Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,744,445 C→T 5.9% A330V (GCC→GTC)  mdtK → multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,744,4450CT 5.9% 217.4 / 2.7 68A330V (GCC→GTC) mdtKmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base C (31/33);  new base T (2/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

TCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGC  >  NC_000913/1744300‑1744591
                                                                                                                                                 |                                                                                                                                                  
tCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCa                                                                                                                                                  >  1:396618/1‑148 (MQ=255)
     aaTGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCAtttt                                                                                                                                              >  1:60988/1‑147 (MQ=255)
     aaTGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCAtttt                                                                                                                                              >  4:378564/1‑147 (MQ=255)
         ttCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACgg                                                                                                                                         <  1:197105/148‑1 (MQ=255)
         ttCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACg                                                                                                                                          <  7:54268/147‑1 (MQ=255)
             tGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTc                                                                                                                                     <  6:95857/148‑1 (MQ=255)
                  ccAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTg                                                                                                                                 <  8:432113/147‑1 (MQ=255)
                   cAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCg                                                                                                                               >  5:274919/1‑148 (MQ=255)
                    aaTGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTc                                                                                                                                     <  2:84832/141‑1 (MQ=255)
                    aaTGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGAGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTc                                                                                                                                     >  1:84832/1‑141 (MQ=255)
                       gTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAg                                                                                                                           >  4:244260/1‑148 (MQ=255)
                        tCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAg                                                                                                                           <  3:142098/147‑1 (MQ=255)
                           cTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCaaa                                                                                                                       >  8:261473/1‑148 (MQ=255)
                                    gCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCtgt                                                                                                              <  3:244260/148‑1 (MQ=255)
                                       gTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCtgttgt                                                                                                           <  2:60988/148‑1 (MQ=255)
                                        tAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTc                                                                                                                                     >  4:309657/1‑121 (MQ=255)
                                        tAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTc                                                                                                                                     <  3:309657/121‑1 (MQ=255)
                                              tCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCgg                                                                                                                              <  7:100953/122‑1 (MQ=255)
                                              tCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCgg                                                                                                                              >  8:100953/1‑122 (MQ=255)
                                              tCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACg                                                                                                     <  7:261473/147‑1 (MQ=255)
                                               ccGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGTCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGa                                                                                                    >  7:118083/1‑147 (MQ=255)
                                                    tAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATcc                                                                                              >  1:170869/1‑148 (MQ=255)
                                                      gggtATCGTCTGGGTCAGGGCGCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCg                                                                                            <  5:11915/145‑1 (MQ=255)
                                                      ggTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCTCTGGGCGGTCCGGGCTTATTGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCTCTGTGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCg                                                                                            >  1:393653/1‑148 (MQ=255)
                                                       gTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATccc                                                                                             >  4:50255/1‑146 (MQ=255)
                                                        ttATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAGCCCTGACGGCCATTTTGACGGTTTCACTGCGGGAGCAACTCGCCCTGTTGTACCACGACAATCCCGAg                                                                                          >  6:59980/1‑148 (MQ=255)
                                                              tCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTa                                                                                    <  2:396618/148‑1 (MQ=255)
                                                               cTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTaa                                                                                   >  7:15356/1‑148 (MQ=255)
                                                                         gCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACgctggc                                                                            <  6:425099/145‑1 (MQ=255)
                                                                         gCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACgctggc                                                                            >  5:425099/1‑145 (MQ=255)
                                                                         gCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTgc                                                                         >  3:377296/1‑148 (MQ=255)
                                                                           tCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTgcg                                                                        >  5:218809/1‑147 (MQ=255)
                                                                              aCGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAAAGACAATc                                                                                               >  6:25070/1‑121 (MQ=255)
                                                                              aCGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAAAGACAATc                                                                                               <  5:25070/121‑1 (MQ=255)
                                                                                     aTGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCg                                                                                                                               <  3:320919/82‑1 (MQ=255)
                                                                                     aTGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCg                                                                                                                               >  4:320919/1‑82 (MQ=255)
                                                                                        cgcATACCGCTGCGCGGACCGGGCTTATGGTGTGTGTCTGTATGGCATCCCTGACGGCCATTTTCACGGTTTCACTGCGGGTGCAAATCGCCCTGTTGTACTACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGc                                                           <  7:175180/147‑1 (MQ=255)
                                                                                        cgcAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGc                                                           <  5:355733/147‑1 (MQ=255)
                                                                                          cAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTgg                                                        >  4:180494/1‑148 (MQ=255)
                                                                                            aaCCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTggcg                                                      <  2:170869/148‑1 (MQ=255)
                                                                                             aCCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGTCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTggcgg                                                     <  8:118083/148‑1 (MQ=255)
                                                                                             aCCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTggcgg                                                     >  6:31652/1‑148 (MQ=255)
                                                                                                gCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGGACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGcggcgg                                                  <  6:274919/148‑1 (MQ=255)
                                                                                                        gACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGGTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGGTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAg                                          <  5:59980/148‑1 (MQ=255)
                                                                                                            gggCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACACCGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGAttt                                      >  3:159914/1‑148 (MQ=255)
                                                                                                            gggCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGt                                                 <  4:411270/137‑1 (MQ=255)
                                                                                                            gggCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGt                                                 >  3:411270/1‑137 (MQ=255)
                                                                                                             ggCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTc                                     >  2:376598/1‑148 (MQ=255)
                                                                                                                  aTGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGa                                  <  3:378564/146‑1 (MQ=255)
                                                                                                                    ggtgggtgTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTc                               >  6:271989/1‑147 (MQ=255)
                                                                                                                        ggtgTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATcc                          <  5:271989/148‑1 (MQ=255)
                                                                                                                           gtctgtctGGCAACCCTGACTGCCATTTTCACGTTTTCACTGCGGGAGCAAATCGCCCTGGTGTACAACGACAAACCCGAGGTTGTAACGCTGGCGGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAgg                       <  2:257887/148‑1 (MQ=255)
                                                                                                                           gtCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAg                        >  8:258109/1‑147 (MQ=255)
                                                                                                                           gtCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAg                        <  7:258109/147‑1 (MQ=255)
                                                                                                                            tCTGTATGGCAACCCTGACGGTCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAAt                                                                                                >  1:306013/1‑74 (MQ=255)
                                                                                                                            tCTGTATGGCAACCCTGACGGTCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAAt                                                                                                <  2:306013/74‑1 (MQ=255)
                                                                                                                               gTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTggcggc                                                    <  1:301407/115‑1 (MQ=255)
                                                                                                                               gTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTggcggc                                                    >  2:301407/1‑115 (MQ=255)
                                                                                                                               gTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGgcggcg                                                   >  6:295585/1‑116 (MQ=255)
                                                                                                                               gTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGgcggcg                                                   <  5:295585/116‑1 (MQ=255)
                                                                                                                                tATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTg                     <  1:376598/145‑1 (MQ=255)
                                                                                                                                tATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGAtt                  <  6:218809/148‑1 (MQ=255)
                                                                                                                                 aTGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGGAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTATCGCTGGCTGCGCATTTGATg                                                               <  7:218995/102‑1 (MQ=255)
                                                                                                                                 aTGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATg                                                               >  8:218995/1‑102 (MQ=255)
                                                                                                                                    gCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGc               <  4:377296/147‑1 (MQ=255)
                                                                                                                                    gCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCa              <  2:48429/148‑1 (MQ=255)
                                                                                                                                        cccTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTgg          >  1:137085/1‑148 (MQ=255)
                                                                                                                                                gCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGc  >  4:232695/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGC  >  NC_000913/1744300‑1744591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: