1‑94 (MQ=255)
                                                   gtCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGtat                                                                                                                                                  <  4:309347/94‑1 (MQ=255)
                                                        ttACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAAtt                                                                                      >  2:338247/1‑148 (MQ=255)
                                                           cTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTa                                                                                    <  7:442448/147‑1 (MQ=255)
                                                             aTCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAg                                                                                 >  3:32634/1‑148 (MQ=255)
                                                               ccACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAAATAAAATGATGGCTTAATTAATAAAAAATTTAAAGc                                                                                >  4:344147/1‑147 (MQ=255)
                                                                caaatCATTTGGTTAGTCGTTTCCCACGGTATTTAAATTTGTTCATTACTATTTTTAATTCTTGATTTTTCATATGATTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCaa                                                                              <  5:13193/144‑1 (MQ=255)
                                                                cACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCa                                                                               <  4:3381/147‑1 (MQ=255)
                                                                cACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCa                                                                               <  8:265674/147‑1 (MQ=255)
                                                                  cTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAgg                                                                            <  5:416217/148‑1 (MQ=255)
                                                                  cTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAAGTAAAATGATGGCTGTTATAAAATAAAATTTAAAGCAAg                                                                             >  4:277316/1‑147 (MQ=255)
                                                                   ttCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAATTTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAgg                                                                            >  4:236017/1‑147 (MQ=255)
                                                                   ttCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGGTGGCTTATATAAAATAAAATTTaaa                                                                                  >  6:405567/1‑141 (MQ=255)
                                                                   ttCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGGTGGCTTATATAAAATAAAATTTaaa                                                                                  <  5:405567/141‑1 (MQ=255)
                                                                    tCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaa                                                                                   >  2:280180/1‑139 (MQ=255)
                                                                    tCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaa                                                                                   <  1:280180/139‑1 (MQ=255)
                                                                          gTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCa                                                                     <  5:118741/147‑1 (MQ=255)
                                                                          gTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCa                                                                     <  1:338247/147‑1 (MQ=255)
                                                                              tGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGa                                                                <  8:186245/148‑1 (MQ=255)
                                                                              tGTCTTATCCAACGGTATTTAATATGTGTCATTATGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGa                                                                <  6:239544/148‑1 (MQ=255)
                                                                                tCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGCCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATg                                                              >  8:260378/1‑148 (MQ=255)
                                                                                        cACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACa                                                       >  1:386752/1‑147 (MQ=255)
                                                                                           ggTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAAt                                                   >  3:345586/1‑148 (MQ=255)
                                                                                                   atatGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                            <  8:225380/147‑1 (MQ=255)
                                                                                                     atGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTTCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAATATTTAAAGCAAGGAATCTCAATGTATGTTAAACAAAATGATATTTtgt                                         >  1:391162/1‑148 (MQ=255)
                                                                                                       ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGATTATATAAAATAAAATTTAAAGAAAGGAATATAAATGGATGTTAAACAAAATTTGATTTtgtg                                        >  2:394468/1‑147 (MQ=255)
                                                                                                        gTTCATTAGTATGTTTATTTCTTGATTTTGCATATGAGTATATTACACCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGaa                                      <  5:340714/148‑1 (MQ=255)
                                                                                                         ttCATTAGGATTTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCACCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTtg                                          <  4:43537/143‑1 (MQ=255)
                                                                                                          tCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTTCCCCCCCCTCAAAAAATTAATTTAATTTAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGATTGTCTATGGATGTTAATCTATATGAGATTTTGTGagat                                    >  3:43537/1‑145 (MQ=255)
                                                                                                             ttAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCaa                                 >  8:356830/1‑148 (MQ=255)
                                                                                                             ttAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCaa                                 >  2:359381/1‑148 (MQ=255)
                                                                                                               aGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAAt                                >  7:379721/1‑147 (MQ=255)
                                                                                                                ggATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATaa                              <  4:345586/148‑1 (MQ=255)
                                                                                                                    gTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAatta                          >  7:188808/1‑148 (MQ=255)
                                                                                                                     tttATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAattat                         <  2:31505/148‑1 (MQ=255)
                                                                                                                       tATTTCTTGATTTTGCATATGAGCATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCaa                                                                    <  7:291090/103‑1 (MQ=255)
                                                                                                                       tATTTCTTGATTTTGCATATGAGCATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCaa                                                                    <  7:298212/103‑1 (MQ=255)
                                                                                                                       tATTTCTTGATTTTGCATATGAGCATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCaa                                                                    >  8:291090/1‑103 (MQ=255)
                                                                                                                       tATTTCTTGATTTTGCATATGAGCATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATATCaa                                                                    >  8:298212/1‑103 (MQ=255)
                                                                                                                        aTTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAattatt                        >  4:411286/1‑146 (MQ=255)
                                                                                                                        aTTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTg                       <  3:236017/147‑1 (MQ=255)
                                                                                                                              tGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCaa                                                                              <  6:103595/86‑1 (MQ=255)
                                                                                                                              tGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCaa                                                                              >  5:103595/1‑86 (MQ=255)
                                                                                                                              tGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATATCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTgcgttggt                >  4:39213/1‑141 (MQ=255)
                                                                                                                                aTTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACaa                                                      >  3:133948/1‑108 (MQ=255)
                                                                                                                                aTTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACaa                                                      <  4:133948/108‑1 (MQ=255)
                                                                                                                                  tttGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGttt              >  7:212354/1‑146 (MQ=255)
                                                                                                                                    tGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAg           <  7:141094/147‑1 (MQ=255)
                                                                                                                                     gCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAATTTATTGACTTCGTTTTAGa          <  1:359381/147‑1 (MQ=255)
                                                                                                                                         atGAGTATATTACCCCCACCTCAAAAAAATAAATTAATTAAAATGATCGCTTATATAAAATAAATTTTAAAGAAAGGAATATCAATGGATGTTAAACAAAAATAGATTTTCTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGt     >  4:185358/1‑148 (MQ=255)
                                                                                                                                          tGAGTATATTACCCCCCCCTCAAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGt     >  7:287844/1‑148 (MQ=255)
                                                                                                                                          tGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGtt    <  4:32634/148‑1 (MQ=255)
                                                                                                                                            aGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTTTGAAAGCAATAAATTATTGACTTCGTTTTTGATTTGtt    >  5:232561/1‑146 (MQ=255)
                                                                                                                                            aGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTa  <  3:277316/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
ATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTA  >  NC_000913/1211932‑1212219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: