Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,381,915 G→T 5.4% intergenic (+13/‑32) ycjV → / → ompG pseudogene; putative ATP‑binding component of a transport system/outer membrane porin G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,381,9150GT 5.4% 321.6 / 5.5 93intergenic (+13/‑32)ycjV/ompGpseudogene; putative ATP‑binding component of a transport system/outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base G (49/39);  new base T (3/2);  total (52/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

ACGGTTGGGGGCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGA  >  NC_000913/1381769‑1382059
                                                                                                                                                  |                                                                                                                                                
acGGTTGGGGGCCCGAGTTAGTGGTCCGTGCGGGTGCGTTAAATGATTATCATGCAGGAGACAATATCACTATTCATTTTGATATGACGAAAAGGCATTTCGGTGCTGCAGACACGGAAATAGCAATGCGCTACATACAGGGGGACgg                                                                                                                                                 >  8:200966/1‑148 (MQ=255)
acGGTTGGGGGCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATAACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAAAGGAAATAGCAATTAGCTAAATACAGGGGGAAgg                                                                                                                                                 >  7:421299/1‑148 (MQ=255)
         ggCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTcccc                                                                                                                                         >  2:46979/1‑147 (MQ=255)
          gCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCa                                                                                                                                       <  1:46979/148‑1 (MQ=255)
             cGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGa                                                                                                                                    >  2:404286/1‑148 (MQ=255)
               aGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGata                                                                                                                                  <  7:45787/148‑1 (MQ=255)
                  tAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGataata                                                                                                                               >  8:408387/1‑148 (MQ=255)
                    gTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATAc                                                                                                                              >  4:38429/1‑147 (MQ=255)
                    gTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACa                                                                                                                             <  6:65164/148‑1 (MQ=255)
                     tGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTccc                                                                                                                                          <  4:228739/134‑1 (MQ=255)
                     tGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTccc                                                                                                                                          >  3:228739/1‑134 (MQ=255)
                        tCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAgg                                                                                                                          >  8:432803/1‑147 (MQ=255)
                           gTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCAGTATTCATTTTGATATGACGAAATGTCATTTCTTTGCTGCAGAAACGGAAATAGCAAGTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAAc                                                                                                                       >  2:92501/1‑147 (MQ=255)
                            tGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACaa                                                                                                                     >  2:216509/1‑148 (MQ=255)
                                       tAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAg                                                                                                          >  5:175650/1‑148 (MQ=255)
                                        aaaTGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGt                                                                                                         >  8:244203/1‑148 (MQ=255)
                                         aaTGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGtt                                                                                                        <  6:175650/148‑1 (MQ=255)
                                          aTGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGtta                                                                                                       >  1:436288/1‑148 (MQ=255)
                                           tGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGttat                                                                                                      >  2:314192/1‑148 (MQ=255)
                                               tATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTAcc                                                                                                  >  6:39146/1‑148 (MQ=255)
                                                  cATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGt                                                                                                         <  6:52556/138‑1 (MQ=255)
                                                  cATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGt                                                                                                         >  5:52556/1‑138 (MQ=255)
                                                    tGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGTGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTa                                                                                             <  2:216388/148‑1 (MQ=255)
                                                     gCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGc                                                                                                                                                <  4:396383/96‑1 (MQ=255)
                                                     gCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGc                                                                                                                                                >  3:396383/1‑96 (MQ=255)
                                                     gCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGataat                                                                                                                                >  7:117466/1‑112 (MQ=255)
                                                     gCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGataat                                                                                                                                <  8:117466/112‑1 (MQ=255)
                                                          agaAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGaaaa                                                                                                            >  3:297962/1‑127 (MQ=255)
                                                          agaAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGaaaa                                                                                                            <  4:297962/127‑1 (MQ=255)
                                                          agaAAATATCACTATTCAGTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCAc                                                                                       >  6:150107/1‑148 (MQ=255)
                                                            aaaaTATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACTGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTAccc                                                                                                 <  1:176269/136‑1 (MQ=255)
                                                            aaaaTATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACTGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTAccc                                                                                                 >  2:176269/1‑136 (MQ=255)
                                                             aaaTATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTg                                                                                     <  2:436288/147‑1 (MQ=255)
                                                             aaaTATCAATATTCATTTTGATATGACGATATGTAATTTCTTTGATGCAGAAACTGAAATAGCAATTAGCTAATTAGATGGGGAAGTCTTTCCCCGAGGATAATACATGGAAGAATAATGAAAAAGTTTTTACCCTGTAGCGCACTg                                                                                     <  8:421299/147‑1 (MQ=255)
                                                              aaTATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGt                                                                                   >  8:318925/1‑148 (MQ=255)
                                                                tatCCCTATTCATTTTGATATGCCGAAATGTCATTTCTTTGATGCAGAAACGGCAATAGCTATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATTATACAAGGCACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGGGa                                                                                 <  7:200966/148‑1 (MQ=255)
                                                                  tCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTg                                                                                     <  3:399630/142‑1 (MQ=255)
                                                                  tCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTg                                                                                     >  4:399630/1‑142 (MQ=255)
                                                                      tATTCGTTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAtgtgtg                                                                           >  4:139941/1‑148 (MQ=255)
                                                                       aTTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGc                                                                          >  5:76116/1‑148 (MQ=255)
                                                                       aTTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGc                                                                          >  8:366288/1‑148 (MQ=255)
                                                                        ttCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGc                                                                          <  7:318925/147‑1 (MQ=255)
                                                                         tCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAGGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTg                                                                                  >  8:440115/1‑138 (MQ=255)
                                                                         tCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAGGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTg                                                                                  <  7:440115/138‑1 (MQ=255)
                                                                            ttttGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTgg                                                                                    >  1:24473/1‑133 (MQ=255)
                                                                            ttttGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTgg                                                                                    <  2:24473/133‑1 (MQ=255)
                                                                            ttttGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAtg                                                                               <  6:11808/138‑1 (MQ=255)
                                                                            ttttGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAtg                                                                               >  5:11808/1‑138 (MQ=255)
                                                                                   atGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTg                                                              >  2:32250/1‑148 (MQ=255)
                                                                                    tGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTgc                                                             <  1:216509/148‑1 (MQ=255)
                                                                                     gACGAAATTTCATTTCTTTGATGCAGAAACGGAAATAGCAATTAGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCATGTACCTCACTGGTGATGTTGGCGGCAATCTCCTgcg                                                            >  8:284497/1‑148 (MQ=255)
                                                                                     gACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGc                                                                                         <  8:74393/119‑1 (MQ=255)
                                                                                     gACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGc                                                                                         >  7:74393/1‑119 (MQ=255)
                                                                                          aaTGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGc                                                                                         >  2:433087/1‑114 (MQ=255)
                                                                                          aaTGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGc                                                                                         <  1:433087/114‑1 (MQ=255)
                                                                                            tGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGc                                                     >  6:304972/1‑148 (MQ=255)
                                                                                             gTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGcc                                                    >  4:66298/1‑148 (MQ=255)
                                                                                                 tttCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGag                                                 >  4:123565/1‑147 (MQ=255)
                                                                                                 tttCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGag                                                 >  2:139581/1‑147 (MQ=255)
                                                                                                  ttCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTcccc                                                                                                                                         <  5:9759/58‑1 (MQ=255)
                                                                                                  ttCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTcccc                                                                                                                                         >  6:9759/1‑58 (MQ=255)
                                                                                                  ttCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCa                                                                                        <  4:24950/107‑1 (MQ=255)
                                                                                                  ttCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCa                                                                                        >  3:24950/1‑107 (MQ=255)
                                                                                                  ttCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGagg                                                >  1:215353/1‑147 (MQ=255)
                                                                                                  ttCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGagg                                                >  4:342184/1‑147 (MQ=255)
                                                                                                   tCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCTGGAATGGCCTGCGCACAGGCCGagga                                               <  3:139941/147‑1 (MQ=255)
                                                                                                    cTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGaggaa                                              <  3:66298/147‑1 (MQ=255)
                                                                                                           gCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACg                                       >  2:169108/1‑147 (MQ=255)
                                                                                                           gCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGa                                      >  7:111685/1‑148 (MQ=255)
                                                                                                               aaaCGGAAATAGCAATTCGCTAAATACAGGGGGTAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTgg                                  <  6:154751/148‑1 (MQ=255)
                                                                                                               aaaCGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTgg                                  >  3:359188/1‑148 (MQ=255)
                                                                                                                aaCGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTgg                                  >  6:147673/1‑147 (MQ=255)
                                                                                                                 aCGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCa                                <  7:408387/148‑1 (MQ=255)
                                                                                                                    gAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACtt                             <  1:169108/148‑1 (MQ=255)
                                                                                                                     aaaTAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACttt                            <  5:150107/148‑1 (MQ=255)
                                                                                                                        tAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAat                         <  3:267117/148‑1 (MQ=255)
                                                                                                                             aTTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCgg                    <  3:342184/148‑1 (MQ=255)
                                                                                                                             aTTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCgg                    <  1:314192/148‑1 (MQ=255)
                                                                                                                               tCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAg                                                                                                          <  5:318298/60‑1 (MQ=255)
                                                                                                                               tCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAg                                                                                                          >  6:318298/1‑60 (MQ=255)
                                                                                                                               tCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGa                                                                      >  2:234546/1‑96 (MQ=255)
                                                                                                                               tCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGgcg                  >  6:120790/1‑148 (MQ=255)
                                                                                                                               tCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAGGTGTGCGGGa                                                                      <  1:234546/96‑1 (MQ=255)
                                                                                                                                 gCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGcgcg                <  8:111685/148‑1 (MQ=255)
                                                                                                                                    aaaTACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACg                                       <  4:373376/122‑1 (MQ=255)
                                                                                                                                    aaaTACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACg                                       >  3:373376/1‑122 (MQ=255)
                                                                                                                                          tgggggAATGCCTTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGa        >  7:80667/2‑147 (MQ=255)
                                                                                                                                          tgggggAATGCCTTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGa        >  3:200245/2‑147 (MQ=255)
                                                                                                                                          aGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaa       <  1:404286/148‑1 (MQ=255)
                                                                                                                                           gggggAATGCCTTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaaa      >  6:333496/1‑148 (MQ=255)
                                                                                                                                           gggggAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCCCTTTAATATCGGCGCGATGTACGaaa      <  1:92501/148‑1 (MQ=255)
                                                                                                                                            ggggAATGCCTTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCTCTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaa       <  8:80667/146‑1 (MQ=255)
                                                                                                                                            ggggAATGCCTTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaa       <  4:200245/146‑1 (MQ=255)
                                                                                                                                             gggAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCTCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGTAAGGAACGACTGGCACTTTAATATCGGCGCGTTGTAGGaaa      >  5:350103/1‑146 (MQ=255)
                                                                                                                                                aaGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGa  >  1:201451/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACGGTTGGGGGCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGA  >  NC_000913/1381769‑1382059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: